CoChat
Sign Up
Cited by 296

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Elevated expression of IL-3Rα in acute myelogenous leukemia is associated with enhanced blast proliferation, increased cellularity, and poor prognosis

Testa, Ugo, Riccioni, Roberta, Militi, Stefania, Coccia, Eliana, Stellacci, Emilia, Samoggia, Paola, Latagliata, Roberto, Mariani, Gualtiero, Rossini, Annalisa, Battistini, Angela, Lo-Coco, Francesco, Peschle, Cesare·2002·Blood
DOI 10.1182/blood-2002-03-0852
Open this paper in CoChat — free account

Abstract

We have investigated the expression of interleukin-3 receptor α (IL-3Rα) chain in primary blasts from 79 patients with acute myeloid leukemia (AML), 25 patients with B-acute lymphoid leukemia (B-ALL), and 7 patients with T-acute lymphoid leukemia (T-ALL) to evaluate a linkage between the expression of this receptor chain, blast proliferative status, and disease prognosis. Although IL-3Rα chain was scarcely expressed in most patients with T-ALL, it was overexpressed in 40% and 45% of patients with B-ALL and AML, respectively, compared with the levels observed in normal CD34+ progenitors. The biological and clinical significance of this overexpression pattern was investigated in AML. At the biological level, elevated IL-3Rα expression was associated with peculiar properties of leukemic blasts, specifically in 3 areas. First, in all patients the blasts expressing elevated IL-3Rα levels exhibited higher cycling activity and increased resistance to apoptosis triggered by growth factor deprivation. Second, spontaneous signal transducer and activator of transcription 5 (Stat5) phosphorylation was observed in 13% of AML patients, all pertaining to the group of patients exhibiting high IL-3Rα expression. Third, following IL-3 treatment, Stat5 was activated at higher levels in blasts with elevated IL-3Rα expression. At the clinical level, a significant correlation was observed between the level of IL-3Rα expression and the number of leukemic blasts at diagnosis, and patients exhibiting elevated IL-3Rα levels had a lower complete remission rate and survival duration than those showing normal IL-3Rα levels. These findings suggest that in AML, deregulated expression of IL-3Rα may contribute to the proliferative advantage of the leukemic blasts and, hence, to a poor prognosis.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1182/blood-2002-03-0852

Cites

1 of 1 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Show all 1 references

