Local production of the alternative pathway component factor B is sufficient to promote laser-induced choroidal neovascularization
Open accessAbstract
PURPOSE: Complement factor B (CFB) is a required component of the alternative pathway (AP) of complement, and CFB polymorphisms are associated with age-related macular degeneration (AMD) risk. Complement factor B is made in the liver, but expression has also been detected in retina and retinal pigment epithelium (RPE)-choroid. We investigated whether production of CFB by the RPE can promote AP activation in mouse choroidal neovascularization (CNV). METHODS: Transgenic mice expressing CFB under the RPE65 promoter were generated and crossed onto factor B-deficient (CFB-KO) mice. Biological activity was determined in vitro using RPE monolayers and in vivo using laser-induced CNV. Contribution of systemic CFB was investigated using CFB-KO reconstituted with CFB-sufficient serum. RESULTS: Transgenic mice (CFB-tg) expressed CFB in RPE-choroid; no CFB was detected in serum. Cultured CFB-tg RPE monolayers secreted CFB apically and basally upon exposure to oxidative stress that was biologically active. Choroidal neovascularization sizes were comparable between wild-type and CFB-tg mice, but significantly increased when compared to lesions in CFB-KO mice. Injections of CFB-sufficient serum into CFB-KO mice resulted in partial reconstitution of systemic AP activity and significantly increased CNV size. CONCLUSIONS: Mouse RPE cells express and secrete CFB sufficient to promote RPE damage and CNV. This further supports that local complement production may regulate disease processes; however, the reconstitution experiments suggest that additional components may be sequestered from the bloodstream. Understanding the process of ocular complement production and regulation will further our understanding of the AMD disease process and the requirements of a complement-based therapeutic.
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Several experiments using mouse models of laser-induced CNV have demonstrated that all complement pathways, particularly the alternative and terminal pathways, play a significant role in angiogenesis.
“Several experiments using mouse models of laser-induced CNV have demonstrated that all complement pathways, particularly the alternative and terminal pathways, play a significant role in angiogenesis [150,152,153,154,155,156,157,158,159,160,161,162,163].”
From Bruch’s Membrane: A Key Consideration with Complement-Based Therapies for Age-Related Macular Degeneration (2023) · cites this paper as [152]
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Complement factor H polymorphism in age-related macular degeneration
Klein, R. J., Zeiss, C., Chew, E. Y., Tsai, J.-Y., Sackler, R. S., Haynes, C., Henning, A. K., SanGiovanni, J. P., Mane, S. M., Mayne, S. T., et al. (2005) · Science · doi:10.1126/science.1109557 · Cited by 4,492 · see citing works
Complement factor H polymorphism and age-related macular degeneration
Edwards, A. O., Ritter, R., Abel, K. J., Manning, A., Panhuysen, C., & Farrer, L. A. (2005) · Science · doi:10.1126/science.1110189 · Cited by 2,449 · see citing works
Complement factor H variant increases the risk of age-related macular degeneration
Haines, J. L., Hauser, M. A., Schmidt, S., Scott, W. K., Olson, L. M., Gallins, P., Spencer, K. L., Kwan, S. Y., Noureddine, M., Gilbert, J. R., et al. (2005) · Science · doi:10.1126/science.1110359 · Cited by 2,424 · see citing works
Hageman, G. S., Anderson, D. H., Johnson, L. V., Hancox, L. S., Taiber, A. J., Hardisty, L. I., Hageman, J. L., Stockman, H. A., Borchardt, J. D., Gehrs, K. M., et al. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0501536102 · Cited by 1,961 · see citing works
Hageman, G. (2001) · Progress in Retinal and Eye Research · doi:10.1016/s1350-9462(01)00010-6 · Cited by 1,265 · see citing works
