Increased uncoupling protein 3 content does not affect mitochondrial function in human skeletal muscle in vivo
Open accessAbstract
Phosphocreatine (PCr) resynthesis rate following intense anoxic contraction can be used as a sensitive index of in vivo mitochondrial function. We examined the effect of a diet-induced increase in uncoupling protein 3 (UCP3) expression on postexercise PCr resynthesis in skeletal muscle. Nine healthy male volunteers undertook 20 one-legged maximal voluntary contractions with limb blood flow occluded to deplete muscle PCr stores. Exercise was performed following 7 days consumption of low-fat (LF) or high-fat (HF) diets. Immediately following exercise, blood flow was reinstated, and muscle was sampled after 20, 60, and 120 seconds of recovery. Mitochondrial coupling was assessed by determining the rate of PCr resynthesis during recovery. The HF diet increased UCP3 protein content by approximately 44% compared with the LF diet. However, this HF diet-induced increase in UCP3 expression was not associated with any changes in the rate of muscle PCr resynthesis during conditions of maximal flux through oxidative phosphorylation. Muscle acetylcarnitine, free-creatine, and lactate concentrations during recovery were unaffected by the HF diet. Taken together, our findings demonstrate that increasing muscle UCP3 expression does not diminish the rate of PCr resynthesis, allowing us to conclude that the primary role of UCP3 in humans is not uncoupling.
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UCP3 is upregulated under conditions of increased free fatty acid delivery to mitochondria, including fasting, insulin-deficient diabetes, and high-fat feeding.
“UCP3 is known to be upregulated under conditions of increased free fatty acid delivery to mitochondria, including fasting (44), insulin-deficient diabetes (134), and high-fat feeding (135).”
From Mitochondrial Dysfunction in Diabetes: From Molecular Mechanisms to Functional Significance and Therapeutic Opportunities (2010) · cites this paper as [135]
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Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961 · see citing works
Raza, H., John, A., & Nemmar, A. (2013) · Cellular Physiology and Biochemistry · doi:10.1159/000350087 · Cited by 47,311 · see citing works
A biometric study of basal metabolism in man
Harris, J. A., & Benedict, F. G. (2014) · Medical Entomology and Zoology · Cited by 1,847 · see citing works
The Gross Composition of the Body
Siri, W. E. (1956) · Advances in biological and medical physics · doi:10.1016/b978-1-4832-3110-5.50011-x · Cited by 1,771 · see citing works
Superoxide activates mitochondrial uncoupling proteins
Echtay, K. S., Roussel, D., St-Pierre, J., Jekabsons, M. B., Cadenas, S., Stuart, J. A., Harper, J. A., Roebuck, S. J., Morrison, A., Pickering, S., et al. (2002) · Nature · doi:10.1038/415096a · Cited by 1,382 · see citing works
Boss, O., Samec, S., Paoloni‐Giacobino, A., Rossier, C., Dulloo, A. G., Seydoux, J., Muzzin, P., & Giacobino, J. (1997) · FEBS Letters · doi:10.1016/s0014-5793(97)00384-0 · Cited by 1,041 · see citing works
Harris, R. C., Hultman, E., & Nordesjö, L. ‐. (1974) · Scandinavian Journal of Clinical and Laboratory Investigation · doi:10.3109/00365517409082477 · Cited by 857 · see citing works
Self-citationThe uncoupling protein homologues: UCP1, UCP2, UCP3, StUCP and AtUCP
Ricquier, D., & Bouillaud, F. (2000) · Biochemical Journal · doi:10.1042/bj3450161 · Cited by 806 · see citing works
Gong, D., He, Y., Karas, M., & Reitman, M. L. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.39.24129 · Cited by 740 · see citing works
Energy metabolism in uncoupling protein 3 gene knockout mice
Vidal-Puig, A. J., Grujic, D., Zhang, C.-Y., Hagen, T., Boss, O., Ido, Y., Szczepanik, A., Wade, J., Mootha, V., Cortright, R., et al. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m910179199 · Cited by 677 · see citing works
Mice overexpressing human uncoupling protein-3 in skeletal muscle are hyperphagic and lean