Research works printed but not linked: 49

  1. Ogawa, 1993, Differentiation and proliferation of hematopoietic stem cells., Blood., 81, 2844, 10.1182/blood.V81.11.2844.2844 · DOI
  2. Lowenberg, 1992, Haemopoietic growth factors in acute myeloblastic and lymphoblastic leukaemia., Clin Haematol., 5, 599
  3. Bonnet, 1997, Human acute myeloid leukemia is organized as a hierarchy that originates from a primitive hematopoietic cell., Nat Med., 3, 730, 10.1038/nm0797-730 · DOI · Paper page
  4. Blair, 1998, Most acute myeloid leukemia progenitor cells with long-term proliferative ability in vitro and in vivo have the phenotype CD34+/CD71−/HLA-DR−., Blood., 92, 4325, 10.1182/blood.V92.11.4325.423k14_4325_4335 · DOI
  5. Lowenberg, 1993, Hematopoietic growth factors and their receptors in acute leukemia., Blood., 81, 281, 10.1182/blood.V81.2.281.281 · DOI
  6. Delwel, 1988, Growth regulation of human acute myeloid leukemia: effects of five recombinant hematopoietic factors in a serum-free culture system., Blood., 72, 1944, 10.1182/blood.V72.6.1944.1944 · DOI
  7. Miyauchi, 1987, The effects of three recombinant growth factors, IL-3, GM-CSF, and G-CSF, on the blast cells of acute myeloblastic leukemia maintained in short-term suspension culture., Blood., 70, 657, 10.1182/blood.V70.3.657.657 · DOI
  8. Pebusque, 1988, Growth response of human myeloid leukemia cells to colony stimulating factors., Exp Hematol., 16, 360
  9. Vellenga, 1987, Effects of recombinant IL-3, GM-CSF and G-CSF on the proliferation of leukemic clonogenic cells in short-term and long-term cultures., Leukemia., 1, 584
  10. Young, 1986, Autocrine secretion of GM-CSF in acute myeloblastic leukemia., Blood., 68, 1178, 10.1182/blood.V68.5.1178.1178 · DOI
  11. Young, 1987, Constitutive expression of the granulocyte-macrophage colony-stimulating factor gene in acute myeloblastic leukemia., J Clin Invest., 79, 100, 10.1172/JCI112769 · DOI
  12. Miyajima, 1993, Receptors for granulocyte-macrophage colony-stimulating factor, interleukin-3 and interleukin-5., Blood., 82, 1960, 10.1182/blood.V82.7.1960.1960 · DOI
  13. Bagley, 1997, The structural and functional basis of cytokine receptor activation: lessons from the common β subunit of the granulocyte-macrophage colony-stimulating factor, interleukin-3 and IL-5 receptors., Blood., 89, 1471, 10.1182/blood.V89.5.1471 · DOI
  14. Gearing, 1989, Expression cloning of a receptor for human granulocyte-macrophage colony stimulating factor., EMBO J., 8, 3667, 10.1002/j.1460-2075.1989.tb08541.x · DOI
  15. Hayashida, 1990, Molecular cloning of a second subunit of the receptor for human granulocyte-macrophage colony-stimulating factor (GM-CSF): reconstitution of a high-affinity GM-CSF receptor., Proc Natl Acad Sci U S A., 87, 9655, 10.1073/pnas.87.24.9655 · DOI
  16. Kitamura, 1991, Reconstitution of functional receptors for human granulocyte/macrophage colony stimulating factor (GM-CSF): evidence that the protein encoded by the AIC2B cDNA is a subunit of the murine GM-CSF receptor., Proc Natl Acad Sci U S A., 88, 5082, 10.1073/pnas.88.12.5082 · DOI
  17. Tavernier, 1991, A human high-affinity interleukin-5 receptor (IL-5R) is composed of an IL-5 specific alpha chain and a beta chain shared with the receptor for GM-CSF., Cell., 66, 1175, 10.1016/0092-8674(91)90040-6 · DOI
  18. Hara, 1992, Two distinct functional high-affinity receptors for mouse interleukin-3 (IL-3)., EMBO J., 11, 1875, 10.1002/j.1460-2075.1992.tb05239.x · DOI
  19. Park, 1989, Interleukin-3, GM-CSF, and G-CSF receptor expression on cell lines and primary leukemia cells: receptor heterogeneity and relationship to growth factor responsiveness., Blood., 74, 56, 10.1182/blood.V74.1.56.56 · DOI
  20. Budel, 1989, Interleukin-3 and granulocyte-monocyte colony-stimulating factor receptors on human acute myelocytic leukemia cells and relationship to the proliferative response., Blood., 74, 565, 10.1182/blood.V74.2.565.565 · DOI
  21. Budel, 1990, Common binding structure for granulocyte macrophage colony-stimulating factor and interleukin-3 on acute myeloid leukemia cells and monocytes., Blood., 75, 1439, 10.1182/blood.V75.7.1439.1439 · DOI
  22. Uckun, 1989, Leukemic B-cell precursors express functional receptors for human interleukin-3., Blood., 73, 533, 10.1182/blood.V73.2.533.533 · DOI
  23. Jordan, 2000, The interleukin-3 receptor alpha chain is a unique marker for human acute myelogenous leukemia stem cells., Leukemia., 14, 1777, 10.1038/sj.leu.2401903 · DOI
  24. Xia, 1998, Expression of signal transducers and activators of transcription proteins in acute myeloid leukemia blasts., Cancer Res., 58, 3173
  25. Hayakawa, 1998, Differential constitutive activation between STAT-related proteins and MAP kinase in primary acute myelogenous leukemia., Br J Haematol., 101, 521, 10.1046/j.1365-2141.1998.00720.x · DOI
  26. Steelman, 1996, Oncogenic effects of overexpression of the interleukin-3 receptor on hematopoietic cells., Leukemia., 10, 528
