Cited by 57Genomics and Phylogenetic StudiesHuman-Animal Interaction StudiesGenetic and phenotypic traits in livestock

Sequence comparison of prefrontal cortical brain transcriptome from a tame and an aggressive silver fox (Vulpes vulpes)

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Kukekova, Anna V, Johnson, Jennifer L, Teiling, Clotilde, Li, Lewyn, Oskina, Irina N, Kharlamova, Anastasiya V, Gulevich, Rimma G, Padte, Ravee +9 more·2011·BMC Genomics

Abstract

BACKGROUND: Two strains of the silver fox (Vulpes vulpes), with markedly different behavioral phenotypes, have been developed by long-term selection for behavior. Foxes from the tame strain exhibit friendly behavior towards humans, paralleling the sociability of canine puppies, whereas foxes from the aggressive strain are defensive and exhibit aggression to humans. To understand the genetic differences underlying these behavioral phenotypes fox-specific genomic resources are needed. RESULTS: cDNA from mRNA from pre-frontal cortex of a tame and an aggressive fox was sequenced using the Roche 454 FLX Titanium platform (> 2.5 million reads & 0.9 Gbase of tame fox sequence; >3.3 million reads & 1.2 Gbase of aggressive fox sequence). Over 80% of the fox reads were assembled into contigs. Mapping fox reads against the fox transcriptome assembly and the dog genome identified over 30,000 high confidence fox-specific SNPs. Fox transcripts for approximately 14,000 genes were identified using SwissProt and the dog RefSeq databases. An at least 2-fold expression difference between the two samples (p < 0.05) was observed for 335 genes, fewer than 3% of the total number of genes identified in the fox transcriptome. CONCLUSIONS: Transcriptome sequencing significantly expanded genomic resources available for the fox, a species without a sequenced genome. In a very cost efficient manner this yielded a large number of fox-specific SNP markers for genetic studies and provided significant insights into the gene expression profile of the fox pre-frontal cortex; expression differences between the two fox samples; and a catalogue of potentially important gene-specific sequence variants. This result demonstrates the utility of this approach for developing genomic resources in species with limited genomic information.

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  • A catalogue of gene-specific sequence variants was detected as differentially expressed between a strain of silver fox selected for aggressive behaviors and its tame counterpart.

    “Moreover, a catalogue of gene-specific sequence variants was detected as differentially expressed between a strain of silver fox selected for aggressive behaviors when compared to its tame counterpart [24].”
  • Kukekova et al. [24] compared PFC expression between aggressive and docile strains of silver fox and observed enrichment of pathways related to cellular movement, growth and proliferation, hematological system development and antigen presentation.

    “Similar results were obtained by Kukekova et al. [24]; they compared the PFC expression between aggressive and docile strains of silver fox and also observed an enrichment of pathways related to cellular movement, growth and proliferation, hematological system development and antigen presentation.”

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Research works printed but not linked: 2

  1. Macdonald DW Reynolds JC Sillero-Zubiri C, Hoffmann M, Macdonald DW Red Fox Canids: Foxes, Wolves, Jackals and Dogs 2004 Cambridge, IUCN 129 136
  2. NCBI http://www.ncbi.nlm.nih.gov/nuccore/limits

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Authors
Kukekova, A. V., Johnson, J. L., Teiling, C., Li, L., Oskina, I. N., Kharlamova, A. V., Gulevich, R. G., Padte, R., Dubreuil, M. M., Vladimirova, A. V., Shepeleva, D. V., Shikhevich, S. G., Sun, Q., Ponnala, L., Temnykh, S. V., Trut, L. N., & Acland, G. M.
Affiliations
  • Cornell University (US)
  • Br4Science (Brazil) (BR)
  • Russian Academy of Sciences (RU)
Corresponding author
Kukekova, Anna V
Venue
BMC Genomics
Published
2011-10-03
Volume
12
Issue
1
Pages
482-482
ISSN
1471-2164 (ISSN-L)
OpenAlex
W2098452955
Cited by
57
References
73
Citations by year
2026: 1 · 2025: 2 · 2023: 2 · 2022: 7 · 2021: 5 · 2020: 5 · 2019: 2 · 2018: 6 · 2017: 5 · 2016: 4 · 2015: 6 · 2014: 2 · 2013: 6 · 2012: 3
FWCI
1.56
Type
journal article
Language
en
Open access
Locations
  • BMC Genomics (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
  • Foundation Fighting Blindness
  • National Institutes of Health
  • Russian Academy of Sciences
  • National Institute of Mental Health (MH077811, R01MH077811)
  • National Eye Institute (EY-006855)
  • National Institute of General Medical Sciences (GM082910, R24GM082910)
  • Fogarty International Center (R03TW008098)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Human-Animal Interaction Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and phenotypic traits in livestock
Keywords
silver fox, red fox, prefrontal cortex, transcriptome, behavioral phenotypes, gene expression profiling, genomic resources
MeSH terms
Animals, Dogs, Foxes, Databases, Factual, Prefrontal Cortex, Sequence Analysis, DNA, Contig Mapping, Polymorphism, Single Nucleotide, Transcriptome

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