Clapham, J. C., Arch, J. R. S., Chapman, H., Haynes, A., Lister, C., Moore, G. B. T., Piercy, V., Carter, S. A., Lehner, I., Smith, S. A., et al. (2000) · Nature · doi:10.1038/35019082 · Cited by 604 · see citing works
Effect of oral creatine supplementation on skeletal muscle phosphocreatine resynthesis
Greenhaff, P. L., Bodin, K., Soderlund, K., & Hultman, E. (1994) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1994.266.5.e725 · Cited by 531 · see citing works
Self-citationThe time course of phosphorylcreatine resynthesis during recovery of the quadriceps muscle in man
Harris, R. C., Edwards, R. H. T., Hultman, E., Nordesj�, L. O., Nylind, B., & Sahlin, K. (1976) · Pflügers Archiv - European Journal of Physiology · doi:10.1007/bf00585149 · Cited by 527 · see citing works
Self-citationPecqueur, C., Alves‐Guerra, M., Gelly, C., Lévi-Meyrueis, C., Couplan, E., Collins, S., Ricquier, D., Bouillaud, F., & Miroux, B. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m006938200 · Cited by 474 · see citing works
Muscle Glycogen Synthesis after Exercise : an Enhancing Factor localized to the Muscle Cells in Man
BERGSTRÖM, J., & HULTMAN, E. (1966) · Nature · doi:10.1038/210309a0 · Cited by 473 · see citing works
Self-citationGong, D., Monemdjou, S., Gavrilova, O., Leon, L. R., Marcus‐Samuels, B., Chou, C. J., Everett, C. A., Kozak, L. P., Li, C., Deng, C., et al. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m910177199 · Cited by 385 · see citing works
Increased uncoupling protein-2 and -3 mRNA expression during fasting in obese and lean humans.
Millet, L., Vidal, H., Andréelli, F., Larrouy, D., Riou, J. P., Ricquier, D., Laville, M., & Langin, D. (1997) · Journal of Clinical Investigation · doi:10.1172/jci119811 · Cited by 351 · see citing works
Hamilton, J. A., & Kamp, F. (1999) · Diabetes · doi:10.2337/diabetes.48.12.2255 · Cited by 316 · see citing works
Harris, R. C., Hultman, E., & Nordesjö, L. O. (1974) · PubMed · Cited by 315 · see citing works
Self-citationDiet-induced changes in uncoupling proteins in obesity-prone and obesity-resistant strains of mice
Surwit, R. S., Wang, S., Petro, A. E., Sanchı́s, D., Raimbault, S., Ricquier, D., & Collins, S. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.7.4061 · Cited by 311 · see citing works
Walder, K. R. (1998) · Human Molecular Genetics · doi:10.1093/hmg/7.9.1431 · Cited by 293 · see citing works
Pattern and intensity of physical activity
Westerterp, K. R. (2001) · Nature · doi:10.1038/35069142 · Cited by 267 · see citing works
UCP2 and UCP3 rise in starved rat skeletal muscle but mitochondrial proton conductance is unchanged
Cadenas, S., Buckingham, J. A., Samec, S., Seydoux, J., Din, N., Dulloo, A. G., & Brand, M. D. (1999) · FEBS Letters · doi:10.1016/s0014-5793(99)01540-9 · Cited by 227 · see citing works
Bouchard, C., Përusse, L., Chagnon, Y. C., Warden, C. H., & Ricquier, D. (1997) · Human Molecular Genetics · doi:10.1093/hmg/6.11.1887 · Cited by 225 · see citing works
Argyropoulos, G. A., Brown, A. M., Willi, S. M., Zhu, J., He, Y., Reitman, M. L., Gevao, S. M., Spruill, I. J., & Garvey, W. T. (1998) · Journal of Clinical Investigation · doi:10.1172/jci4115 · Cited by 202 · see citing works
Cadenas, S., Echtay, K. S., Harper, J. A., Jekabsons, M. B., Buckingham, J. A., Grau, E., Abuin, A., Chapman, H., Clapham, J. C., & Brand, M. D. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m109736200 · Cited by 199 · see citing works
Radioisotopic assays of CoASH and carnitine and their acetylated forms in human skeletal muscle
Cederblad, G., Carlin, J. I., Constantin‐Teodosiu, D., Harper, P., & Hultman, E. (1990) · Analytical Biochemistry · doi:10.1016/0003-2697(90)90292-h · Cited by 173 · see citing works
Self-citationSCHRAUWEN, P., SARIS, W. H. M., & HESSELINK, M. K. C. (2001) · The FASEB Journal · doi:10.1096/fj.01-0400hyp · Cited by 172 · see citing works
Self-citationIn Vivo Effects of Uncoupling Protein-3 Gene Disruption on Mitochondrial Energy Metabolism
