CoChat
Sign Up
Cited by 300Ruminant Nutrition and Digestive PhysiologyFatty Acid Research and HealthMeat and Animal Product Quality

The DOI matches its Crossref record; 49 of the 50 references it cites were found in a registry or an index.

Toxicity of unsaturated fatty acids to the biohydrogenating ruminal bacterium, Butyrivibrio fibrisolvens

Open access
Maia, Margarida RG, Chaudhary, Lal C, Bestwick, Charles S, Richardson, Anthony J, McKain, Nest, Larson, Tony R, Graham, Ian A, Wallace, Robert J·2010·BMC Microbiology
DOI 10.1186/1471-2180-10-52PMC PMC2836310
Open this paper in CoChat — free accountOpen PDF

Abstract

BACKGROUND: Health-promoting polyunsaturated fatty acids (PUFA) are abundant in forages grazed by ruminants and in vegetable and fish oils used as dietary supplements, but only a small proportion of PUFA finds its way into meat and milk, because of biohydrogenation in the rumen. Butyrivibrio fibrisolvens plays a major role in this activity. The aim of this study was to investigate the mechanisms by which PUFA affect the growth of B. fibrisolvens, how PUFA are metabolized and the metabolic response to growth in the presence of PUFA. RESULTS: Linoleic acid (LA; cis-9, cis-12-18:2) and alpha-linolenic acid (LNA; cis-9, cis-12, cis-15-18:3) increased the lag phase of B. fibrisolvens JW11, LNA having the greater effect. Growth was initiated only when the PUFA had been converted to vaccenic acid (VA; trans-11-18:1). The major fish oil fatty acids, eicosapentaenoic acid (EPA; 20:5(n-3)) and docosahexaenoic acid (DHA; 22:6(n-3)), were not metabolized and prevented growth. Cellular integrity, as determined fluorimetrically by propidium iodide (PI) ingression, was affected as much by 18:1 fatty acids, including VA, as 18:2 fatty acids. The methyl esters of LNA, LA, EPA and DHA had no effect on growth or other measurements. The ATP pool decreased by 2/3 when LA was added to growing bacteria, whereas most acyl CoA pools decreased by >96%. CONCLUSIONS: It was concluded that biohydrogenation occurs to enable B. fibrisolvens to survive the bacteriostatic effects of PUFA, and that the toxicity of PUFA is probably mediated via a metabolic effect rather than disruption of membrane integrity.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1186/1471-2180-10-52

Cites

49 of 49 references verified0 citation errors · 0 not checkable

Statistics

Figures

Figure 1 Concentration of fatty acids in the medium following inoculation of B. fibrisolvens JW11 into M2 medium containing 50 μg ml-1 linoleic acid (LA; cis-9, cis-12-18:2). Growth (open circle, OD650), LA (square), cis-9, trans-11-18:2 (black circle), trans-11-18:1 (triangle). Results are means and SD from three cultures.
Figure 1 Concentration of fatty acids in the medium following inoculation of B. fibrisolvens JW11 into M2 medium containing 50 μg ml-1 linoleic acid (LA; cis-9, cis-12-18:2). Growth (open circle, OD650), LA (square), cis-9, trans-11-18:2 (black circle), trans-11-18:1 (triangle). Results are means and SD from three cultures.CC BY · PubMed Central
Figure 2 Concentration of fatty acids in the medium following inoculation of B. fibrisolvens JW11 into M2 medium containing 50 μg ml-1 α-linolenic acid (LNA; cis-9, cis-12, cis-15-18:3). Growth (open circle, OD650), LNA (black circle), cis-9, trans-11, cis-15-18:3 (open square), trans-9, trans-11, cis-15-18:3 (black square), trans-11, cis-15-18:2 (open triangle), cis-9, trans-11-18:2 (black triangle), trans-11-18:1 (diamond). Results are means and SD from three cultures.
Figure 2 Concentration of fatty acids in the medium following inoculation of B. fibrisolvens JW11 into M2 medium containing 50 μg ml-1 α-linolenic acid (LNA; cis-9, cis-12, cis-15-18:3). Growth (open circle, OD650), LNA (black circle), cis-9, trans-11, cis-15-18:3 (open square), trans-9, trans-11, cis-15-18:3 (black square), trans-11, cis-15-18:2 (open triangle), cis-9, trans-11-18:2 (black triangle), trans-11-18:1 (diamond). Results are means and SD from three cultures.CC BY · PubMed Central
Figure 3 Influence of different fatty acids and fatty acid methyl esters on cell integrity of B. fibrisolvens JW11. Loss of cell integrity was determined fluorimetrically by propidium iodide fluorescence. LNA, cis-9, cis-12, cis-15-18:3; γLNA, cis-6, cis-9, cis-12-18:3; LA, cis-9, cis-12-18:2; CLA, a mixture of cis-9, trans-11-18:2 and trans-10, cis-12-18:2; VA, trans-11-18:1; OA, cis-9-18:1; SA, 18:0. In order of increasing shading density: 50 μg fatty acid ml-1, 200 μg fatty acid ml-1, 50 μg fatty acid methyl ester ml-1, 200 μg fatty acid methyl ester ml-1. Results are means and SD from three determinations.
Figure 3 Influence of different fatty acids and fatty acid methyl esters on cell integrity of B. fibrisolvens JW11. Loss of cell integrity was determined fluorimetrically by propidium iodide fluorescence. LNA, cis-9, cis-12, cis-15-18:3; γLNA, cis-6, cis-9, cis-12-18:3; LA, cis-9, cis-12-18:2; CLA, a mixture of cis-9, trans-11-18:2 and trans-10, cis-12-18:2; VA, trans-11-18:1; OA, cis-9-18:1; SA, 18:0. In order of increasing shading density: 50 μg fatty acid ml-1, 200 μg fatty acid ml-1, 50 μg fatty acid methyl ester ml-1, 200 μg fatty acid methyl ester ml-1. Results are means and SD from three determinations.CC BY · PubMed Central
Figure 4 Influence of different fatty acids on PI fluorescence of B. fibrisolvens JW11 by flow cytometry. Black - live cells; green - heat-killed cells; pink - 50 μg ml-1 LA; turquoise - 50 μg ml-1 LNA; orange - 50 μg ml-1 CLA; blue - 50 μg ml-1 VA; yellow - 50 μg ml-1 SA.
Figure 4 Influence of different fatty acids on PI fluorescence of B. fibrisolvens JW11 by flow cytometry. Black - live cells; green - heat-killed cells; pink - 50 μg ml-1 LA; turquoise - 50 μg ml-1 LNA; orange - 50 μg ml-1 CLA; blue - 50 μg ml-1 VA; yellow - 50 μg ml-1 SA.CC BY · PubMed Central
Figure 5 Influence of sodium lactate (70 mM) on the loss of cell integrity of B. fibrisolvens JW11 following incubation with LA (50 μg ml-1). Loss of cell integrity was determined by fluorescence in the presence of propidium iodide. Sodium lactate + LA (open bar), LA alone (black bar). Results are means and SD from three cultures, each of which was subject to 8 replicate measurements (n = 24).
Figure 5 Influence of sodium lactate (70 mM) on the loss of cell integrity of B. fibrisolvens JW11 following incubation with LA (50 μg ml-1). Loss of cell integrity was determined by fluorescence in the presence of propidium iodide. Sodium lactate + LA (open bar), LA alone (black bar). Results are means and SD from three cultures, each of which was subject to 8 replicate measurements (n = 24).CC BY · PubMed Central
Figure 6 Influence of LA on ATP pools of B. fibrisolvens JW11 after 50% inoculation into fresh medium. LA (black circle), no LA (open circle). Results are means and SD from three separate cultures.
Figure 6 Influence of LA on ATP pools of B. fibrisolvens JW11 after 50% inoculation into fresh medium. LA (black circle), no LA (open circle). Results are means and SD from three separate cultures.CC BY · PubMed Central

