A New Approach to Drug Discovery: High-Throughput Screening of Microbial Natural Extracts against Aspergillus fumigatus Using Resazurin
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Natural products are an inexhaustible source for drug discovery. However, the validation and selection of primary screening assays are vital to guarantee a selection of extracts or molecules with relevant pharmacological action and worthy of following up. The assay must be rapid, simple, easy to implement, and produce quick results and preferably at a low cost. In this work, we developed and validated a colorimetric microtiter assay using the resazurin viability dye. The parameters of the resazurin method for high-throughput screening (HTS) using natural extracts against Aspergillus fumigatus were optimized and set up. The extracts plus RPMI-1640 modified medium containing the spores and 0.002% resazurin were added per well. The fluorescence was read after 24 to 30 h of incubation. The resazurin proved to be as suitable as Alamar Blue for determining the minimal inhibitory concentration of different antifungals against A. fumigatus and effective to analyze fungicidal and fungistatic compounds. An HTS of 12 000 microbial extracts was carried out against two A. fumigatus strains, and 2.7% of the extracts displayed antifungal activity. Our group has been the first to use this methodology for screening a collection of natural extracts to identify compounds with antifungal activity against the medically important human pathogen A. fumigatus.
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Tetrazolium salts, MTT and XTT are often used in MIC endpoint determination for both antifungal and antibacterial microdilution assays.
“Tetrazolium salts, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and 2,3-bis {2-methoxy-4-nitro-5-[(sulfenylamino) carbonyl]-2H-tetrazolium-hydroxide} (XTT), are often used in the MIC endpoint determination for both antifungal and antibacterial microdilution assays [57], [58], [59], [60].”
From Methods for in vitro evaluating antimicrobial activity: A review (2016) · cites this paper as [59]
Cites
Zhang, J.-H., Chung, T. D. Y., & Oldenburg, K. R. (1999) · SLAS Discovery · doi:10.1177/108705719900400206 · Cited by 6,847 · see citing works
<i>Aspergillus fumigatus</i> and Aspergillosis
Latgé, J.-P. (1999) · Clinical Microbiology Reviews · doi:10.1128/cmr.12.2.310 · Cited by 2,307 · see citing works
Self-citationda Silva Ferreira, M. E., Kress, M. R. V. Z., Savoldi, M., Goldman, M. H. S., Härtl, A., Heinekamp, T., Brakhage, A. A., & Goldman, G. H. (2006) · Eukaryotic Cell · doi:10.1128/ec.5.1.207-211.2006 · Cited by 403 · see citing works
Self-citationPulmonary aspergillosis: a clinical update
Zmeili, O. S., & Soubani, A. (2007) · QJM · doi:10.1093/qjmed/hcm035 · Cited by 279 · see citing works
Update onin vitrocytotoxicity assays for drug development
Niles, A. L., Moravec, R. A., & Riss, T. (2008) · Expert Opinion on Drug Discovery · doi:10.1517/17460441.3.6.655 · Cited by 157 · see citing works
Larson, E. M., Doughman, D. J., Gregerson, D. S., & Obritsch, W. F. (1997) · PubMed · Cited by 126 · see citing works
Manavathu, E. K. (2004) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/dkh066 · Cited by 76 · see citing works
Martín‐Mazuelos, E. (2003) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/dkg384 · Cited by 71 · see citing works
A rapid resazurin bioassay for assessing the toxicity of fungicides
Fai, P. B. A., & Grant, A. (2009) · Chemosphere · doi:10.1016/j.chemosphere.2008.11.078 · Cited by 70 · see citing works