Cline, G. W., Vidal‐Puig, A., Dufour, S., Cadman, K. S., Lowell, B. B., & Shulman, G. I. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m102540200 · Cited by 142 · see citing works
Schrauwen, P., Xia, J., Bogardus, C., Pratley, R. E., & Ravussin, E. (1999) · Diabetes · doi:10.2337/diabetes.48.1.146 · Cited by 129 · see citing works
Self-citationHarper, M.-E., Dent, R., Monemdjou, S., Bézaire, V., Van Wyck, L., Wells, G., Kavaslar, G. N., Gauthier, A., Tesson, F., & McPherson, R. (2002) · Diabetes · doi:10.2337/diabetes.51.8.2459 · Cited by 129 · see citing works
Increased uncoupling proteins and decreased efficiency in palmitate-perfused hyperthyroid rat heart
Boehm, E. A., Jones, B. E., Radda, G. K., Veech, R. L., & Clarke, K. (2001) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.2001.280.3.h977 · Cited by 128 · see citing works
de Lange, P., Lanni, A., Beneduce, L., Moreno, M., Lombardi, A., Silvestri, E., & Goglia, F. (2001) · Endocrinology · doi:10.1210/endo.142.8.8303 · Cited by 125 · see citing works
A mitochondrial uncoupling artifact can be caused by expression of uncoupling protein 1 in yeast
Stuart, J. A., Harper, J. A., Brindle, K. M., Jekabsons, M. B., & Brand, M. D. (2001) · Biochemical Journal · doi:10.1042/0264-6021:3560779 · Cited by 116 · see citing works
Lanni, A., Beneduce, L., Lombardi, A., Moreno, M., Boss, O., Muzzin, P., Giacobino, J., & Goglia, F. (1999) · FEBS Letters · doi:10.1016/s0014-5793(99)00061-7 · Cited by 112 · see citing works
Zhang, C.-Y., Hagen, T., Mootha, V. K., Slieker, L. J., & Lowell, B. B. (1999) · FEBS Letters · doi:10.1016/s0014-5793(99)00441-x · Cited by 109 · see citing works
Ježek, P., Orosz, D. E., Modrianský, M., & Garlid, K. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)47176-1 · Cited by 108 · see citing works
Samec, S., Seydoux, J., & Dulloo, A. G. (1999) · Diabetes · doi:10.2337/diabetes.48.2.436 · Cited by 105 · see citing works
Assessment of mitochondrial energy coupling in vivo by 13 C/ 31 P NMR
Jucker, B. M., Dufour, S., Ren, J., Cao, X., Previs, S. F., Underhill, B., Cadman, K. S., & Shulman, G. I. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.120131997 · Cited by 99 · see citing works
Bézaire, V., Hofmann, W. E., Kramer, J. K. G., Kozak, L. P., & Harper, M.-E. (2001) · American Journal of Physiology. Endocrinology and Metabolism · doi:10.1152/ajpendo.2001.281.5.e975 · Cited by 90 · see citing works
Schrauwen, P., Hoppeler, H. H., Billeter, R., Bakker, A. H. F., & Pendergast, D. R. (2001) · International Journal of Obesity · doi:10.1038/sj.ijo.0801566 · Cited by 88 · see citing works
Self-citationProtein expression of UCP3 differs between human type 1, type 2a, and type 2b fibers
Hesselink, M. K. C., KEIZER, H. A., Borghouts, L. B., Schaart, G., Kornips, C. F. P., Slieker, L. J., Sloop, K. W., Saris, W. H. M., & Schrauwen, P. (2001) · The FASEB Journal · doi:10.1096/fj.00-0517fje · Cited by 87 · see citing works
Self-citationHARPER, J. A., STUART, J. A., JEKABSONS, M. B., ROUSSEL, D., BRINDLE, K. M., DICKINSON, K., JONES, R. B., & BRAND, M. D. (2002) · Biochemical Journal · doi:10.1042/bj3610049 · Cited by 87 · see citing works
Erickson‐Viitanen, S., Viitanen, P. V., Geiger, P. J., Yang, W. C., & Bessman, S. P. (1982) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)45394-5 · Cited by 84 · see citing works
Skeletal muscle UCP2 and UCP3 expression in trained and untrained male subjects
Schrauwen, P., Troost, F. J., Xia, J., Ravussin, É., & Saris, W. H. M. (1999) · International Journal of Obesity · doi:10.1038/sj.ijo.0801026 · Cited by 82 · see citing works
Self-citationRegulation of glycogenolysis in human skeletal muscle
Ren, J. M., & Hultman, E. (1989) · Journal of Applied Physiology · doi:10.1152/jappl.1989.67.6.2243 · Cited by 63 · see citing works
Self-citationJucker, B. M., Ren, J., Dufour, S., Cao, X., Previs, S. F., Cadman, K. S., & Shulman, G. I. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m007760200 · Cited by 51 · see citing works
Genetic and physiologic analysis of the role of uncoupling protein 3 in human energy homeostasis.