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Show all 50 references

Research works printed but not linked: 3

  1. Hungate RE: A roll tube method for cultivation of strict anaerobes. Methods in Microbiology. Edited by: Norris JR, Ribbons DW. 1969, London: Academic Press, 3B: 117-132. full_text.
  2. Hobson PN: Rumen bacteria. Methods in Microbiology. Edited by: Norris JR, Ribbons DW. 1969, London: Academic Press, 3B: 133-139. full_text.
  3. Herbert D, Phipps PJ, Strange RE: Chemical analysis of microbial cells. Methods in Microbiology. Edited by: Norris JR, Ribbons DW. 1971, London: Academic Press, 5B: 209-344. full_text.

Cited by

Sorted by citations

See all 300

Related

Metadata

Authors
Maia, M. R., Chaudhary, L. C., Bestwick, C. S., Richardson, A. J., McKain, N., Larson, T. R., Graham, I. A., & Wallace, R. J.
Affiliations
  • University of Aberdeen (GB)
  • University of York (GB)
Corresponding author
Wallace, Robert J
ORCID
Venue
BMC Microbiology
Publisher
Springer Science and Business Media LLC
Published
2010-02-18
Volume
10
Issue
1
Pages
52-52
ISSN
1471-2180 (ISSN-L)
DOI
10.1186/1471-2180-10-52
PMC
PMC2836310
OpenAlex
W2162688963
Cited by
298
References
50
Citations by year
2026: 16 · 2025: 16 · 2024: 16 · 2023: 25 · 2022: 21 · 2021: 25 · 2020: 22 · 2019: 21 · 2018: 20 · 2017: 18 · 2016: 21 · 2015: 20 · 2014: 22 · 2013: 11 · 2012: 13
FWCI
23.75
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • BMC Microbiology (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Aberdeen University Research Archive (Aberdeen University) (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Scottish Government
  • Fundação para a Ciência e a Tecnologia
Topics
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Ruminant Nutrition and Digestive Physiology
  • Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Meat and Animal Product Quality
Keywords
polyunsaturated fatty acids, Butyrivibrio fibrisolvens, biohydrogenation, rumen bacteria, linoleic acid, alpha-linolenic acid, vaccenic acid, eicosapentaenoic acid, docosahexaenoic acid, fish oil, bacterial growth inhibition, membrane integrity, ATP depletion, fatty acid metabolism
MeSH terms
Acyl Coenzyme A, Adenosine Triphosphate, Animals, Cell Membrane, Culture Media, Fatty Acids, Unsaturated, Flow Cytometry, Hydrogenation, Sheep, Sodium Lactate, Linoleic Acid, Butyrivibrio, Lipid Metabolism

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review