Screening for Antifungal Peptides and Their Modes of Action in Aspergillus nidulans
Mania, D., Hilpert, K., Ruden, S., Fischer, R., & Takeshita, N. (2010) · Applied and Environmental Microbiology · doi:10.1128/aem.01560-10 · Cited by 54 · see citing works
Distribution of the antifungal agents sordarins across filamentous fungi
Vicente, F., Basilio, Á., Platas, G., Collado, J., Bills, G. F., del Val, A. G., Martı́n, J., Tormo, J. R., Harris, G. H., Zink, D. L., et al. (2009) · Mycological Research · doi:10.1016/j.mycres.2009.02.011 · Cited by 53 · see citing works
Self-citationYamaguchi, H., Uchida, K., Nagino, K., & Matsunaga, T. (2002) · Journal of Infection and Chemotherapy · doi:10.1007/s10156-002-0201-y · Cited by 46 · see citing works
History of medical mycology in the united states
Espinel-Ingroff, A. V. (1996) · Clinical Microbiology Reviews · doi:10.1128/cmr.9.2.235 · Cited by 39 · see citing works
In vitro Susceptibility Testing in Aspergillus Species: An Update
Lass‐Flörl, C. (2010) · Future Microbiology · doi:10.2217/fmb.10.34 · Cited by 34 · see citing works
Morace, G., Drago, M., Scaltrito, M. M., Conti, S., Fanti, F., & Polonelli, L. (2007) · Journal of Chemotherapy · doi:10.1179/joc.2007.19.5.508 · Cited by 14 · see citing works
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Sorted by citations
Methods for in vitro evaluating antimicrobial activity: A review
Balouiri, M., Sadiki, M., & Ibnsouda, S. K. (2016) · Journal of Pharmaceutical Analysis · doi:10.1016/j.jpha.2015.11.005 · Cited by 7,264 · see citing works
Antifungal Drug Development: Challenges, Unmet Clinical Needs, and New Approaches
Roemer, T., & Krysan, D. J. (2014) · Cold Spring Harbor Perspectives in Medicine · doi:10.1101/cshperspect.a019703 · Cited by 593 · see citing works
Campoy, S., & Adrio, J. L. (2017) · Biochemical Pharmacology · doi:10.1016/j.bcp.2016.11.019 · Cited by 511 · see citing works
Das, P. E., Abu‐Yousef, I. A., Majdalawieh, A. F., Narasimhan, S., & Poltronieri, P. (2020) · Molecules · doi:10.3390/molecules25030555 · Cited by 180 · see citing works
Recent advances in natural product discovery
Luo, Y., Cobb, R. E., & Zhao, H. (2014) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2014.09.002 · Cited by 153 · see citing works
Kildgaard, S., Mansson, M., Došen, I., Klitgaard, A., Frisvad, J. C., Larsen, T. O., & Nielsen, K. F. (2014) · Marine Drugs · doi:10.3390/md12063681 · Cited by 145 · see citing works
Combined LC/UV/MS and NMR Strategies for the Dereplication of Marine Natural Products
Pérez‐Victoria, I., Martı́n, J., & Reyes, F. (2016) · Planta Medica · doi:10.1055/s-0042-101763 · Cited by 139 · see citing works
Current Screening Methodologies in Drug Discovery for Selected Human Diseases
Lage, O. M., Ramos, M. C., Calisto, R., Almeida, E., Vasconcelos, V. M., & Vicente, F. (2018) · Marine Drugs · doi:10.3390/md16080279 · Cited by 133 · see citing works
Self-citationDijck, P. V., Sjollema, J., Cammue, B. P. A., Lagrou, K., Berman, J. G., d’Enfert, C., Andes, D. R., Arendrup, M. C., Brakhage, A. A., Calderone, R., et al. (2018) · Microbial Cell · doi:10.15698/mic2018.07.638 · Cited by 129 · see citing works
Self-citationMatching the power of high throughput screening to the chemical diversity of natural products
Henrich, C. J., & Beutler, J. A. (2013) · Natural Product Reports · doi:10.1039/c3np70052f · Cited by 124 · see citing works
Elbahnasawy, M. A., Shehabeldine, A. M., Khattab, A. M., Amin, B. H., & Hashem, A. H. (2021) · Journal of Drug Delivery Science and Technology · doi:10.1016/j.jddst.2021.102401 · Cited by 118 · see citing works