The AMD Genetics Clinical Study Group, Gold, B., Merriam, J. E., Zernant, J., Hancox, L. S., Taiber, A. J., Gehrs, K., Cramer, K., Neel, J., Bergeron, J., et al. (2006) · Nature Genetics · doi:10.1038/ng1750 · Cited by 1,075 · see citing works
Complement C3 variant and the risk of age-related macular degeneration
Yates, J. R. W., Sepp, T., Matharu, B. K., Khan, J. C., Thurlby, D. A., Shahid, H., Clayton, D. G., Hayward, C., Morgan, J., Wright, A. F., et al. (2007) · New England Journal of Medicine · doi:10.1056/nejmoa072618 · Cited by 829 · see citing works
Rapid Quantification of Adult and Developing Mouse Spatial Vision Using a Virtual Optomotor System
Prusky, G. T., Alam, N. M., Beekman, S., & Douglas, R. M. (2004) · Investigative Ophthalmology & Visual Science · doi:10.1167/iovs.04-0541 · Cited by 777 · see citing works
Anderson, D. H., Radeke, M. J., Gallo, N. B., Chapin, E. A., Johnson, P. T., Curletti, C. R., Hancox, L. S., Hu, J., Ebright, J. N., Malek, G., et al. (2010) · Progress in Retinal and Eye Research · doi:10.1016/j.preteyeres.2009.11.003 · Cited by 727 · see citing works
Drusen complement components C3a and C5a promote choroidal neovascularization
Nozaki, M., Raisler, B. J., Sakurai, E., Sarma, J. V., Barnum, S. R., Lambris, J. D., Chen, Y., Zhang, K., Ambati, B. K., Baffi, J. Z., et al. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0408835103 · Cited by 650 · see citing works
Li, M., Atmaca-Sonmez, P., Othman, M., Branham, K. E. H., Khanna, R., Wade, M. S., Li, Y., Liang, L., Zareparsi, S., Swaroop, A., et al. (2006) · Nature Genetics · doi:10.1038/ng1871 · Cited by 354 · see citing works
HARBOE, M., ULVUND, G., VIEN, L., FUNG, M., & MOLLNES, T. E. (2004) · Clinical and Experimental Immunology · doi:10.1111/j.1365-2249.2004.02627.x · Cited by 319 · see citing works
Seddon, J. M., Reynolds, R., Maller, J., Fagerness, J. A., Daly, M. J., & Rosner, B. (2009) · Investigative Opthalmology & Visual Science · doi:10.1167/iovs.08-3064 · Cited by 285 · see citing works
The efficiency of pooling mRNA in microarray experiments
Kendziorski, C. M. (2003) · Biostatistics · doi:10.1093/biostatistics/4.3.465 · Cited by 258 · see citing works
Hepatocyte growth factor inhibits growth of hepatocellular carcinoma cells.
Shiota, G., Rhoads, D. B., Wang, T. C., Nakamura, T., & Schmidt, E. V. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.1.373 · Cited by 251 · see citing works
Gibbs, D., Kitamoto, J., & Williams, D. S. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1130432100 · Cited by 250 · see citing works
Abrogation of the alternative complement pathway by targeted deletion of murine factor B
Matsumoto, M., Fukuda, W., Circolo, A., Goellner, J. J., Strauss‐Schoenberger, J., Wang, X., Fujita, S., Hidvégi, T., Chaplin, D., & Colten, H. R. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.16.8720 · Cited by 216 · see citing works
Complement factor H polymorphism in age-related macular degeneration
Klein, R. J., Zeiss, C. J., Chew, E. Y., Tsai, J. –., Sackler, R. S., Haynes, C. M., Henning, A. K., SanGiovanni, J. P., Manne, S., Mayne, S. T., et al. (2005) · American Journal of Ophthalmology · doi:10.1016/j.ajo.2005.06.004 · Cited by 213 · see citing works
Spencer, K. L., Hauser, M. A., Olson, L. M., Schmidt, S., Scott, W. K., Gallins, P. J., Agarwal, A., Postel, E. A., Pericak‐Vance, M. A., & Haines, J. L. (2007) · Human Molecular Genetics · doi:10.1093/hmg/ddm146 · Cited by 189 · see citing works
Oxidative Stress Renders Retinal Pigment Epithelial Cells Susceptible to Complement-mediated Injury
Thurman, J. M., Renner, B., Kunchithapautham, K., Ferreira, V. P., Pangburn, M. K., Ablonczy, Z., Tomlinson, S., Holers, V. M., & Rohrer, B. (2009) · Journal of Biological Chemistry · doi:10.1074/jbc.m808166200 · Cited by 186 · see citing works