  27. Gabbianelli, 1990, Pure human hematopoietic progenitors: permissive action of basic fibroblast growth factor., Science., 249, 1561, 10.1126/science.2218497 · DOI
  28. Labbaye, 1994, Retinoic acid downmodulates erythroid differentiation and GATA-1 expression in purified adult progenitor culture., Blood., 83, 651, 10.1182/blood.V83.3.651.651 · DOI
  29. Guerriero, 1995, Unilineage megakaryocytic proliferation and differentiation of purified hematopoietic progenitors in serum-free liquid culture., Blood., 86, 3725, 10.1182/blood.V86.10.3725.bloodjournal86103725 · DOI
  30. Testa, 1993, Transforming growth factor-beta potentiates vitamin D3-induced terminal monocytic differentiation of human leukemic cell lines., J Immunol., 150, 2418, 10.4049/jimmunol.150.6.2418 · DOI
  31. Sun, 1996, Monoclonal antibody 7G3 recognizes the N-terminal domain of the human interleukin-3 (IL-3) receptor alpha-chain and functions as a specific IL-3 receptor antagonist., Blood., 87, 83, 10.1182/blood.V87.1.83.83 · DOI
  32. Ashmun, 1989, Monoclonal antibodies to the human CSF-1 receptor (c-fms proto-oncogene product) detect epitopes on normal mononuclear phagocytes and on human myeloid leukemic blast cells., Blood., 73, 827, 10.1182/blood.V73.3.827.827 · DOI
  33. Testa, 1996, Expression of growth factor receptors in unilineage differentiation culture of purified hematopoietic progenitors., Blood., 88, 3391, 10.1182/blood.V88.9.3391.bloodjournal8893391 · DOI
  34. Malorni, 1998, Oxidative stress leads to a rapid alteration of transferrin receptor intravesicular trafficking., Exp Cell Res., 241, 102, 10.1006/excr.1998.4020 · DOI
  35. Okuda, 1991, Internalization of the granulocyte-macrophage colony-stimulating factor receptor is not required for induction of protein tyrosine phosphorylation in human myeloid cells., Blood., 78, 1928, 10.1182/blood.V78.8.1928.1928 · DOI
  36. Homburg, 1995, Human neutrophils lose their surface Fcγ RIII and acquire Annexin V binding sites during apoptosis in vitro., Blood., 85, 532, 10.1182/blood.V85.2.532.532 · DOI
  37. Nicoletti, 1991, A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry., J Immunol Methods., 139, 271, 10.1016/0022-1759(91)90198-O · DOI
  38. Coccia, 1999, Activation and repression of the 2-5° synthetase and p21 gene promoters by IRF-1 and IRF-2., Oncogene., 18, 2129, 10.1038/sj.onc.1202536 · DOI
  39. Look, 1997, Oncogenic transcription factors in the human acute leukemias., Science., 278, 1059, 10.1126/science.278.5340.1059 · DOI
  40. Lowenberg, 1993, Autonomous proliferation of leukemic cells in vitro as a determinant of prognosis in adult acute myeloid leukemia., N Engl J Med., 328, 614, 10.1056/NEJM199303043280904 · DOI
  41. Weber-Nordt, 1996, Constitutive activation of STAT proteins in primary lymphoid and myeloid leukemia cells and in Epstein-Barr virus (EBV)-related lymphoma cell lines., Blood., 88, 809, 10.1182/blood.V88.3.809.809 · DOI
  42. Gouilleux-Gruart, 1996, STAT-related transcription factors are constitutively activated in peripheral blood cells from acute leukemia patients., Blood., 87, 1692, 10.1182/blood.V87.5.1692.1692 · DOI · Paper page
  43. Tsuzuki, 1997, Proliferative effects of several hematopoietic growth factors on acute myelogenous leukemia cells and correlation with treatment outcome., Leukemia., 11, 2125, 10.1038/sj.leu.2400870 · DOI
  44. Gomes, 2001, Highly abundant genes in the transcriptosome of human and baboon CD34 antigen-positive bone marrow cells., Blood., 98, 93, 10.1182/blood.V98.1.93 · DOI
  45. Guzman, 2001, Expression of tumor-suppressor genes interferon regulatory factor 1 and death-associated protein kinase in primitive acute myelogenous leukemia cells., Blood., 97, 2177, 10.1182/blood.V97.7.2177 · DOI · Paper page
  46. Tian, 1996, Multiple signalling pathways induced by granulocyte colony-stimulating factor involving activation of JAKs, STAT5, and/or STAT3 are required for regulation of three distinct classes of immediate early genes., Blood., 88, 4435, 10.1182/blood.V88.12.4435.bloodjournal88124435 · DOI · Paper page
  47. Steelman, 1996, Oncogenic effects of overexpression of the interleukin-3 receptor on hematopoietic cells., Leukemia., 10, 528
  48. Johnson, 1989, A lethal myeloproliferative syndrome in mice transplanted with bone marrow cells infected with a retrovirus expressing granulocyte-macrophage colony stimulating factor., EMBO J., 8, 441, 10.1002/j.1460-2075.1989.tb03396.x · DOI
  49. Passegué, 2001, Chronic myeloid leukemia with increased granulocyte progenitors in mice lacking JunB expression in the myeloid lineage., Cell., 104, 21, 10.1016/S0092-8674(01)00188-X · DOI

Cited by

Metadata

Authors
Testa, U., Riccioni, R., Militi, S., Coccia, E., Stellacci, E., Samoggia, P., Latagliata, R., Mariani, G., Rossini, A., Battistini, A., Lo-Coco, F., & Peschle, C.
Venue
Blood
Publisher
American Society of Hematology
Published
2002-10-15
Volume
100
Issue
8
Pages
2980-2988
ISSN
  • 1528-0020
  • 0006-4971
DOI
10.1182/blood-2002-03-0852
Cited by
296
References
1
Language
en

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review