The DOI matches its Crossref record; 69 of the 70 references it cites were found in a registry or an index.
Early chromatin unfolding by RUNX1: a molecular explanation for differential requirements during specification versus maintenance of the hematopoietic gene expression program
Open accessAbstract
At the cellular level, development progresses through successive regulatory states, each characterized by their specific gene expression profile. However, the molecular mechanisms regulating first the priming and then maintenance of gene expression within one developmental pathway are essentially unknown. The hematopoietic system represents a powerful experimental model to address these questions and here we have focused on a regulatory circuit playing a central role in myelopoiesis: the transcription factor PU.1, its target gene colony-stimulating-factor 1 receptor (Csf1r), and key upstream regulators such as RUNX1. We find that during ontogeny, chromatin unfolding precedes the establishment of active histone marks and the formation of stable transcription factor complexes at the Pu.1 locus and we show that chromatin remodeling is mediated by the transient binding of RUNX1 to Pu.1 cis-elements. By contrast, chromatin reorganization of Csf1r requires prior expression of PU.1 together with RUNX1 binding. Once the full hematopoietic program is established, stable transcription factor complexes and active chromatin can be maintained without RUNX1. Our experiments therefore demonstrate how individual transcription factors function in a differentiation stage–specific manner to differentially affect the initiation versus maintenance of a developmental program.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The cited source doesn’t support the claim as written.
In the citing paper
“The best current candidates for this silencing activity include GATA-3 (104, 105), TCF-1 or LEF-1 (37), and especially Runx1 (80, 150–152), probably working in a dose-dependent combination, although the mechanism through which they finally achieve the ability to repress PU.1 has not yet been reported.”
From Mechanisms of Action of Hematopoietic Transcription Factor PU.1 in Initiation of T-Cell Development (2019) · cites this paper as [151]
Checked by a reasoning model — may be wrong.
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
A bivalent chromatin structure marks key developmental genes in embryonic stem cells
Bernstein, B. E., Mikkelsen, T. S., Xie, X., Kamal, M., Huebert, D. J., Cuff, J., Fry, B., Meissner, A., Wernig, M., Plath, K., et al. (2006) · Cell · doi:10.1016/j.cell.2006.02.041 · Cited by 5,361
Polycomb complexes repress developmental regulators in murine embryonic stem cells
Boyer, L. A., Plath, K., Zeitlinger, J., Brambrink, T., Medeiros, L. A., Lee, T. I., Levine, S. S., Wernig, M., Tajonar, A., Ray, M. K., et al. (2006) · Nature · doi:10.1038/nature04733 · Cited by 2,568
Requirement of transcription factor PU.1 in the development of multiple hematopoietic lineages
Scott, E. W., Simon, M. C., Anastasi, J., & Singh, H. (1994) · Science · doi:10.1126/science.8079170 · Cited by 1,532
Métivier, R., Penot, G., Hübner, M. R., Reid, G., Brand, H., Koš, M., & Gannon, F. (2003) · Cell · doi:10.1016/s0092-8674(03)00934-6 · Cited by 1,431
Chromatin signatures of pluripotent cell lines
Azuara, V., Perry, P., Sauer, S., Spivakov, M., Jørgensen, H. F., John, R. M., Gouti, M., Casanova, M., Warnes, G., Merkenschlager, M., et al. (2006) · Nature Cell Biology · doi:10.1038/ncb1403 · Cited by 1,357