Self-citationChen, M., Forrester, J. V., & Xu, H. (2007) · Experimental Eye Research · doi:10.1016/j.exer.2006.11.015 · Cited by 175 · see citing works
Markiewski, M. M., Mastellos, D. C., Tudoran, R., DeAngelis, R. A., Strey, C. W., Franchini, S., Wetsel, R. A., Erdei, A., & Lambris, J. D. (2004) · The Journal of Immunology · doi:10.4049/jimmunol.173.2.747 · Cited by 170 · see citing works
Rohrer, B., Long, Q., Coughlin, B., Wilson, R. B., Huang, Y., Qiao, F., Tang, P. H., Kunchithapautham, K., Gilkeson, G. S., & Tomlinson, S. (2009) · Investigative Opthalmology & Visual Science · doi:10.1167/iovs.08-2222 · Cited by 154 · see citing works
Self-citationMultiple, parallel cellular suicide mechanisms participate in photoreceptor cell death
Lohr, H. R., Kunchithapautham, K., Sharma, A. K., & Rohrer, B. (2006) · Experimental Eye Research · doi:10.1016/j.exer.2006.01.014 · Cited by 146 · see citing works
Self-citationBora, N. S., Kaliappan, S., Jha, P., Xu, Q., Sohn, J.-H., Dhaulakhandi, D. B., Kaplan, H. J., & Bora, P. S. (2006) · The Journal of Immunology · doi:10.4049/jimmunol.177.3.1872 · Cited by 124 · see citing works
Elevated membrane attack complex in human choroid with high risk complement factor H genotypes
Mullins, R. F., Dewald, A. D., Streb, L. M., Wang, K., Kuehn, M. H., & Stone, E. M. (2011) · Experimental Eye Research · doi:10.1016/j.exer.2011.06.015 · Cited by 123 · see citing works
Cashman, S. M., Ramo, K., & Kumar-Singh, R. (2011) · PLoS ONE · doi:10.1371/journal.pone.0019078 · Cited by 118 · see citing works
Chen, M., Muckersie, E., Robertson, M., Forrester, J. V., & Xu, H. (2008) · Experimental Eye Research · doi:10.1016/j.exer.2008.09.005 · Cited by 99 · see citing works
Giani, A., Thanos, A., Roh, M., Connolly, E. J., Trichonas, G., Kim, I. K., Gragoudas, E. S., Vavvas, D. G., & Miller, J. W. (2011) · Investigative Ophthalmology & Visual Science · doi:10.1167/iovs.10-6266 · Cited by 97 · see citing works
Bora, P. S., Hu, Z., Tezel, T. H., Sohn, J.-H., Kang, S. G., Cruz, J. M. C., Bora, N. S., Garen, A., & Kaplan, H. J. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0438014100 · Cited by 91 · see citing works
Eliminating Complement Factor D Reduces Photoreceptor Susceptibility to Light-Induced Damage
Rohrer, B., Guo, Y., Kunchithapautham, K., & Gilkeson, G. S. (2007) · Investigative Ophthalmology & Visual Science · doi:10.1167/iovs.07-0282 · Cited by 90 · see citing works
Self-citationHuman Blood Plasma Proteins: Structure and Function
Schaller, J., Gerber, S., Kämpfer, U., Lejon, S., & Trachsel, C. (2008) · Bern Open Repository and Information System (University of Bern) · Cited by 89 · see citing works
Are low inflammatory reactions involved in exudative age-related macular degeneration?
Lommatzsch, A. P., Hermans, P., Müller, K. D., Bornfeld, N., Bird, A. C., & Pauleikhoff, D. (2008) · Graefe s Archive for Clinical and Experimental Ophthalmology · doi:10.1007/s00417-007-0749-4 · Cited by 89 · see citing works
Downregulation of differentiation specific gene expression by oxidative stress in ARPE-19 cells.
Alizadeh, M., Wada, M., Gelfman, C. M., Handa, J. T., & Hjelmeland, L. M. (2001) · PubMed · Cited by 85 · see citing works
Murine Gammaherpesvirus 68 Encodes a Functional Regulator of Complement Activation
Kapadia, S. B., Molina, H. D., van Berkel, V. H., Speck, S. H., & Virgin, H. W. (1999) · Journal of Virology · doi:10.1128/jvi.73.9.7658-7670.1999 · Cited by 84 · see citing works
Complement expression in retinal pigment epithelial cells is modulated by activated macrophages
Luo, C., Zhao, J., Madden, A. F., Chen, M., & Xu, H. (2013) · Experimental Eye Research · doi:10.1016/j.exer.2013.04.016 · Cited by 83 · see citing works
Age as an independent risk factor for severity of experimental choroidal neovascularization.
