Cited by 124Neutrophil, Myeloperoxidase and Oxidative MechanismsRedox biology and oxidative stressImmunodeficiency and Autoimmune Disorders

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Neutrophil-mediated oxidative burst and host defense are controlled by a Vav-PLCγ2 signaling axis in mice

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Graham, Daniel B., Robertson, Charles M., Bautista, Jhoanne, Mascarenhas, Francesca, Diacovo, M. Julia, Montgrain, Vivianne, Lam, Siu Kit, Cremasco, Viviana, Dunne, W. Michael, Faccio, Roberta, Coopersmith, Craig M., Swat, Wojciech·2007·Journal of Clinical Investigation

DOI 10.1172/jci32729PMC PMC2000813

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Abstract

Oxidative burst, a critical antimicrobial mechanism of neutrophils, involves the rapid generation and release of reactive oxygen intermediates (ROIs) by the NADPH oxidase complex. Genetic mutations in an NADPH oxidase subunit, gp91 (also referred to as NOX2), are associated with chronic granulomatous disease (CGD), which is characterized by recurrent and life-threatening microbial infections. To combat such infections, ROIs are produced by neutrophils after stimulation by integrin-dependent adhesion to the ECM in conjunction with stimulation from inflammatory mediators, or microbial components containing pathogen-associated molecular patterns. In this report, we provide genetic evidence that both the Vav family of Rho GTPase guanine nucleotide exchange factors (GEFs) and phospholipase C-gamma2 (PLC-gamma2) are critical mediators of adhesion-dependent ROI production by neutrophils in mice. We also demonstrated that Vav was critically required for neutrophil-dependent host defense against systemic infection by Staphylococcus aureus and Pseudomonas aeruginosa, 2 common pathogens associated with fatal cases of hospital-acquired pneumonia. We identified a molecular pathway in which Vav GEFs linked integrin-mediated signaling with PLC-gamma2 activation, release of intracellular Ca2+ cations, and generation of diacylglycerol to control assembly of the NADPH oxidase complex and ROI production by neutrophils. Taken together, our data indicate that integrin-dependent signals generated during neutrophil adhesion contribute to the activation of NADPH oxidase by a variety of distinct effector pathways, all of which require Vav.

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Authors: Graham, D. B., Robertson, C. M., Bautista, J., Mascarenhas, F., Diacovo, M. J., Montgrain, V., Lam, S. K., Cremasco, V., Dunne, W. M., Faccio, R., Coopersmith, C. M., & Swat, W.
Affiliations: Washington University in St. Louis (US)
Corresponding author: Swat, Wojciech
ORCID: Graham, Daniel B.
Bautista, Jhoanne
Diacovo, M. Julia
Cremasco, Viviana
Dunne, W. Michael
Faccio, Roberta
Coopersmith, Craig M.
Swat, Wojciech
Venue: Journal of Clinical Investigation
Publisher: American Society for Clinical Investigation
Published: 2007-11-01
Volume: 117
Issue: 11
Pages: 3445-3452
ISSN: 0021-9738 (ISSN-L)
DOI: 10.1172/jci32729
PMC: PMC2000813
OpenAlex: W1987998552
Cited by: 124
References: 55
Citations by year: 2026: 4 · 2025: 3 · 2024: 4 · 2023: 7 · 2021: 8 · 2020: 6 · 2019: 2 · 2018: 4 · 2017: 3 · 2016: 7 · 2015: 5 · 2014: 4 · 2013: 15 · 2012: 10
FWCI: 3.10
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Locations: Journal of Clinical Investigation (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Digital Commons@Becker (Washington University School of Medicine) (submittedVersion)
Funders: National Institutes of Health (GM072808)
National Cancer Institute (T32CA009547)
National Institute of General Medical Sciences (R01GM072808, GM 072808, GM66202, R01GM066202)
National Institute of Allergy and Infectious Diseases (AI 063024-02, AI-06107703)
Topics: Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
Life Sciences › Immunology and Microbiology › Immunology › Immunodeficiency and Autoimmune Disorders
Keywords: neutrophil respiratory burst, Vav, PLCγ2, NADPH oxidase, NOX2, integrin signaling, neutrophil adhesion, reactive oxygen species, chronic granulomatous disease, Staphylococcus aureus, Pseudomonas aeruginosa, diacylglycerol, intracellular calcium, host defense, nosocomial pneumonia
MeSH terms: Animals, Calcium, Cell Adhesion, Diglycerides, Granulomatous Disease, Chronic, Humans, Neutrophils, Pseudomonas Infections, Staphylococcal Infections, Signal Transduction, Respiratory Burst, Reactive Oxygen Species, Mice, Knockout, Pneumonia, Bacterial, Sepsis, NADPH Oxidases, Protein Subunits, Mice, Proto-Oncogene Proteins c-vav, Phospholipase C gamma

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Neutrophil-mediated oxidative burst and host defense are controlled by a Vav-PLCγ2 signaling axis in mice

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