Ligand of scavenger receptor class A indirectly induces maturation of human blood dendritic cells via production of tumor necrosis factor-α
Abstract
Dendritic cells (DCs) are the most potent antigen-presenting cells for naive T cells. In this study, scavenger receptor class A type I and type II (SR-A) were shown to be expressed by peripheral blood DCs (PBDCs) and monocyte-derived DCs (MDDCs). In addition, the binding of anti-SR-A antibody to these cells was lower in the presence of fucoidan, an SR-A agonist. Treatment of these DCs with fucoidan or anti-SR-A antibody markedly increased the surface expression of costimulatory molecules CD83 and major histocompatibility complex class II on the CD11c(high)CD123(low) myeloid subset of PBDCs. Furthermore, fucoidan-treated PBDCs produced tumor necrosis factor-alpha (TNF-alpha) but not IL-12p70. In addition, fucoidan-induced maturation was eliminated by pretreatment with TNF-alpha-neutralizing antibody. Finally, interferon-gamma secretion and T-cell proliferation were enhanced by coculture of T cells with fucoidan-matured PBDCs. Specific inhibitors of p38 MAPK and glycogen synthase kinase 3 suppressed TNF-alpha production and maturation of fucoidan-treated PBDCs. Moreover, MDDCs lacking SR-A failed to up-regulate CD83 expression, TNF-alpha production, and phosphorylation of p38 MAPK and glycogen synthase kinase 3-beta in the presence of fucoidan. Taken together, these results suggest that ligation of SR-A leads to induction of TNF-alpha, which subsequently induces PBDC maturation, thereby leading to enhanced T-cell stimulatory capacity.
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SR-A indirectly participates in maturation of human blood dendritic cells via production of TNF followed by stimulation of T-cells.
“Ligand scavenger receptor class A (SR-A) indirectly participates in maturation of human blood dendritic cells via production of tumor necrosis factor followed by stimulation of T-cells.”
From Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects (2013) · cites this paper as [62]
Fucoidan acts as a scavenger receptor agonist and maturation is eliminated by pretreatment with TNF-neutralizing antibodies.
“Thereby, fucoidan acts as a scavenger receptor agonist and maturation is eliminated by pretreatment with TNF-neutralizing antibodies [62].”
From Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects (2013) · cites this paper as [62]
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Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects
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Self-citationFucoidans from Brown Alga Fucus evanescens: Structure and Biological Activity
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Self-citation
- Authors
- Jin, J.-O., Park, H.-Y., Xu, Q., Park, J.-I., Zvyagintseva, T., Stonik, V. A., & Kwak, J.-Y.
- Affiliations
- Dong-A University (KR)
- Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, Vladivostok, Russia
- Russian Academy of Sciences (RU)
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 2009-06-04
- Volume
- 113
- Issue
- 23
- Pages
- 5839-5847
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W2124578651
- Cited by
- 64
- References
- 46
- Citations by year
- 2025: 1 · 2024: 1 · 2023: 3 · 2022: 3 · 2021: 7 · 2020: 4 · 2019: 2 · 2018: 4 · 2017: 4 · 2016: 7 · 2015: 4 · 2014: 9 · 2013: 3 · 2012: 3
- FWCI
- 1.72
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Blood (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
- Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Keywords
- scavenger receptor A, dendritic cell maturation, fucoidan, TNF-α, peripheral blood dendritic cells, monocyte-derived dendritic cells, CD83, MHC class II, p38 MAPK, glycogen synthase kinase-3β, T cell proliferation, interferon-gamma, antigen presentation
- MeSH terms
- Cell Differentiation, Cells, Cultured, Dendritic Cells, Humans, Ligands, Tumor Necrosis Factor-alpha, Phosphatidylinositol 3-Kinases, RNA Interference, Glycogen Synthase Kinase 3, p38 Mitogen-Activated Protein Kinases, Cell Proliferation, Scavenger Receptors, Class A
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