Espinosa‐Heidmann, D. G., Suñer, I. J., Hernandez, E. P., Frazier, W. D., Csaky, K. G., & Cousins, S. W. (2002) · PubMed · Cited by 81 · see citing works
Regulated secretion of complement factor H by RPE and its role in RPE migration
Kim, Y. H., He, S., Kase, S., Kitamura, M., Ryan, S. J., & Hinton, D. R. (2009) · Graefe s Archive for Clinical and Experimental Ophthalmology · doi:10.1007/s00417-009-1049-y · Cited by 76 · see citing works
Campa, C., Kasman, I. M., Ye, W., Lee, W. P., Fuh, G., & Ferrara, N. (2008) · Investigative Ophthalmology & Visual Science · doi:10.1167/iovs.07-1194 · Cited by 71 · see citing works
Joseph, K., Kulik, L. N., Coughlin, B. A., Kunchithapautham, K., Bandyopadhyay, M., Thiel, S., Thielens, N. M., Holers, V. M., & Rohrer, B. (2013) · Journal of Biological Chemistry · doi:10.1074/jbc.m112.421891 · Cited by 66 · see citing works
Self-citationA Novel Role of Complement in Retinal Degeneration
Yu, M., Zou, W., Peachey, N. S., McIntyre, T. M., & Liu, J. (2012) · Investigative Ophthalmology & Visual Science · doi:10.1167/iovs.12-10069 · Cited by 63 · see citing works
Rohrer, B., Coughlin, B., Kunchithapautham, K., Long, Q., Tomlinson, S., Takahashi, K., & Holers, V. M. (2011) · Molecular Immunology · doi:10.1016/j.molimm.2010.12.016 · Cited by 62 · see citing works
Self-citationHageman, G. S., Gehrs, K. M., Lejnine, S., Bansal, A. T., DeAngelis, M. M., Guymer, R. H., Baird, P. N., Allikmets, R. L., Deciu, C., Oeth, P. A., et al. (2011) · Human Genomics · doi:10.1186/1479-7364-5-5-420 · Cited by 57 · see citing works
Bandyopadhyay, M., & Rohrer, B. (2012) · Investigative Ophthalmology & Visual Science · doi:10.1167/iovs.11-8638 · Cited by 55 · see citing works
Self-citationWoodell, A. S., Coughlin, B. A., Kunchithapautham, K., Casey, S. E., Williamson, T., Ferrell, W. D., Atkinson, C., Jones, B. W., & Rohrer, B. (2013) · PLoS ONE · doi:10.1371/journal.pone.0067894 · Cited by 52 · see citing works
Self-citationBoulanger, A., Liu, S., Henningsgaard, A. A., Yu, S., & Redmond, T. M. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m003441200 · Cited by 50 · see citing works
Khandhadia, S., Hakobyan, S., Heng, L. Z., Gibson, J., Adams, D. H., Alexander, G. J., Gibson, J. M., Martin, K. R., Menon, G., Nash, K., et al. (2013) · Ophthalmology · doi:10.1016/j.ophtha.2013.01.004 · Cited by 47 · see citing works
Harboe, M., Garred, P., Borgen, M. S., Stahl, G. L., Roos, A., & Mollnes, T. E. (2006) · Clinical & Experimental Immunology · doi:10.1111/j.1365-2249.2006.03072.x · Cited by 30 · see citing works
Tong, H. H., Li, Y. X., Stahl, G. L., & Thurman, J. M. (2010) · Infection and Immunity · doi:10.1128/iai.01012-09 · Cited by 30 · see citing works
Schnabolk, G., Stauffer, K. M., O’Quinn, E. C., Coughlin, B. A., Kunchithapautham, K., & Rohrer, B. (2014) · Experimental Eye Research · doi:10.1016/j.exer.2014.10.005 · Cited by 19 · see citing works
Self-citationGene dosage effect of the TrkB receptor on rod physiology and biochemistry in juvenile mouse retina.