Self-citationDai, X.-M., Ryan, G. R., Hapel, A. J., Dominguez, M. G., Russell, R. G., Kapp, S., Sylvestre, V., & Stanley, E. R. (2002) · Blood · doi:10.1182/blood.v99.1.111 · Cited by 1,184
Targeted disruption of the PU.1 gene results in multiple hematopoietic abnormalities
McKercher, S. R., Torbett, B. E., Anderson, K. L., Henkel, G. W., Vestal, D. J., Baribault, H., Klemsz, M., Feeney, A. J., Wu, G. E., Paige, C. J., et al. (1996) · The EMBO Journal · doi:10.1002/j.1460-2075.1996.tb00949.x · Cited by 1,130
Runx1 is required for the endothelial to haematopoietic cell transition but not thereafter
Chen, M. J., Yokomizo, T., Zeigler, B. M., Dzierzak, E. A., & Speck, N. A. (2009) · Nature · doi:10.1038/nature07619 · Cited by 1,030
Hyperdynamic Plasticity of Chromatin Proteins in Pluripotent Embryonic Stem Cells
Meshorer, E., Yellajoshyula, D., George, E. M., Scambler, P. J., Brown, D. T., & Misteli, T. (2006) · Developmental Cell · doi:10.1016/j.devcel.2005.10.017 · Cited by 1,008
Stepwise Reprogramming of B Cells into Macrophages
Xie, H., Ye, M., Feng, R., & Graf, T. (2004) · Cell · doi:10.1016/s0092-8674(04)00419-2 · Cited by 987
Transcriptional repression mediated by repositioning of genes to the nuclear lamina
Reddy, K. L., Zullo, J., Bertolino, E., & Singh, H. (2008) · Nature · doi:10.1038/nature06727 · Cited by 823
Kyba, M., Perlingeiro, R., & Daley, G. (2002) · Cell · doi:10.1016/s0092-8674(02)00680-3 · Cited by 822
G9a-mediated irreversible epigenetic inactivation of Oct-3/4 during early embryogenesis
Feldman, N., Gerson, A., Fang, J., Li, E., Zhang, Y., Shinkai, Y., Cedar, H., & Bergman, Y. (2006) · Nature Cell Biology · doi:10.1038/ncb1353 · Cited by 651
The haemangioblast generates haematopoietic cells through a haemogenic endothelium stage
Lancrin, C., Sroczyńska, P., Stephenson, C., Allen, T., Kouskoff, V., & Lacaud, G. (2009) · Nature · doi:10.1038/nature07679 · Cited by 651
Self-citationIdentification of in vivo DNA targets of chromatin proteins using tethered Dam methyltransferase
van Steensel, B., & Henikoff, S. (2000) · Nature Biotechnology · doi:10.1038/74487 · Cited by 637
Multilineage Transcriptional Priming and Determination of Alternate Hematopoietic Cell Fates
Laslo, P., Spooner, C. J., Warmflash, A., Lancki, D. W., Lee, H.-J., Sciammas, R., Gantner, B. N., Dinner, A. R., & Singh, H. (2006) · Cell · doi:10.1016/j.cell.2006.06.052 · Cited by 632
Zhao, X. D., Han, X., Chew, J. L., Liu, J., Chiu, K. P., Choo, A. B. H., Orlov, Y. L., Sung, W.-K. K., Shahab, A., Kuznetsov, V. A., et al. (2007) · Cell stem cell · doi:10.1016/j.stem.2007.08.004 · Cited by 626
The CBFβ Subunit Is Essential for CBFα2 (AML1) Function In Vivo
Wang, Q., Stacy, T., Miller, J. D., Lewis, A. F., Gu, T.-L., Huang, X., Bushweller, J. H., Bories, J.-C., Alt, F. W., Ryan, G., et al. (1996) · Cell · doi:10.1016/s0092-8674(00)81389-6 · Cited by 621
Ichikawa, M., Asai, T., Saito, T., Yamamoto, G., Seo, S., Yamazaki, I., Yamagata, T., Mitani, K., Chiba, S., Hirai, H., et al. (2004) · Nature Medicine · doi:10.1038/nm997 · Cited by 596
Continuous single-cell imaging of blood generation from haemogenic endothelium
Eilken, H. M., Nishikawa, S., & Schroeder, T. (2009) · Nature · doi:10.1038/nature07760 · Cited by 564
Fehling, H. J., Lacaud, G., Kubo, A., Kennedy, M., Robertson, S. M., Keller, G. M., & Kouskoff, V. (2003) · Development · doi:10.1242/dev.00589 · Cited by 506
Self-citationAcute myeloid leukemia induced by graded reduction of a lineage-specific transcription factor, PU.1.