Shekhova, E., Kniemeyer, O., & Brakhage, A. A. (2017) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.00978-17 · Cited by 105 · see citing works
Self-citationLacret, R., Oves‐Costales, D., Gómez, C., Díaz, C., de la Cruz, M., Pérez‐Victoria, I., Vicente, F., Genilloud, O., & Reyes, F. (2014) · Marine Drugs · doi:10.3390/md13010128 · Cited by 97 · see citing works
Self-citationDas, P. E., Majdalawieh, A. F., Abu‐Yousef, I. A., Narasimhan, S., & Poltronieri, P. (2020) · Materials · doi:10.3390/ma13040876 · Cited by 84 · see citing works
Scorzoni, L., Sangalli-Leite, F., de Lacorte Singulani, J., de Paula e Silva, A. C. A., Costa-Orlandi, C. B., Fusco-Almeida, A. M., & Mendes-Giannini, M. J. S. (2016) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2016.02.005 · Cited by 82 · see citing works
Graça, A. P., Bondoso, J., Gaspar, H., Xavier, J. R., Monteiro, M. C., de la Cruz, M., Oves‐Costales, D., Vicente, F., & Lage, O. M. (2013) · PLoS ONE · doi:10.1371/journal.pone.0078992 · Cited by 82 · see citing works
Self-citationCruz, D. J. M., Bonotto, R. M., Gomes, R. G. B., da Silva, C. T., Taniguchi, J. B., No, J. H., Lombardot, B., Schwartz, O., Hansen, M. A. E., & Freitas-Junior, L. H. (2013) · PLoS neglected tropical diseases · doi:10.1371/journal.pntd.0002471 · Cited by 77 · see citing works
Xie, L., Zang, X., Cheng, W., Zhang, Z., Zhou, J., Chen, M., & Tang, Y. (2021) · Journal of the American Chemical Society · doi:10.1021/jacs.1c03988 · Cited by 70 · see citing works
Microbial cell factories based on filamentous bacteria, yeasts, and fungi
Ding, Q., & Ye, C. (2023) · Microbial Cell Factories · doi:10.1186/s12934-023-02025-1 · Cited by 66 · see citing works
Dectin-1-Targeted Antifungal Liposomes Exhibit Enhanced Efficacy
Ambati, S., Ferraro, A. R., Kang, S., Lin, J., Lin, X., Momany, M., Lewis, Z., & Meagher, R. B. (2019) · mSphere · doi:10.1128/msphere.00025-19 · Cited by 59 · see citing works
González-Menéndez, V., Crespo, G., de Pedro, N., Díaz, C., Martı́n, J., Serrano, R., Mackenzie, T. A., Justicia, C., González-Tejero, M. R., Casares, M., et al. (2018) · Scientific Reports · doi:10.1038/s41598-018-28192-5 · Cited by 54 · see citing works
Self-citationOrtiz‐López, F. J., Monteiro, M. C., González-Menéndez, V., Tormo, J. R., Genilloud, O., Bills, G. F., Vicente, F., Zhang, C., Roemer, T., Singh, S. B., et al. (2015) · Journal of Natural Products · doi:10.1021/np500854j · Cited by 50 · see citing works
Self-citationFatima, S., Farzeen, I., Ashraf, A., Aslam, B., Ijaz, M. U., Hayat, S., Sarfraz, M. H., Zafar, S., Zafar, N., Unuofin, J. O., et al. (2023) · Molecules · doi:10.3390/molecules28083469 · Cited by 48 · see citing works
Paytubi, S., de la Cruz, M., Tormo, J. R., Martı́n, J., González, I., González-Menéndez, V., Genilloud, O., Reyes, F., Vicente, F., Madrid, C., et al. (2017) · Frontiers in Microbiology · doi:10.3389/fmicb.2017.00326 · Cited by 46 · see citing works
Self-citationBioactivities and Extract Dereplication of Actinomycetales Isolated From Marine Sponges
dos Santos, J. D. N., Vitorino, I. R., de la Cruz, M., Díaz, C., Cautain, B., Annang, F. B., Pérez‐Moreno, G., González, I., Tormo, J. R., Martı́n, J., et al. (2019) · Frontiers in Microbiology · doi:10.3389/fmicb.2019.00727 · Cited by 47 · see citing works
Self-citationMirhakkak, M. H., Chen, X., Ni, Y., Heinekamp, T., Sae-Ong, T., Xu, L.-L., Kurzai, O., Barber, A. E., Brakhage, A. A., Boutin, S., et al. (2023) · Nature Communications · doi:10.1038/s41467-023-39982-5 · Cited by 45 · see citing works