Rohrer, B. (2001) · PubMed · Cited by 19 · see citing works
Self-citationSchaller, J., Gerber, S., Kämpfer, U., Lejon, S., & Trachsel, C. (2008) · doi:10.1002/9780470724378.ch8 · Cited by 2 · see citing works
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Inflammation and its role in age-related macular degeneration
Kauppinen, A., Paterno, J. J., Błasiak, J., Salminen, A., & Kaarniranta, K. (2016) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-016-2147-8 · Cited by 660 · see citing works
Clinical promise of next-generation complement therapeutics
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Toomey, C. B., Johnson, L. V., & Rickman, C. B. (2017) · Progress in Retinal and Eye Research · doi:10.1016/j.preteyeres.2017.09.001 · Cited by 160 · see citing works
Targeting complement components C3 and C5 for the retina: Key concepts and lingering questions
Kim, B. J., Mastellos, D. C., Li, Y., Dunaief, J. L., & Lambris, J. D. (2020) · Progress in Retinal and Eye Research · doi:10.1016/j.preteyeres.2020.100936 · Cited by 91 · see citing works
Obert, E., Strauss, R. E., Brandon, C. S., Grek, C. L., Ghatnekar, G. S., Gourdie, R. G., & Rohrer, B. (2017) · Journal of Molecular Medicine · doi:10.1007/s00109-017-1506-8 · Cited by 65 · see citing works
Self-citationComplement System and Potential Therapeutics in Age-Related Macular Degeneration
Park, Y. G., Park, Y. S., & Kim, I. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22136851 · Cited by 50 · see citing works
Hammadi, S., Tzoumas, N., Ferrara, M., Meschede, I. P., Lo, K., Harris, C., Lako, M., & Steel, D. H. (2023) · Journal of Clinical Medicine · doi:10.3390/jcm12082870 · Cited by 44 · see citing works
Schnabolk, G., Parsons, N., Obert, E., Annamalai, B., Nasarre, C., Tomlinson, S., Lewin, A. S., & Rohrer, B. (2017) · Molecular Therapy — Methods & Clinical Development · doi:10.1016/j.omtm.2017.11.003 · Cited by 40 · see citing works
Self-citationJensen, E. G., Jakobsen, T. S., Thiel, S., Askou, A. L., & Corydon, T. J. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21249752 · Cited by 39 · see citing works
C3a Increases VEGF and Decreases PEDF mRNA Levels in Human Retinal Pigment Epithelial Cells
Long, Q., Cao, X., Bian, A., & Li, Y. (2016) · BioMed Research International · doi:10.1155/2016/6958752 · Cited by 34 · see citing works
Low‐molecular weight inhibitors of the alternative complement pathway
Schubart, A., Flohr, S., Junt, T., & Eder, J. (2022) · Immunological Reviews · doi:10.1111/imr.13143 · Cited by 31 · see citing works
Guo, Z., Chen, J., Zeng, Y., Wang, Z., Yao, M. C., Tomlinson, S., Chen, B., Yuan, G., & He, S. (2022) · Frontiers in Immunology · doi:10.3389/fimmu.2021.785287 · Cited by 29 · see citing works
Dong, Q., Shi, L., Li, Y., Jiang, M., Sun, H. Q., Wang, B., Cheng, H., Zhang, Y., Shao, T., Shi, Y., et al. (2018) · BMC Genomics · doi:10.1186/s12864-018-5318-1 · Cited by 27 · see citing works
Schäfer, N., Grosche, A., Schmitt, S. I., Braunger, B. M., & Pauly, D. (2017) · Frontiers in Molecular Neuroscience · doi:10.3389/fnmol.2017.00197 · Cited by 26 · see citing works
Grossman, T. R., Carrer, M., Shen, L., Johnson, R. B., Hettrick, L. A., Henry, S. P., Monia, B. P., & McCaleb, M. L. (2017) · Europe PMC (PubMed Central) · Cited by 22 · see citing works
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Murray, H., Qiu, B., Ho, S. Y., & Wang, X. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22179580 · Cited by 16 · see citing works
Annamalai, B., Parsons, N., Nicholson, C., Obert, E., Jones, B., & Rohrer, B. (2021) · Investigative Opthalmology & Visual Science · doi:10.1167/iovs.62.4.11 · Cited by 16 · see citing works
Self-citationZhang, C., Yu, Y., Yu, J.-F., Li, B.-D., Zhou, C., Yang, X., Wang, X., Wu, C., Shen, T., & Zhu, Q. (2018) · Ecotoxicology and Environmental Safety · doi:10.1016/j.ecoenv.2018.02.056 · Cited by 15 · see citing works
