Cited by 16Animal Virus Infections StudiesVirology and Viral DiseasesViral Infections and Immunology Research

Molecular characterization of encephalomyocarditis virus strains isolated from an African elephant and rats in a French zoo

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Romey, Aurore, Lamglait, Benjamin, Blanchard, Yannick, Touzain, Fabrice, Quenault, Helene, Relmy, Anthony, Zientara, Stephan, Blaise-Boisseau, Sandra +1 more·2021·Journal of Veterinary Diagnostic Investigation

Abstract

In November 2013, a fatal encephalomyocarditis virus (EMCV) case in a captive African elephant ( Loxodonta africana) occurred at the Réserve Africaine de Sigean, a zoo in the south of France. Here we report the molecular characterization of the EMCV strains isolated from samples collected from the dead elephant and from 3 rats ( Rattus rattus) captured in the zoo at the same time. The EMCV infection was confirmed by reverse-transcription real-time PCR (RT-rtPCR) and genome sequencing. Complete genome sequencing and sequence alignment indicated that the elephant’s EMCV strain was 98.1–99.9% identical to the rat EMCV isolates at the nucleotide sequence level. Phylogenetic analysis of the ORF, P1, VP1, and 3D sequences revealed that the elephant and rat strains clustered into lineage A of the EMCV 1 group. To our knowledge, molecular characterization of EMCV in France and Europe has not been reported previously in a captive elephant. The full genome analyses of EMCV isolated from an elephant and rats in the same outbreak emphasizes the role of rodents in EMCV introduction and circulation in zoos.

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Crossref · 10.1177/1040638720978389

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Authors
Romey, A., Lamglait, B., Blanchard, Y., Touzain, F., Quenault, H., Relmy, A., Zientara, S., Blaise-Boisseau, S., & Bakkali-Kassimi, L.
Affiliations
  • École Nationale Vétérinaire d'Alfort (FR)
  • Centre de Recherche sur l'Environnement Alpin (CH)
  • Agence Nationale de Sécurité Sanitaire de l’Alimentation, de l’Environnement et du Travail (FR)
  • Unit of Viral Genetics and Biosafety, Ploufragan Laboratory, ANSES, Ploufragan, France
  • Animal Health Laboratory, UMR1161 Virology, INRAE, ANSES, ENVA, Paris-Est University, Maisons-Alfort, France
Venue
Journal of Veterinary Diagnostic Investigation
Published
2020-12-08
Volume
33
Issue
2
Pages
313-321
ISSN
  • 1040-6387 (ISSN-L)
  • 1943-4936
OpenAlex
W3112900788
Cited by
16
References
41
Citations by year
2026: 6 · 2025: 6 · 2024: 1 · 2023: 1 · 2022: 2
FWCI
0.54
Type
journal article
Language
en
Open access
Locations
  • Journal of Veterinary Diagnostic Investigation (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Virus Infections Studies
  • Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Viral Infections and Immunology Research
Keywords
encephalomyocarditis virus, Loxodonta africana, Rattus rattus, zoo animals, captive wildlife, viral genome sequencing, phylogenetic analysis, wildlife disease outbreak
MeSH terms
Animals, Animals, Zoo, Elephants, Encephalomyocarditis virus, Female, France, Cardiovirus Infections, Rats

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