Self-citationLi, Y., Chen, L., Yue, Q., Liu, X., An, Z., & Bills, G. F. (2015) · ACS Chemical Biology · doi:10.1021/acschembio.5b00013 · Cited by 43 · see citing works
Microplate alamarBlue Assay for Paracoccidioides Susceptibility Testing
Silva, A. C. A. D. P. E., de Oliveira, H. C., da Silva, J. D. F., Sangalli-Leite, F., Scorzoni, L., Fusco‐Almeida, A. M., & Mendes‐Giannini, M. J. S. (2013) · Journal of Clinical Microbiology · doi:10.1128/jcm.02914-12 · Cited by 42 · see citing works
Simple fluorescence-based high throughput cell viability assay for filamentous fungi
Chadha, S., & Kale, S. P. (2015) · Letters in Applied Microbiology · doi:10.1111/lam.12460 · Cited by 41 · see citing works
Valiante, V., Monteiro, M. C., Martı́n, J., Altwasser, R., Aouad, N. E., González, I., Kniemeyer, O., Mellado, E., Palomo, S., de Pedro, N., et al. (2015) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.00683-15 · Cited by 40 · see citing works
Self-citationNew and Improved Techniques for the Study of Pathogenic Fungi
Cairns, T. C., Studholme, D. J., Talbot, N. J., & Haynes, K. (2015) · Trends in Microbiology · doi:10.1016/j.tim.2015.09.008 · Cited by 41 · see citing works
Kildgaard, S., Subko, K., Phillips, E. S., Goidts, V., de la Cruz, M., Díaz, C., Gotfredsen, C. H., Andersen, B., Frisvad, J. C., Nielsen, K. F., et al. (2017) · Marine Drugs · doi:10.3390/md15080253 · Cited by 40 · see citing works
New Napyradiomycin Analogues from Streptomyces sp. Strain CA-271078
Carretero‐Molina, D., Ortiz‐López, F. J., Martı́n, J., Oves‐Costales, D., Díaz, C., de la Cruz, M., Cautain, B., Vicente, F., Genilloud, O., & Reyes, F. (2019) · Marine Drugs · doi:10.3390/md18010022 · Cited by 37 · see citing works
Self-citationKassab, R. M., Gomha, S. M., Al‐Hussain, S. A., Dena, A. S. A., Abdel‐Aziz, M. M., Zaki, M. E. A., & Muhammad, Z. A. (2021) · Arabian Journal of Chemistry · doi:10.1016/j.arabjc.2021.103396 · Cited by 37 · see citing works
Vartak, R., Patki, M., Menon, S., Jablonski, J. A., Mediouni, S., Fu, Y., Valente, S. T., Billack, B., & Patel, K. R. (2020) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2020.119863 · Cited by 36 · see citing works
Amrulloh, H., Fatiqin, A., Simanjuntak, W., Afriyani, H., & Annissa, A. (2021) · Advances in Natural Sciences Nanoscience and Nanotechnology · doi:10.1088/2043-6254/abde39 · Cited by 36 · see citing works
MDN-0170, a New Napyradiomycin from Streptomyces sp. Strain CA-271078
Lacret, R., Pérez‐Victoria, I., Oves‐Costales, D., de la Cruz, M., Domingo-Contreras, E., Martı́n, J., Díaz, C., Vicente, F., Genilloud, O., & Reyes, F. (2016) · Marine Drugs · doi:10.3390/md14100188 · Cited by 33 · see citing works
Self-citationde Sousa, N. S. O., Cortez, A. C. A., Melhem, M. D. S. C., Frickmann, H., & de Souza, J. V. B. (2020) · Brazilian Journal of Microbiology · doi:10.1007/s42770-020-00354-6 · Cited by 33 · see citing works
In vitro effectiveness of Brazilian brown propolis against Enterococcus faecalis
PIMENTA, H. C., Violante, I. M. P., Musis, C. R. D., Borges, Á. H., & Aranha, A. M. F. (2015) · Brazilian Oral Research · doi:10.1590/1807-3107bor-2015.vol29.0058 · Cited by 33 · see citing works
Moshawih, S., Goh, H. P., Kifli, N., Idris, A. C., Yassin, H., Kotra, V., Goh, K. W., Liew, K. B., & Ming, L. C. (2022) · Chemical Biology & Drug Design · doi:10.1111/cbdd.14062 · Cited by 31 · see citing works
Janevska, S., Ferling, I., Jojić, K., Rautschek, J., Hoefgen, S., Proctor, R. H., Hillmann, F., & Valiante, V. (2020) · mBio · doi:10.1128/mbio.00455-20 · Cited by 30 · see citing works
Self-citationGraminin B, a furanone from the fungus Paraconiothyrium sp.
