Cited by 64Immunotherapy and Immune ResponsesImmune Response and InflammationT-cell and B-cell Immunology

Ligand of scavenger receptor class A indirectly induces maturation of human blood dendritic cells via production of tumor necrosis factor-α

Jin, Jun-O, Park, Hae-Young, Xu, Qi, Park, Joo-In, Zvyagintseva, Tatyana, Stonik, Valentin A., Kwak, Jong-Young·2009·Blood

Abstract

Dendritic cells (DCs) are the most potent antigen-presenting cells for naive T cells. In this study, scavenger receptor class A type I and type II (SR-A) were shown to be expressed by peripheral blood DCs (PBDCs) and monocyte-derived DCs (MDDCs). In addition, the binding of anti-SR-A antibody to these cells was lower in the presence of fucoidan, an SR-A agonist. Treatment of these DCs with fucoidan or anti-SR-A antibody markedly increased the surface expression of costimulatory molecules CD83 and major histocompatibility complex class II on the CD11c(high)CD123(low) myeloid subset of PBDCs. Furthermore, fucoidan-treated PBDCs produced tumor necrosis factor-alpha (TNF-alpha) but not IL-12p70. In addition, fucoidan-induced maturation was eliminated by pretreatment with TNF-alpha-neutralizing antibody. Finally, interferon-gamma secretion and T-cell proliferation were enhanced by coculture of T cells with fucoidan-matured PBDCs. Specific inhibitors of p38 MAPK and glycogen synthase kinase 3 suppressed TNF-alpha production and maturation of fucoidan-treated PBDCs. Moreover, MDDCs lacking SR-A failed to up-regulate CD83 expression, TNF-alpha production, and phosphorylation of p38 MAPK and glycogen synthase kinase 3-beta in the presence of fucoidan. Taken together, these results suggest that ligation of SR-A leads to induction of TNF-alpha, which subsequently induces PBDC maturation, thereby leading to enhanced T-cell stimulatory capacity.

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Crossref · 10.1182/blood-2008-10-184796

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Authors
Jin, J.-O., Park, H.-Y., Xu, Q., Park, J.-I., Zvyagintseva, T., Stonik, V. A., & Kwak, J.-Y.
Affiliations
  • Dong-A University (KR)
  • Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, Vladivostok, Russia
  • Russian Academy of Sciences (RU)
Venue
Blood
Published
2009-06-04
Volume
113
Issue
23
Pages
5839-5847
ISSN
  • 0006-4971 (ISSN-L)
  • 1528-0020
OpenAlex
W2124578651
Cited by
64
References
46
Citations by year
2025: 1 · 2024: 1 · 2023: 3 · 2022: 3 · 2021: 7 · 2020: 4 · 2019: 2 · 2018: 4 · 2017: 4 · 2016: 7 · 2015: 4 · 2014: 9 · 2013: 3 · 2012: 3
FWCI
1.72
Type
journal article
Language
en
Open access
closed
Locations
  • Blood (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
  • Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
  • Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
Keywords
scavenger receptor A, dendritic cell maturation, fucoidan, TNF-α, peripheral blood dendritic cells, monocyte-derived dendritic cells, CD83, MHC class II, p38 MAPK, glycogen synthase kinase-3β, T cell proliferation, interferon-gamma, antigen presentation
MeSH terms
Cell Differentiation, Cells, Cultured, Dendritic Cells, Humans, Ligands, Tumor Necrosis Factor-alpha, Phosphatidylinositol 3-Kinases, RNA Interference, Glycogen Synthase Kinase 3, p38 Mitogen-Activated Protein Kinases, Cell Proliferation, Scavenger Receptors, Class A

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