The applications of atomic force microscopy to vision science
Open accessAbstract
The atomic force microscope (AFM) is widely used in materials science and has found many applications in biological sciences but has been limited in use in vision science. The AFM can be used to image the topography of soft biological materials in their native environments. It can also be used to probe the mechanical properties of cells and extracellular matrices, including their intrinsic elastic modulus and receptor-ligand interactions. In this review, the operation of the AFM is described along with a review of how it has been thus far used in vision science. It is hoped that this review will serve to stimulate vision scientists to consider incorporating AFM as part of their research toolkit.
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AFM appeal in the field of nanocarriers characterization is related to the possibility to analyze highly complex samples avoiding actions such as labeling, fixing or coating, in addition to the very high resolution, approximately of 0.1 nm.
“AFM appeal in the field of nanocarriers characterization is related to the possibility to analyze highly complex samples avoiding actions such as labeling, fixing or coating, in addition to the very high resolution, approximately of 0.1 nm [153].”
From Polymeric micelles in drug delivery: An insight of the techniques for their characterization and assessment in biorelevant conditions (2021) · cites this paper as [153]
The paper’s introductory discussion says AFM can image biological materials with minimal preparation—without labeling, fixing, or coating—and gives its height resolution as approximately 1 Å (0.1 nm). It does not establish these advantages specifically for nanocarrier characterization or describe the samples as highly complex. (LLM, conf=0.85)
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- Authors
- Last, J. A., Russell, P., Nealey, P. F., & Murphy, C. J.
- Affiliations
- University of Wisconsin–Madison (US)
- the Departments of Surgical and Radiological Sciences, School of Veterinary Medicine, and
- University of California, Davis (US)
- Corresponding author
- Last, Julie A.
- Russell, Paul
- Nealey, Paul F.
- Murphy, Christopher J.
- ORCID
- Nealey, Paul F.
- Venue
- Investigative Opthalmology & Visual Science
- Published
- 2010-12-01
- Volume
- 51
- Issue
- 12
- Pages
- 6083
- ISSN
- 0146-0404 (ISSN-L)
- 1552-5783
- PMC
- PMC3055745
- OpenAlex
- W2090709762
- Cited by
- 100
- References
- 172
- Citations by year
- 2026: 3 · 2025: 6 · 2024: 7 · 2023: 7 · 2022: 8 · 2021: 6 · 2020: 10 · 2019: 5 · 2018: 5 · 2017: 3 · 2016: 4 · 2015: 7 · 2014: 9 · 2013: 6 · 2012: 11
- FWCI
- 0.65
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Investigative Ophthalmology & Visual Science (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Eye Institute (R01EY016134, R01EY017367, R01EY019475)
- Topics
- Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Force Microscopy Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Keywords
- atomic force microscopy, vision science, soft biological materials, cell mechanical properties, extracellular matrix, elastic modulus, ligand-receptor interactions
- MeSH terms
- Animals, Eye, Eye Diseases, Eye Proteins, Humans, Ophthalmology, Surface Properties, Microscopy, Atomic Force, Biomedical Research
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