Rosenbauer, F., Wagner, K., Kutok, J. L., Iwasaki, H., Le Beau, M. M., Okuno, Y., Akashi, K., Fiering, S., & Tenen, D. G. (2004) · Nature Genetics · doi:10.1038/ng1361 · Cited by 506
Self-citationLoss of Runx1 perturbs adult hematopoiesis and is associated with a myeloproliferative phenotype
Growney, J. D., Shigematsu, H., Li, Z., Lee, B. H., Adelsperger, J., Rowan, R., Curley, D. P., Kutok, J. L., Akashi, K., Williams, I. R., et al. (2005) · Blood · doi:10.1182/blood-2004-08-3280 · Cited by 462
Myeloid or Lymphoid Promiscuity as a Critical Step in Hematopoietic Lineage Commitment
Miyamoto, T., Iwasaki, H., Reizis, B., Ye, M., Graf, T., Weissman, I. L., & Akashi, K. (2002) · Developmental Cell · doi:10.1016/s1534-5807(02)00201-0 · Cited by 415
Differentiation plasticity of hematopoietic cells.
Graf, T. (2002) · Blood · doi:10.1182/blood.v99.9.3089 · Cited by 366
Laiosa, C. V., Stadtfeld, M., Xie, H., de Andres-Aguayo, L., & Graf, T. (2006) · Immunity · doi:10.1016/j.immuni.2006.09.011 · Cited by 351
Detection of in vivo protein–DNA interactions using DamID in mammalian cells
Vogel, M. J., Peric‐Hupkes, D., & van Steensel, B. (2007) · Nature Protocols · doi:10.1038/nprot.2007.148 · Cited by 336
CD41 expression defines the onset of primitive and definitive hematopoiesis in the murine embryo
Ferkowicz, M. J., Starr, M. D., Xie, X., Li, W., Johnson, S. A., Shelley, W. C., Morrison, P. R., & Yöder, M. C. (2003) · Development · doi:10.1242/dev.00632 · Cited by 333
Dynamic regulation of PU.1 expression in multipotent hematopoietic progenitors
Nutt, S. L., Metcalf, D., D'Amico, A., Polli, M., & Wu, L. (2005) · The Journal of Experimental Medicine · doi:10.1084/jem.20041535 · Cited by 325
Runx1 is essential for hematopoietic commitment at the hemangioblast stage of development in vitro
Lacaud, G. (2002) · Blood · doi:10.1182/blood-2001-12-0321 · Cited by 309
Self-citationDeKoter, R. P., Walsh, J. C., & Singh, H. (1998) · The EMBO Journal · doi:10.1093/emboj/17.15.4456 · Cited by 296
Iwasaki, H., Mizuno, S.-I., Arinobu, Y., Ozawa, H., Mori, Y., Shigematsu, H., Takatsu, K., Tenen, D. G., & Akashi, K. (2006) · Genes & Development · doi:10.1101/gad.1493506 · Cited by 295
Self-citationPU.1 is a major downstream target of AML1 (RUNX1) in adult mouse hematopoiesis.
Huang, G., Zhang, P., Hirai, H., Elf, S., Yan, X., Chen, Z., Koschmieder, S., Okuno, Y., Dayaram, T., Growney, J. D., et al. (2008) · Nature Genetics · doi:10.1038/ng.2007.7 · Cited by 259
Self-citationRunx1-mediated hematopoietic stem-cell emergence is controlled by a Gata/Ets/SCL-regulated enhancer
Nottingham, W. T., Jarratt, A., Burgess, M. O., Speck, C. L., Cheng, J., Prabhakar, S., Rubin, E., Li, P.-S., Sloane‐Stanley, J., Kong-a-San, J., et al. (2007) · Blood · doi:10.1182/blood-2007-07-100883 · Cited by 249
Pimanda, J. E., Ottersbach, K., Knezevic, K., Kinston, S., Chan, W. Y. I., Wilson, N. K., Landry, J.-R., Wood, A. D., Kolb-Kokocinski, A., Green, A. R., et al. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0707045104 · Cited by 228
Self-citation(1995) · PubMed · Cited by 206
Self-citationSpi-1/PU.1 Transgenic Mice Develop Multistep Erythroleukemias
Moreau-Gachelin, F., Wendling, F., Molina, T., Denis, N., Titeux, M., Grimber, G., Briand, P., Vainchenker, W., & Tavitian, A. (1996) · Molecular and Cellular Biology · doi:10.1128/mcb.16.5.2453 · Cited by 189
