Aspergillus fumigatus Can Display Persistence to the Fungicidal Drug Voriconazole
Open accessAbstract
The phenomena of antibacterial tolerance and persistence, where pathogenic microbes can survive for extended periods in the presence of cidal drug concentrations, have received significant attention in the last decade. Several mechanisms of action have been elucidated, and their relevance for treatment failure in bacterial infections demonstrated.
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In a Galleria mellonella mini-host model of A. fumigatus infection, fungal burden of larvae infected with persistent isolates was significantly higher than that of non-persistent isolates after voriconazole treatment.
“A study in the Galleria mellonella mini-host model of A. fumigatus infection found that fungal burden of the larvae infected with persistent isolates was significantly higher than non-persistent isolates after voriconazole treatment [4].”
From Antifungal persistence: Clinical relevance and mechanisms (2025) · cites this paper as [4]
The Results (Fig. 7) report higher mean fungal burden in voriconazole-treated larvae infected with persister isolates, but explicitly say the differences from nonpersister isolates were not significant. The claim's assertion of statistical significance contradicts that finding. (LLM, conf=0.94)
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Experimental — accuracy not yet measuredAn increase in ergosterol production and high expression of associated genes CYP51A and CDR1B were observed in voriconazole-associated A. fumigatus persistence.
“However, an increase in ergosterol production and high expression of associated genes CYP51A and CDR1B were observed in a study of voriconazole-associated A. fumigatus persistence [4].”
From Antifungal persistence: Clinical relevance and mechanisms (2025) · cites this paper as [4]
The Results section (page not provided) reports higher expression of cyp51A and cdr1B in voriconazole persisters. It presents high ergosterol production as a suggestion from gene-expression and protein-association analyses, not as an observed increase in production. (LLM, conf=0.88)
Experimental — accuracy not yet measuredIn a study on voriconazole-tolerant A. fumigatus persistence, two galactosaminogalactan (GAG) biosynthesis genes, SPH3 and UGE3, were significantly overexpressed in persisters.
“Moreover, in a study on voriconazole-tolerant A. fumigatus persistence, two galactosaminogalactan (GAG) biosynthesis genes, SPH3 and UGE3, were significantly overexpressed in persisters [4].”
From Antifungal persistence: Clinical relevance and mechanisms (2025) · cites this paper as [4]
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Self-citationMultiresistance to Nonazole Fungicides in Aspergillus fumigatus TR 34 /L98H Azole-Resistant Isolates
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Fungicidal Activity of Azole Antifungal Agents
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Research works printed but not linked: 1
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El Meouche, I., Jain, P., Jolly, M. K., & Capp, J.-P. (2024) · Translational Oncology · doi:10.1016/j.tranon.2024.102069 · Cited by 22 · see citing works
Amich, J., Bromley, M., Goldman, G. H., & Valero, C. (2025) · mBio · doi:10.1128/mbio.03475-24 · Cited by 14 · see citing works
Self-citationAntifungal persistence: Clinical relevance and mechanisms
Wang, R., Lv, J., Chen, L., Zhao, Y., & Du, H. (2025) · PLoS pathogens · doi:10.1371/journal.ppat.1013456 · Cited by 12 · see citing works
- Authors
- Scott, J., Valero, C., Mato-López, Á., Donaldson, I. J., Roldán, A., Chown, H., Van Rhijn, N., Lobo-Vega, R., Gago, S., Furukawa, T., Morogovsky, A., Ben Ami, R., Bowyer, P., Osherov, N., Fontaine, T., Goldman, G. H., Mellado, E., Bromley, M., & Amich, J.
- Affiliations
- University of Manchester (GB)
- Universidade de São Paulo (BR)
- Instituto de Salud Carlos III (ES)
- Manchester Fungal Infection Group, Division of Evolution, Infection, and Genomics, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, United Kingdom
- Tel Aviv University (IL)
- Department of Clinical Microbiology and Immunology, Sackler School of Medicine Ramat-Aviv, Tel-Aviv, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel
- Institut Pasteur (FR)
- Corresponding author
- Amich, Jorge
- ORCID
- Venue
- Microbiology Spectrum
- Publisher
- American Society for Microbiology
- Published
- 2023-04-13
- Volume
- 11
- Issue
- 2
- Pages
- e0477022-e0477022
- ISSN
- 2165-0497 (ISSN-L)
- PMC
- PMC10100717
- OpenAlex
- W4324017810
- Cited by
- 24
- References
- 150
- Citations by year
- 2026: 8 · 2025: 9 · 2024: 2 · 2023: 5
- FWCI
- 4.97
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Microbiology Spectrum (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- HAL (submittedVersion, cc-by)
- Research Explorer (The University of Manchester) (submittedVersion, other-oa)
- Funders
- British Society for Antimicrobial Chemotherapy (bsac-2016-0049)
- Comunidad de Madrid (020-T1/BMD-200)
- Fundação de Amparo à Pesquisa do Estado de São Paulo (2108/00715-3, 2020/01131-5)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- Aspergillus fumigatus, voriconazole, treatment persistence, antifungal resistance, fungal persistence, antifungal treatment failure, invasive aspergillosis
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Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
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