Almeida, C., Aouad, N. E., Martı́n, J., Pérez‐Victoria, I., González-Menéndez, V., Platas, G., de la Cruz, M., Monteiro, M. C., de Pedro, N., Bills, G. F., et al. (2014) · The Journal of Antibiotics · doi:10.1038/ja.2014.11 · Cited by 30 · see citing works
Self-citationGonzález-Menéndez, V., Asensio, F., Moreno, C., de Pedro, N., Monteiro, M. C., de la Cruz, M., Vicente, F., Bills, G. F., Reyes, F., Genilloud, O., et al. (2014) · Mycology: An International Journal on Fungal Biology · doi:10.1080/21501203.2014.942406 · Cited by 27 · see citing works
Self-citationMitochondrial Fragmentation in Aspergillus fumigatus as Early Marker of Granulocyte Killing Activity
Ruf, D., Brantl, V., & Wagener, J. (2018) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2018.00128 · Cited by 27 · see citing works
Bioactivity Screening and Gene-Trait Matching across Marine Sponge-Associated Bacteria
Gavriilidou, A., Mackenzie, T. A., Sánchez, P., Tormo, J. R., Ingham, C. J., Smidt, H., & Sipkema, D. (2021) · Marine Drugs · doi:10.3390/md19020075 · Cited by 26 · see citing works
Self-citation<i>Aspergillus fumigatus</i> Can Display Persistence to the Fungicidal Drug Voriconazole
Scott, J., Valero, C., Mato-López, Á., Donaldson, I. J., Roldán, A., Chown, H., Van Rhijn, N., Lobo-Vega, R., Gago, S., Furukawa, T., et al. (2023) · Microbiology Spectrum · doi:10.1128/spectrum.04770-22 · Cited by 24 · see citing works
Self-citationÁlvarez, M., Núñez, F., Delgado, J., Andrade, M. J., Rodrı́guez, M., & Rodríguez, A. (2020) · Fungal Biology · doi:10.1016/j.funbio.2020.03.006 · Cited by 24 · see citing works
Kroll, K., Shekhova, E., Mattern, D. J., Thywissen, A., Jacobsen, I. D., Strassburger, M., Heinekamp, T., Shelest, E., Brakhage, A. A., & Kniemeyer, O. (2016) · Molecular Microbiology · doi:10.1111/mmi.13377 · Cited by 22 · see citing works
Self-citationCaspofungin Functionalized Polymethacrylates with Antifungal Properties
Alex, J., González, K., Kindel, T., Bellstedt, P., Weber, C., Heinekamp, T., Orasch, T., Guerrero‐Sánchez, C., Schubert, U. S., & Brakhage, A. A. (2020) · Biomacromolecules · doi:10.1021/acs.biomac.0c00096 · Cited by 22 · see citing works
Self-citationYue, Q., Li, Y., Chen, L., Zhang, X., Liu, X., An, Z., & Bills, G. F. (2018) · Environmental Microbiology · doi:10.1111/1462-2920.14089 · Cited by 22 · see citing works
Screening of Marine Bioactive Antimicrobial Compounds for Plant Pathogens
Li, X., Zhao, H., & Chen, X. (2021) · Marine Drugs · doi:10.3390/md19020069 · Cited by 21 · see citing works
Diketopiperazines and other bioactive compounds from bacterial symbionts of marine sponges
dos Santos, J. D. N., Vitorino, I. R., de la Cruz, M., Díaz, C., Cautain, B., Annang, F. B., Pérez‐Moreno, G., González, I., Tormo, J. R., Martı́n, J., et al. (2020) · Antonie van Leeuwenhoek · doi:10.1007/s10482-020-01398-2 · Cited by 21 · see citing works
Self-citationCalvo, L. G., Villarino, R.-A., Rama, J. L. R., Abril, A. G., & de Miguel, T. (2024) · LWT · doi:10.1016/j.lwt.2024.117259 · Cited by 20 · see citing works
Antifungal drug screening: thinking outside the box to identify novel antifungal scaffolds
Beattie, S. R., & Krysan, D. J. (2020) · Current Opinion in Microbiology · doi:10.1016/j.mib.2020.03.005 · Cited by 20 · see citing works
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Subko, K., Kildgaard, S., Vicente, F., Reyes, F., Genilloud, O., & Larsen, T. O. (2021) · Marine Drugs · doi:10.3390/md19020046 · Cited by 19 · see citing works
Self-citationBeattie, S. R., & Krysan, D. J. (2021) · mSphere · doi:10.1128/msphere.00539-21 · Cited by 16 · see citing works
Proteomic profiling of the antifungal drug response of Aspergillus fumigatus to voriconazole
