An Unfractionated Fucoidan from Ascophyllum nodosum: Extraction, Characterization, and Apoptotic Effects in Vitro
Abstract
An unfractionated fucoidan was extracted from the brown alga Ascophyllum nodosum. Extraction of fucoidan from seaweed was carried out using an innovative low-chemical process. A combinational approach involving compositional analysis, HPAEC, IR analysis, GPC, and NMR was employed to elucidate the composition and structure of an unfractionated fucoidan from A. nodosum. This fucoidan is composed mainly of fucose (52.1%), and also galactose (6.1%), glucose (21.3%), and xylose (16.5%). Sulfate content was determined to be 19%. GPC data indicated a polydisperse fucoidan containing two main size fractions (47 and 420 kDa). NMR analyses revealed a fucoidan displaying broad, complex signals as expected for such a high molecular weight and heterogeneous polymer with resonances consistent with a fucoidan isolated previously from A. nodosum. The effects of fucoidan on the apoptosis of human colon carcinoma cells and fucoidan-mediated signaling pathways were also investigated. Fucoidan decreased cell viability and induced apoptosis of HCT116 colon carcinoma cells. Fucoidan treatment of HCT116 cells induced activation of caspases-9 and -3 and the cleavage of PARP, led to apoptotic morphological changes, and altered mitochondrial membrane permeability. These results detail the structure and biological activity of an unfractionated fucoidan from A. nodosum.
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Fucoidan from Ascophyllum nodosum induced activation of caspases-9 and -3 and cleavage of PARP, leading to apoptotic morphological changes and altered mitochondrial membrane permeability.
“Fucoidan from Ascophyllum nodosum induced the activation of caspases-9 and -3 and the cleavage of PARP led to apoptotic morphological changes and altered the mitochondrial membrane permeability [37].”
From Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects (2013) · cites this paper as [37]
Extraction and purification processes, including solvent choice and conditions such as pH, time, temperature and pressure, can structurally modify macroalgal polysaccharides.
“Furthermore, it was also observed that structural modifications to these molecules (i.e.molecular weight, degree of sulphation) can occur during the processes of extraction and purification; for instance, use of different extraction solvents and experimental conditions (such as pH, time, temperature and pressure)”
From Polysaccharides from macroalgae: Recent advances, innovative technologies and challenges in extraction and purification (2017) · cites this paper as Foley et al., 2011
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- Authors
- Foley, S. A., Szegezdi, E., Mulloy, B., Samali, A., & Tuohy, M. G.
- Affiliations
- Ollscoil na Gaillimhe – University of Galway (IE)
- Apoptosis Research Centre, School of Natural Sciences, National University of Ireland, Galway, Ireland
- National Institute for Biological Standards and Control (GB)
- Corresponding author
- Foley, Sarah A.
- Venue
- Journal of Natural Products
- Publisher
- American Chemical Society (ACS)
- Published
- 2011-08-29
- Volume
- 74
- Issue
- 9
- Pages
- 1851-1861
- ISSN
- 0163-3864 (ISSN-L)
- 1520-6025
- OpenAlex
- W2037388829
- Cited by
- 142
- References
- 73
- Citations by year
- 2026: 9 · 2025: 1 · 2024: 10 · 2023: 10 · 2022: 12 · 2021: 11 · 2020: 11 · 2019: 18 · 2018: 9 · 2017: 11 · 2016: 8 · 2015: 8 · 2014: 9 · 2013: 10 · 2012: 4
- FWCI
- 11.74
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Natural Products (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Physical Sciences › Earth and Planetary Sciences › Oceanography › Marine and coastal plant biology
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Keywords
- fucoidan, Ascophyllum nodosum, brown algae, fucose, sulfate content, polysaccharide structure, apoptosis, colon cancer, HCT116 cells, caspase-9, caspase-3, PARP cleavage, mitochondrial membrane permeability, sulfated polysaccharides
- MeSH terms
- Antineoplastic Agents, Cell Survival, Drug Screening Assays, Antitumor, Humans, Ireland, Polysaccharides, Structure-Activity Relationship, Apoptosis, Ascophyllum, HCT116 Cells, Caspase 3, Caspase 9
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