Annamalai, B., Parsons, N., Belhaj, M., Brandon, C. S., Potts, J. D., & Rohrer, B. (2018) · Translational Vision Science & Technology · doi:10.1167/tvst.7.2.3 · Cited by 15 · see citing works
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Cheng, X., He, D., Liao, C., Lin, S., Tang, L., Wang, Y., Hu, J., Li, W. S., Liu, Z., Wu, Y., et al. (2020) · Journal of Cellular Physiology · doi:10.1002/jcp.30103 · Cited by 14 · see citing works
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Wang, X. (2020) · International Journal of Ophthalmology · doi:10.18240/ijo.2020.03.03 · Cited by 7 · see citing works
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Gutha, R. R., Yarrappagaari, S., Thopireddy, L., Reddy, K. S., & Saddala, R. R. (2017) · Comparative Biochemistry and Physiology Part D Genomics and Proteomics · doi:10.1016/j.cbd.2017.10.005 · Cited by 6 · see citing works
Annamalai, B., Parsons, N., Nicholson, C., Joseph, K., Coughlin, B. A., Yang, X., Jones, B. W., Tomlinson, S., & Rohrer, B. (2021) · Experimental Eye Research · doi:10.1016/j.exer.2021.108583 · Cited by 6 · see citing works
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Sunamoto, M., Morohoshi, K., Sato, B., Mihashi, R., Inui, M., Yamada, M., Miyado, K., & Kawano, N. (2025) · International Journal of Molecular Sciences · doi:10.3390/ijms26031393 · Cited by 2 · see citing works
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- Authors
- Schnabolk, G., Coughlin, B., Joseph, K., Kunchithapautham, K., Bandyopadhyay, M., O'Quinn, E. C., Nowling, T., & Rohrer, B.
- Affiliations
- Ralph H. Johnson VA Medical Center (US)
- Medical University of South Carolina (US)
- Department of Ophthalmology, Medical University of South Carolina, Charleston, South Carolina, United States
- Corresponding author
- Rohrer, Bärbel
- Venue
- Investigative Opthalmology & Visual Science
- Published
- 2015-03-31
- Volume
- 56
- Issue
- 3
- Pages
- 1850
- ISSN
- 0146-0404 (ISSN-L)
- 1552-5783
- PMC
- PMC4364641
- OpenAlex
- W2063236831
- Cited by
- 37
- References
- 53
- Citations by year
- 2025: 3 · 2023: 2 · 2022: 4 · 2021: 4 · 2020: 7 · 2019: 1 · 2018: 5 · 2017: 7 · 2016: 3 · 2015: 1
- FWCI
- 5.13
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- public-domain
- Locations
- Investigative Ophthalmology & Visual Science (publishedVersion, public-domain)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- U.S. Department of Veterans Affairs (I01 RX000444)
- Research to Prevent Blindness
- Medical University of South Carolina
- Arnold and Mabel Beckman Initiative for Macular Research
- University of South Carolina
- National Institutes of Health (1c06rr015455-01a1)
- National Eye Institute (R01EY019320, R01EY024581)
- National Cancer Institute (CO6RR015455)
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (R03AR053376)
- National Center for Research Resources (C06 RR-015455)
- Topics
- Health Sciences › Medicine › Ophthalmology › Retinal Diseases and Treatments
- Life Sciences › Immunology and Microbiology › Immunology › Complement system in diseases
- Health Sciences › Medicine › Ophthalmology › Ocular Diseases and Behçet’s Syndrome
- Keywords
- factor B, alternative complement pathway, choroidal neovascularization, retinal pigment epithelium, age-related macular degeneration, oxidative stress, laser-induced CNV, transgenic mice
- MeSH terms
- Animals, Cells, Cultured, Choroid, Complement Pathway, Alternative, Disease Models, Animal, Electroretinography, Enzyme-Linked Immunosorbent Assay, Gene Expression Regulation, Lasers, Mice, Inbred C57BL, Mice, Transgenic, Complement Factor B, RNA, Messenger, Blotting, Western, Choroidal Neovascularization, Tomography, Optical Coherence, Mice, Retinal Pigment Epithelium, Real-Time Polymerase Chain Reaction
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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