Amarsaikhan, N., Albrecht-Eckardt, D., Sasse, C., Braus, G. H., Ogel, Z. B., & Kniemeyer, O. (2017) · International Journal of Medical Microbiology · doi:10.1016/j.ijmm.2017.07.011 · Cited by 16 · see citing works
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Lacret, R., Oves‐Costales, D., Pérez‐Victoria, I., de la Cruz, M., Díaz, C., Vicente, F., Genilloud, O., & Reyes, F. (2018) · Natural Product Research · doi:10.1080/14786419.2018.1434636 · Cited by 16 · see citing works
Self-citationBrantl, V., Boysen, J. M., Yap, A., Golubtsov, E., Ruf, D., Heinekamp, T., Straßburger, M., Dichtl, K., Haas, H., Hillmann, F., et al. (2021) · mBio · doi:10.1128/mbio.00976-21 · Cited by 15 · see citing works
Volcão, L. M., Halicki, P. C. B., Christ-Ribeiro, A., Ramos, D. F., Badiale-Furlong, E., Andreazza, R., Bernardi, E., & Júnior, F. M. R. D. S. (2021) · Journal of Toxicology and Environmental Health · doi:10.1080/15287394.2021.1970065 · Cited by 15 · see citing works
Micro- and Nanoscale Approaches in Antifungal Drug Discovery
Willaert, R. (2018) · Fermentation · doi:10.3390/fermentation4020043 · Cited by 15 · see citing works
Gomes, P. W. P., Pamplona, T. C. D. L., Navegantes-Lima, K. C., Quadros, L. B. G., Oliveira, A., dos Santos, S. M., Silva, C. Y. Y. E., Silva, M. J. C., de Souza, J. N. S., Quirós-Guerrero, L.-M., et al. (2021) · Arabian Journal of Chemistry · doi:10.1016/j.arabjc.2021.103183 · Cited by 13 · see citing works
Self-citationSadanandan, B., Vijayalakshmi, V., Ashrit, P., Babu, U. V., Kumar, L. M. S., Sampath, V., Shetty, K., Joglekar, A. P., & Awaknavar, R. (2023) · PLoS ONE · doi:10.1371/journal.pone.0281035 · Cited by 17 · see citing works
Kraut‐Cohen, J., Frenkel, O., Covo, S., Marcos‐Hadad, E., Carmeli, S., Belausov, E., Minz, D., & Cytryn, E. J. (2024) · Pest Management Science · doi:10.1002/ps.7989 · Cited by 12 · see citing works
Cebrián, E., Roncero, E., Delgado, J., Núñez, F., & Rodrı́guez, M. (2023) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2023.110342 · Cited by 12 · see citing works
Herman, T. S., Goersch, C. D. S., Bocca, A. L., & Fernandes, L. (2024) · Brazilian Journal of Microbiology · doi:10.1007/s42770-024-01293-2 · Cited by 11 · see citing works
Perlatti, B., Nichols, C. B., Lan, N., Wiemann, P., Harvey, C. J. B., Alspaugh, J. A., & Bills, G. F. (2020) · Frontiers in Microbiology · doi:10.3389/fmicb.2020.01766 · Cited by 11 · see citing works
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Perlatti, B., Harris, G. H., Nichols, C. B., Ekanayake, D. I., Alspaugh, J. A., Gloer, J. B., & Bills, G. F. (2020) · Journal of Natural Products · doi:10.1021/acs.jnatprod.0c00641 · Cited by 12 · see citing works
Virués-Segovia, J. R., Reyes, F., Ruíz, S., Martı́n, J., Fernández-Pastor, I., Justicia, C., de la Cruz, M., Díaz, C., Mackenzie, T. A., Genilloud, O., et al. (2022) · Molecules · doi:10.3390/molecules27196355 · Cited by 10 · see citing works
Self-citationAcoustic droplet ejection facilitates cell-based high-throughput screenings using natural products
Mackenzie, T. A., Tormo, J. R., Cautain, B., Martínez, G., Sánchez, I., Genilloud, O., Vicente, F., & Ramos, M. C. (2023) · SLAS TECHNOLOGY · doi:10.1016/j.slast.2023.10.003 · Cited by 10 · see citing works
Self-citationTye, K.-Y., Gan, S. Y., Lim, S. E., Tan, S.-E., Chen, C.-A., & Phang, S. (2016) · Cogent Biology · doi:10.1080/23312025.2016.1225877 · Cited by 10 · see citing works
Efficacy of oleylphosphocholine (Ol PC ) in vitro and in a mouse model of invasive aspergillosis
Paulussen, C., Boulet, G., Bosschaerts, T., Cos, P., Fortin, A., & Maes, L. J. R. M. (2015) · Mycoses · doi:10.1111/myc.12286 · Cited by 9 · see citing works