Enver, T., & Greaves, M. F. (1998) · Cell · doi:10.1016/s0092-8674(00)81215-5 · Cited by 188
Maier, H., Ostraat, R., Gao, H., Fields, S., Shinton, S. A., Medina, K. L., Ikawa, T., Murre, C., Singh, H., Hardy, R. R., et al. (2004) · Nature Immunology · doi:10.1038/ni1119 · Cited by 184
Potential Autoregulation of Transcription Factor PU.1 by an Upstream Regulatory Element
Okuno, Y., Huang, G., Rosenbauer, F., Evans, E. K., Radomska, H. S., Iwasaki, H., Akashi, K., Moreau-Gachelin, F., Li, Y., Zhang, P., et al. (2005) · Molecular and Cellular Biology · doi:10.1128/mcb.25.7.2832-2845.2005 · Cited by 174
Self-citationEpigenetic ontogeny of the Igk locus during B cell development
Goldmit, M., Ji, Y., Skok, J. A., Roldán, E., Jung, S., Cedar, H., & Bergman, Y. (2004) · Nature Immunology · doi:10.1038/ni1154 · Cited by 162
Porcher, C., Liao, E. C.-W., Fujiwara, Y., Zon, L. I., & Orkin, S. H. (1999) · Development · doi:10.1242/dev.126.20.4603 · Cited by 161
Expression of the human acute myeloid leukemia gene AML1 is regulated by two promoter regions.
Ghozi, M. C., Bernstein, Y., Negreanu, V., Levanon, D., & Groner, Y. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.5.1935 · Cited by 150
Attema, J. L., Papathanasiou, P., Forsberg, E. C., Xu, J., Smale, S. T., & Weissman, I. L. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0704468104 · Cited by 134
Tagoh, H., Himes, R., Clarke, D., Leenen, P. J. M., Riggs, A. D., Hume, D. A., & Bonifer, C. (2002) · Genes & Development · doi:10.1101/gad.222002 · Cited by 123
Self-citationAML1 deletion in adult mice causes splenomegaly and lymphomas
Putz, G., Rosner, A., Nuesslein, I., Schmitz, N., & Buchholz, F. (2005) · Oncogene · doi:10.1038/sj.onc.1209136 · Cited by 121
C/EBPα binds and activates the PU.1 distal enhancer to induce monocyte lineage commitment
Yeamans, C., Wang, D., Paz-Priel, I., Torbett, B. E., Tenen, D. G., & Friedman, A. D. (2007) · Blood · doi:10.1182/blood-2007-03-080291 · Cited by 118
Self-citationRegulation of the PU.1 gene by distal elements
Li, Y., Okuno, Y., Zhang, P., Radomska, H. S., Chen, H.-M., Iwasaki, H., Akashi, K., Klemsz, M. J., McKercher, S. R., Maki, R. A., et al. (2001) · Blood · doi:10.1182/blood.v98.10.2958 · Cited by 110
Self-citationHistone hypomethylation is an indicator of epigenetic plasticity in quiescent lymphocytes
Baxter, J., Sauer, S., Peters, A. H. F. M., John, R. M., Williams, R. R. E., Caparros, M.-L., Arney, K. L., Otte, A. P., Jenuwein, T., Merkenschlager, M., et al. (2004) · The EMBO Journal · doi:10.1038/sj.emboj.7600414 · Cited by 109
Self-citationKrysinska, H., Hoogenkamp, M., Ingram, R., Wilson, N., Tagoh, H., Laslo, P., Singh, H., & Bonifer, C. (2007) · Molecular and Cellular Biology · doi:10.1128/mcb.01915-06 · Cited by 102
Self-citationPerry, P., Sauer, S., Billon, N., Richardson, W. D., Spivakov, M., Warnes, G., Livesey, F. J., Merkenschlager, M., Fisher, A. G., & Azuara, V. (2004) · PubMed · Cited by 101
Self-citationTelfer, J. C., & Rothenberg, E. V. (2001) · Developmental Biology · doi:10.1006/dbio.2000.9991 · Cited by 89
Perry, P., Sauer, S., Billon, N., Richardson, W. D., Spivakov, M., Warnes, G., Livesey, F. J., Merkenschlager, M., Fisher, A. G., & Azuara, V. (2004) · Cell Cycle · doi:10.4161/cc.3.12.1346 · Cited by 85
