Cited by 24Antifungal resistance and susceptibilityFungal and yeast genetics researchBacterial Genetics and Biotechnology

Aspergillus fumigatus Can Display Persistence to the Fungicidal Drug Voriconazole

Open access
Scott, Jennifer, Valero, Clara, Mato-López, Álvaro, Donaldson, Ian J., Roldán, Alejandra, Chown, Harry, Van Rhijn, Norman, Lobo-Vega, Rebeca +11 more·2023·Microbiology Spectrum

Abstract

The phenomena of antibacterial tolerance and persistence, where pathogenic microbes can survive for extended periods in the presence of cidal drug concentrations, have received significant attention in the last decade. Several mechanisms of action have been elucidated, and their relevance for treatment failure in bacterial infections demonstrated.

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Registry

Crossref · 10.1128/spectrum.04770-22

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150 of 150 references verified0 citation errors · 0 not checkable

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  • In a Galleria mellonella mini-host model of A. fumigatus infection, fungal burden of larvae infected with persistent isolates was significantly higher than that of non-persistent isolates after voriconazole treatment.

    “A study in the Galleria mellonella mini-host model of A. fumigatus infection found that fungal burden of the larvae infected with persistent isolates was significantly higher than non-persistent isolates after voriconazole treatment [4].”

    From Antifungal persistence: Clinical relevance and mechanisms (2025) · cites this paper as [4]

    The Results (Fig. 7) report higher mean fungal burden in voriconazole-treated larvae infected with persister isolates, but explicitly say the differences from nonpersister isolates were not significant. The claim's assertion of statistical significance contradicts that finding. (LLM, conf=0.94)

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  • An increase in ergosterol production and high expression of associated genes CYP51A and CDR1B were observed in voriconazole-associated A. fumigatus persistence.

    “However, an increase in ergosterol production and high expression of associated genes CYP51A and CDR1B were observed in a study of voriconazole-associated A. fumigatus persistence [4].”

    From Antifungal persistence: Clinical relevance and mechanisms (2025) · cites this paper as [4]

    The Results section (page not provided) reports higher expression of cyp51A and cdr1B in voriconazole persisters. It presents high ergosterol production as a suggestion from gene-expression and protein-association analyses, not as an observed increase in production. (LLM, conf=0.88)

    Experimental — accuracy not yet measured
  • In a study on voriconazole-tolerant A. fumigatus persistence, two galactosaminogalactan (GAG) biosynthesis genes, SPH3 and UGE3, were significantly overexpressed in persisters.

    “Moreover, in a study on voriconazole-tolerant A. fumigatus persistence, two galactosaminogalactan (GAG) biosynthesis genes, SPH3 and UGE3, were significantly overexpressed in persisters [4].”

    From Antifungal persistence: Clinical relevance and mechanisms (2025) · cites this paper as [4]

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Research works printed but not linked: 1

  1. [148]Ortiz EM. 2019. vcf2phylip v2.0: convert a VCF matrix into several matrix formats for phylogenetic analysis (v2.0). Zenodo. CERN. doi: 10.25495/7GXK-RD71. · DOI

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Authors
Scott, J., Valero, C., Mato-López, Á., Donaldson, I. J., Roldán, A., Chown, H., Van Rhijn, N., Lobo-Vega, R., Gago, S., Furukawa, T., Morogovsky, A., Ben Ami, R., Bowyer, P., Osherov, N., Fontaine, T., Goldman, G. H., Mellado, E., Bromley, M., & Amich, J.
Affiliations
  • University of Manchester (GB)
  • Universidade de São Paulo (BR)
  • Instituto de Salud Carlos III (ES)
  • Manchester Fungal Infection Group, Division of Evolution, Infection, and Genomics, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, United Kingdom
  • Tel Aviv University (IL)
  • Department of Clinical Microbiology and Immunology, Sackler School of Medicine Ramat-Aviv, Tel-Aviv, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel
  • Institut Pasteur (FR)
Corresponding author
Amich, Jorge
Venue
Microbiology Spectrum
Published
2023-04-13
Volume
11
Issue
2
Pages
e0477022-e0477022
ISSN
2165-0497 (ISSN-L)
OpenAlex
W4324017810
Cited by
24
References
150
Citations by year
2026: 8 · 2025: 9 · 2024: 2 · 2023: 5
FWCI
4.97
Type
journal article
Language
en
Open access
Locations
  • Microbiology Spectrum (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • HAL (submittedVersion, cc-by)
  • Research Explorer (The University of Manchester) (submittedVersion, other-oa)
Funders
  • British Society for Antimicrobial Chemotherapy (bsac-2016-0049)
  • Comunidad de Madrid (020-T1/BMD-200)
  • Fundação de Amparo à Pesquisa do Estado de São Paulo (2108/00715-3, 2020/01131-5)
Topics
  • Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
Aspergillus fumigatus, voriconazole, treatment persistence, antifungal resistance, fungal persistence, antifungal treatment failure, invasive aspergillosis

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