Musthafa, S. A., Dabdoub, W., Sadiq, M., & Munuswamy‐Ramanujam, G. (2021) · Materials Today Proceedings · doi:10.1016/j.matpr.2021.08.062 · Cited by 9 · see citing works
Fluorescence-Based Method and a Device for Rapid Detection of Microbial Contamination
Gurramkonda, C., Mupparapu, K., Abouzeid, R., Kostov, Y. V., & Rao, G. (2014) · PDA Journal of Pharmaceutical Science and Technology · doi:10.5731/pdajpst.2014.00951 · Cited by 9 · see citing works
Souza, I. R., Bezerra, K. G. O., Oliveira, C. L., Meira, H. M., Stamford, T. C. M., Converti, A., Sarubbo, L. A., & Rufino, R. D. (2024) · Applied Sciences · doi:10.3390/app14156711 · Cited by 8 · see citing works
Kokkini, M., Oves‐Costales, D., Sánchez, P., Melguizo, Á., Mackenzie, T. A., Pérez‐Bonilla, M., Martı́n, J., Giusti, A., de Witte, P. A. M., Vicente, F., et al. (2023) · Marine Drugs · doi:10.3390/md21080443 · Cited by 8 · see citing works
Self-citationShafiq, N. E., Mahdee, A. F., & Hasan, Z. Y. M. (2024) · The Scientific World JOURNAL · doi:10.1155/2024/6658164 · Cited by 14 · see citing works
Schalk, F., Fricke, J., Um, S., Conlon, B. H., Maus, H., Jäger, N., Heinzel, T., Schirmeister, T., Poulsen, M., & Beemelmanns, C. (2021) · RSC Advances · doi:10.1039/d1ra00997d · Cited by 8 · see citing works
Fayadoglu, M., Fayadoglu, E., Er, S., Koparal, A. T., & Koparal, A. S. (2022) · Journal of Environmental Health Science and Engineering · doi:10.1007/s40201-022-00839-6 · Cited by 9 · see citing works
Park, Y., Srikantha, T., Daniels, K. J., Jacob, M. R., Agarwal, A. K., Li, X., & Soll, D. R. (2017) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.01319-17 · Cited by 8 · see citing works
Antifungal potential and biosafety of native plants from the Brazilian Restinga ecosystem
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Filho, J. A. C. (2018) · Springer eBooks · doi:10.1007/978-981-10-8064-7_15 · Cited by 8 · see citing works
Jojić, K., Gherlone, F., Cseresnyés, Z., Bissell, A. U., Hoefgen, S., Hoffmann, S., Huang, Y., Janevska, S., Figge, M. T., & Valiante, V. (2024) · mBio · doi:10.1128/mbio.00195-24 · Cited by 7 · see citing works
Self-citationZhang, C., Li, M., Chen, G., & Guo, M. (2019) · Journal of Chromatography A · doi:10.1016/j.chroma.2019.04.039 · Cited by 7 · see citing works
Ho, F. K.-H., Delgado-Charro, M. B., & Bolhuis, A. (2019) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2019.105722 · Cited by 7 · see citing works
Serrano, R., González-Menéndez, V., Tormo, J. R., & Genilloud, O. (2023) · Journal of Fungi · doi:10.3390/jof9090883 · Cited by 6 · see citing works
Self-citationLee, S. R., Dayras, M., Fricke, J., Guo, H., Balluff, S., Schalk, F., Yu, J. S., Jeong, S. Y., Morgenstern, B., Slippers, B., et al. (2024) · Communications Chemistry · doi:10.1038/s42004-024-01210-6 · Cited by 6 · see citing works
Miguel-Gordo, M., Reddy, M. M., Sánchez, P., Buckley, J. J., Mackenzie, T. A., Jennings, L. K., Reyes, F., Calabro, K., & Thomas, O. (2022) · Organic & Biomolecular Chemistry · doi:10.1039/d1ob02297k · Cited by 6 · see citing works
Krithika, S., Sadiq, M., Munuswamy‐Ramanujam, G., & Maruthapillai, A. (2021) · Materials Today Proceedings · doi:10.1016/j.matpr.2021.07.136 · Cited by 6 · see citing works
Hoefgen, S., Bissell, A. U., Huang, Y., Gherlone, F., Raguž, L., Beemelmanns, C., & Valiante, V. (2022) · Microbiology Spectrum · doi:10.1128/spectrum.01331-22 · Cited by 5 · see citing works
Self-citationDogra, D., JULKA, J., & Kumar, A. (2021) · Asian Journal of Pharmaceutical and Clinical Research · doi:10.22159/ajpcr.2022.v15i1.43411 · Cited by 5 · see citing works