Self-citationRunx genes are direct targets of Scl/Tal1 in the yolk sac and fetal liver
Landry, J., Kinston, S., Knezevic, K., Bruijn, M. F. T. R. D., Wilson, N. K., Nottingham, W. T., Peitz, M., Edenhofer, F., Pimanda, J. E., Ottersbach, K., et al. (2008) · Blood · doi:10.1182/blood-2007-07-098830 · Cited by 80
A highly conserved c-fmsgene intronic element controls macrophage-specific and regulated expression
Himes, S. R., Tagoh, H., Goonetilleke, N., Sasmono, R. T., Oceandy, D., Clark, R. J., Bonifer, C., & Hume, D. A. (2001) · Journal of Leukocyte Biology · doi:10.1189/jlb.70.5.812 · Cited by 79
Self-citationTagoh, H., Schebesta, A., Lefevre, P., Wilson, N. K., Hume, D. A., Busslinger, M., & Bonifer, C. (2004) · The EMBO Journal · doi:10.1038/sj.emboj.7600421 · Cited by 72
Self-citationTagoh, H., Melnik, S., Lefevre, P., Chong, S., Riggs, A. D., & Bonifer, C. (2003) · Blood · doi:10.1182/blood-2003-09-3323 · Cited by 69
Self-citationHoogenkamp, M., Krysinska, H., Ingram, R., Huang, G., Barlow, R. E. L., Clarke, D., Ebralidze, A. K., Zhang, P., Tagoh, H., Cockerill, P. N., et al. (2007) · Molecular and Cellular Biology · doi:10.1128/mcb.00905-07 · Cited by 62
Self-citationEpigenetic consequences of AML1–ETO action at the human c‐FMS locus
Follows, G., Tagoh, H., Lefevre, P., Hodge, D., Morgan, G. J., & Bonifer, C. (2003) · The EMBO Journal · doi:10.1093/emboj/cdg250 · Cited by 60
Self-citationOctamer Binding Factors and Their Coactivator Can Activate the Murine PU.1 (spi-1) Promoter
Chen, H.-M., Zhang, P., Radomska, H. S., Hetherington, C. J., Zhang, D.-E., & Tenen, D. G. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.26.15743 · Cited by 59
Self-citationGoyama, S., Yamaguchi, Y., Imai, Y., Kawazu, M., Nakagawa, M. M., Asai, T., Kumano, K., Mitani, K., Ogawa, S., Chiba, S., et al. (2004) · Blood · doi:10.1182/blood-2004-04-1535 · Cited by 57
Himes, S. R., Cronau, S. L., Mulford, C., & Hume, D. A. (2005) · Oncogene · doi:10.1038/sj.onc.1208657 · Cited by 53
Regulation of granulocyte and monocyte differentiation by CCAAT/enhancer binding protein α
Friedman, A. D., Keefer, J. R., Kummalue, T., Liu, H., Wang, Q., & Cleaves, R. (2003) · Blood Cells Molecules and Diseases · doi:10.1016/s1079-9796(03)00135-9 · Cited by 51
Lefevre, P., Melnik, S., Wilson, N. K., Riggs, A. D., & Bonifer, C. (2003) · Molecular and Cellular Biology · doi:10.1128/mcb.23.12.4386-4400.2003 · Cited by 49
Self-citationSmad1 expands the hemangioblast population within a limited developmental window
Zafonte, B. T., Liu, S. X., Lynch-Kattman, M., Torregroza, I., Benvenuto, L. J., Kennedy, M., Keller, G. M., & Evans, T. (2006) · Blood · doi:10.1182/blood-2006-02-004564 · Cited by 42
Lefevre, P., Lacroix, C., Tagoh, H., Hoogenkamp, M., Melnik, S., Ingram, R., & Bonifer, C. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m502422200 · Cited by 36
Self-citationIn Vivo Genomic Footprinting Using LM-PCR Methods
Tagoh, H., Cockerill, P. N., & Bonifer, C. (2006) · Humana Press eBooks · doi:10.1385/1-59745-005-7:285 · Cited by 26
Self-citationEpigenetic Plasticity of Hematopoietic Cells
Bonifer, C. (2004) · Cell Cycle · doi:10.4161/cc.4.2.1411 · Cited by 24
Self-citationTada, T. (2006) · Methods in molecular biology · doi:10.1007/978-1-59745-154-3_15 · Cited by 3
Cited by
Sorted by citations