Chung, W. K., Luke, A., Cooper, R. S., Rotini, C., Vidal-Puig, A., Rosenbaum, M., Chua, M., Solanes, G., Zheng, M., Zhao, L., et al. (1999) · Diabetes · doi:10.2337/diabetes.48.9.1890 · Cited by 48 · see citing works
PDC activity and acetyl group accumulation in skeletal muscle during isometric contraction
Constantin‐Teodosiu, D., Cederblad, G., & Hultman, E. (1993) · Journal of Applied Physiology · doi:10.1152/jappl.1993.74.4.1712 · Cited by 37 · see citing works
Self-citationSöderlund, K., & Hultman, E. (1990) · Acta Physiologica Scandinavica · doi:10.1111/j.1748-1716.1990.tb08947.x · Cited by 26 · see citing works
Self-citationKariman, K., Chance, B., Burkhart, D., et al. (1986) · Brain Research · doi:10.1016/0006-8993(86)91307-7 · Cited by 14 · see citing works
Acetyl group delivery limits mitochondrial flux in human skeletal muscle
Campbell, S. P., Hultman, E., Teodosiu, D. C., et al. (2000) · Journal of Physiology (Cambridge) · Cited by 1 · see citing works
Self-citation
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- [16]Jucker, B.M., et al. 2000. Assessment of mitochondrial energy coupling in vivo by 13C/31P NMR. Proc. Natl. Acad. Sci. USA. 97 :6880-6884.
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- Authors
- Hesselink, M. K. C., Greenhaff, P. L., Constantin-Teodosiu, D., Hultman, E., Saris, W. H. M., Nieuwlaat, R., Schaart, G., Kornips, E., & Schrauwen, P.
- Affiliations
- Maastricht University (NL)
- Queen's Medical Centre (GB)
- Karolinska Institutet (SE)
- ORCID
- Venue
- Journal of Clinical Investigation
- Published
- 2003-02-15
- Volume
- 111
- Issue
- 4
- Pages
- 479-486
- ISSN
- 0021-9738 (ISSN-L)
- PMC
- PMC152374
- OpenAlex
- W2130962964
- Cited by
- 82
- References
- 52
- Citations by year
- 2024: 1 · 2023: 1 · 2022: 2 · 2021: 2 · 2019: 1 · 2018: 1 · 2016: 2 · 2015: 1 · 2014: 3 · 2013: 2 · 2012: 3
- FWCI
- 4.60
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Clinical Investigation (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Research Publications (Maastricht University) (publishedVersion, other-oa)
- Research Publications (Maastricht University) (submittedVersion, other-oa)
- Funders
- Eli Lilly and Company
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Health Sciences › Medicine › Complementary and alternative medicine › Cardiovascular and exercise physiology
- Keywords
- UCP3, mitochondrial uncoupling, skeletal muscle, phosphocreatine resynthesis, high-fat diet, mitochondrial coupling, oxidative phosphorylation, creatine kinase, acetyl-L-carnitine, lactate, post-exercise recovery
- MeSH terms
- Uncoupling Protein 3, Adult, Carrier Proteins, Creatine, Dietary Fats, Fatty Acids, Proton-Translocating ATPases, Humans, Ion Channels, Male, Mitochondria, Muscle, Muscle Contraction, Oxidative Phosphorylation, Phosphocreatine, Uncoupling Agents, Muscle, Skeletal, Lactic Acid, Mitochondrial Proteins
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