Nguyen, P. T., Wacker, T., Brown, A. J. P., Dantas, A. D. S., & Shekhova, E. (2022) · Journal of Fungi · doi:10.3390/jof8070736 · Cited by 6 · see citing works
Duarte, J., da Silva, G. A. R., Machado, F. P., Folly, D., Peñaloza, E. M. C., Garrett, R., Santos, M. G., Ventura, J. A., Wermelinger, G. F., Robbs, B. K., et al. (2022) · Food Control · doi:10.1016/j.foodcont.2022.109501 · Cited by 5 · see citing works
Evaluation of Antibacterial Assays for Screening of Marine Invertebrate Extracts
Fajarningsih, N. D., Munifah, I., & Zilda, D. S. (2018) · SQUALEN Bulletin of Marine and Fisheries Postharvest and Biotechnology · doi:10.15578/squalen.294 · Cited by 5 · see citing works
Mukherjee, D., Rooj, B., & Mandal, U. (2021) · Environmental and microbial biotechnology · doi:10.1007/978-981-15-6607-3_13 · Cited by 5 · see citing works
Sadanandan, B., Vijayalakshmi, V., Ashrit, P., Babu, U. V., Kumar, L. M. S., Sampath, V., Shetty, K., Joglekar, A. P., & Awaknavar, R. (2023) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2023.01.17.524370 · Cited by 5 · see citing works
Crespo, G., Pérez‐Victoria, I., Ortiz‐López, F. J., González-Menéndez, V., de la Cruz, M., Cautain, B., Sánchez, P., Vicente, F., Genilloud, O., & Reyes, F. (2020) · Antibiotics · doi:10.3390/antibiotics9060278 · Cited by 4 · see citing works
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Khangembam, V., Thakuria, D., Pant, V., Tandel, R., Vishwakarma, B., Pandey, N., Pande, A., & Pandey, P. (2023) · Diseases of Aquatic Organisms · doi:10.3354/dao03720 · Cited by 4 · see citing works
A Heuristic Algorithm for Plate SelectionThat Maximizes Compound Diversity
Zhu, H., Klug-McLeod, J. L., & Bakken, G. A. (2013) · Croatica Chemica Acta · doi:10.5562/cca2301 · Cited by 4 · see citing works
Antifungal Long-Chain Alkenyl Sulphates Isolated from Culture Broths of the Fungus Chaetopsina sp.
Crespo, G., González-Menéndez, V., de la Cruz, M., Martı́n, J., Cautain, B., Sánchez, P., Pérez‐Victoria, I., Vicente, F., Genilloud, O., & Reyes, F. (2016) · Planta Medica · doi:10.1055/s-0042-118190 · Cited by 4 · see citing works
Self-citation
- Authors
- Monteiro, M. C., de la Cruz, M., Cantizani, J., Moreno, C., Tormo, J. R., Mellado, E., De Lucas, J. R., Asensio, F., Valiante, V., Brakhage, A. A., Latgé, J.-P., Genilloud, O., & Vicente, F.
- Affiliations
- Fundación Medina (ES)
- Instituto de Salud Carlos III (ES)
- Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. - Hans-Knöll-Institut (HKI) (DE)
- Institut Pasteur (FR)
- Corresponding author
- Monteiro, Maria Cândida
- ORCID
- Venue
- SLAS Discovery
- Publisher
- Elsevier BV
- Published
- 2012-04
- Volume
- 17
- Issue
- 4
- Pages
- 542-549
- ISSN
- 2472-5552 (ISSN-L)
- OpenAlex
- W2012818739
- Cited by
- 144
- References
- 15
- Citations by year
- 2026: 4 · 2025: 6 · 2024: 17 · 2023: 17 · 2022: 11 · 2021: 19 · 2020: 16 · 2019: 6 · 2018: 11 · 2017: 7 · 2016: 8 · 2015: 9 · 2014: 7 · 2013: 6
- FWCI
- 4.88
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- SLAS DISCOVERY (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Funders
- Universidad de Granada
- Fundación MEDINA
- Junta de Andalucía
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Health Sciences › Medicine › Pharmacology › Microbial Natural Products and Biosynthesis
- Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
- Keywords
- Aspergillus fumigatus, resazurin, high-throughput screening, antifungal activity, minimum inhibitory concentration, drug discovery
- MeSH terms
- Antifungal Agents, Aspergillus fumigatus, Fungi, Humans, Indicators and Reagents, Microbial Sensitivity Tests, Oxazines, Xanthenes, Complex Mixtures, Drug Discovery, High-Throughput Screening Assays
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