Transcription factors: from enhancer binding to developmental control
Spitz, F., & Furlong, E. E. M. (2012) · Nature Reviews Genetics · doi:10.1038/nrg3207 · Cited by 2,227
Pioneer transcription factors: establishing competence for gene expression
Zaret, K. S., & Carroll, J. S. (2011) · Genes & Development · doi:10.1101/gad.176826.111 · Cited by 1,589
From haematopoietic stem cells to complex differentiation landscapes
Laurenti, E., & Göttgens, B. (2018) · Nature · doi:10.1038/nature25022 · Cited by 929
Dynamic regulation of transcriptional states by chromatin and transcription factors
Voss, T. C., & Hager, G. L. (2014) · Nature Reviews Genetics · doi:10.1038/nrg3623 · Cited by 504
Dynamic Gene Regulatory Networks Drive Hematopoietic Specification and Differentiation
Goode, D. K., Obier, N., Vijayabaskar, M. S., Lie‐A‐Ling, M., Lilly, A. J., Hannah, R., Lichtinger, M., Batta, K., Florkowska, M., Patel, R., et al. (2016) · Developmental Cell · doi:10.1016/j.devcel.2016.01.024 · Cited by 239
Self-citation
Metadata
- Authors
- Hoogenkamp, M., Lichtinger, M., Krysinska, H., Lancrin, C., Clarke, D., Williamson, A., Mazzarella, L., Ingram, R., Jorgensen, H., Fisher, A., Tenen, D. G., Kouskoff, V., Lacaud, G., & Bonifer, C.
- Affiliations
- University of Leeds (GB)
- Cancer Research UK (GB)
- Imperial College London (GB)
- Medical Research Council (MRC) Clinical Sciences Centre, Imperial College London School of Medicine, London, United Kingdom;
- Harvard University (US)
- Cancer Research UK Stem Cell Haematopoiesis Group, Paterson Institute for Cancer Research, University of Manchester, Manchester, United Kingdom;
- ORCID
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 2009-07-09
- Volume
- 114
- Issue
- 2
- Pages
- 299-309
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- PMC
- PMC2714206
- OpenAlex
- W2041514505
- Cited by
- 121
- References
- 70
- Citations by year
- 2026: 4 · 2025: 2 · 2024: 2 · 2023: 2 · 2022: 4 · 2021: 4 · 2020: 3 · 2019: 7 · 2018: 4 · 2017: 9 · 2016: 9 · 2015: 6 · 2014: 13 · 2013: 11 · 2012: 10
- FWCI
- 5.51
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Blood (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Research Explorer (The University of Manchester) (publishedVersion)
- National University of Singapore (submittedVersion)
- University of Birmingham Research Portal (University of Birmingham) (submittedVersion)
- Funders
- Cancer Research UK
- National Institutes of Health
- Directorate for Biological Sciences
- Medical Research Council
- Biotechnology and Biological Sciences Research Council (BB/F000499/1, BB/E025129/1)
- National Cancer Institute (CA 41456, R01CA041456)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Chromatin Remodeling and Cancer
- Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
- Keywords
- RUNX1, PU.1, CSF1R, chromatin unfolding, chromatin remodeling, hematopoietic differentiation, myelopoiesis, transcription factor binding, cis-regulatory elements, histone modifications, transcription factor complex, gene expression regulation
- MeSH terms
- Animals, Blood Cells, Cells, Cultured, Chromatin, Gene Expression Regulation, Promoter Regions, Genetic, Protein Binding, RNA, Messenger, Time Factors, DNA Methylation, Core Binding Factor Alpha 2 Subunit, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
