Cited by 100Force Microscopy Techniques and ApplicationsCellular Mechanics and InteractionsAdvanced Fluorescence Microscopy Techniques

The applications of atomic force microscopy to vision science

Open access
Last, Julie A., Russell, Paul, Nealey, Paul F., Murphy, Christopher J.·2010·Investigative Opthalmology & Visual Science

Abstract

The atomic force microscope (AFM) is widely used in materials science and has found many applications in biological sciences but has been limited in use in vision science. The AFM can be used to image the topography of soft biological materials in their native environments. It can also be used to probe the mechanical properties of cells and extracellular matrices, including their intrinsic elastic modulus and receptor-ligand interactions. In this review, the operation of the AFM is described along with a review of how it has been thus far used in vision science. It is hoped that this review will serve to stimulate vision scientists to consider incorporating AFM as part of their research toolkit.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1167/iovs.10-5470

Cites

169 of 169 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • AFM appeal in the field of nanocarriers characterization is related to the possibility to analyze highly complex samples avoiding actions such as labeling, fixing or coating, in addition to the very high resolution, approximately of 0.1 nm.

    “AFM appeal in the field of nanocarriers characterization is related to the possibility to analyze highly complex samples avoiding actions such as labeling, fixing or coating, in addition to the very high resolution, approximately of 0.1 nm [153].”

    From Polymeric micelles in drug delivery: An insight of the techniques for their characterization and assessment in biorelevant conditions (2021) · cites this paper as [153]

    The paper’s introductory discussion says AFM can image biological materials with minimal preparation—without labeling, fixing, or coating—and gives its height resolution as approximately 1 Å (0.1 nm). It does not establish these advantages specifically for nanocarrier characterization or describe the samples as highly complex. (LLM, conf=0.85)

    Experimental — accuracy not yet measured

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Part of this paper’s reference list could not be split into single entries.

Flagged first, then by citations

3 of the 172 references couldn’t be matched to a known paper.

Research works printed but not linked: 1

  1. Zhu J, Sun RG. Applications of atomic force microscopy to the structures and functions of single protein molecule. Chin J Anal Chem. 2006;34:735-740.

Cited by

Sorted by citations

Authors
Last, J. A., Russell, P., Nealey, P. F., & Murphy, C. J.
Affiliations
  • University of Wisconsin–Madison (US)
  • the Departments of Surgical and Radiological Sciences, School of Veterinary Medicine, and
  • University of California, Davis (US)
Corresponding author
  • Last, Julie A.
  • Russell, Paul
  • Nealey, Paul F.
  • Murphy, Christopher J.
Venue
Investigative Opthalmology & Visual Science
Published
2010-12-01
Volume
51
Issue
12
Pages
6083
ISSN
  • 0146-0404 (ISSN-L)
  • 1552-5783
OpenAlex
W2090709762
Cited by
100
References
172
Citations by year
2026: 3 · 2025: 6 · 2024: 7 · 2023: 7 · 2022: 8 · 2021: 6 · 2020: 10 · 2019: 5 · 2018: 5 · 2017: 3 · 2016: 4 · 2015: 7 · 2014: 9 · 2013: 6 · 2012: 11
FWCI
0.65
Type
journal article
Language
en
Open access
Locations
  • Investigative Ophthalmology & Visual Science (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Eye Institute (R01EY016134, R01EY017367, R01EY019475)
Topics
  • Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Force Microscopy Techniques and Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
Keywords
atomic force microscopy, vision science, soft biological materials, cell mechanical properties, extracellular matrix, elastic modulus, ligand-receptor interactions
MeSH terms
Animals, Eye, Eye Diseases, Eye Proteins, Humans, Ophthalmology, Surface Properties, Microscopy, Atomic Force, Biomedical Research

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review