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Cited by 16Neurogenesis and neuroplasticity mechanismsNeuroscience and Neural EngineeringCellular Mechanics and Interactions

A neural extracellular matrix-based method for in vitrohippocampal neuron culture and dopaminergic differentiation of neural stem cells

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García-Parra, Patricia, Maroto, Marcos, Cavaliere, Fabio, Naldaiz-Gastesi, Neia, Álava, José Iñaki, García, Antonio G, López de Munain, Adolfo, Izeta, Ander·2013·BMC Neuroscience
DOI 10.1186/1471-2202-14-48PMC PMC3639926
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Abstract

BACKGROUND: The ability to recreate an optimal cellular microenvironment is critical to understand neuronal behavior and functionality in vitro. An organized neural extracellular matrix (nECM) promotes neural cell adhesion, proliferation and differentiation. Here, we expanded previous observations on the ability of nECM to support in vitro neuronal differentiation, with the following goals: (i) to recreate complex neuronal networks of embryonic rat hippocampal cells, and (ii) to achieve improved levels of dopaminergic differentiation of subventricular zone (SVZ) neural progenitor cells. METHODS: Hippocampal cells from E18 rat embryos were seeded on PLL- and nECM-coated substrates. Neurosphere cultures were prepared from the SVZ of P4-P7 rat pups, and differentiation of neurospheres assayed on PLL- and nECM-coated substrates. RESULTS: When seeded on nECM-coated substrates, both hippocampal cells and SVZ progenitor cells showed neural expression patterns that were similar to their poly-L-lysine-seeded counterparts. However, nECM-based cultures of both hippocampal neurons and SVZ progenitor cells could be maintained for longer times as compared to poly-L-lysine-based cultures. As a result, nECM-based cultures gave rise to a more branched neurite arborization of hippocampal neurons. Interestingly, the prolonged differentiation time of SVZ progenitor cells in nECM allowed us to obtain a purer population of dopaminergic neurons. CONCLUSIONS: We conclude that nECM-based coating is an efficient substrate to culture neural cells at different stages of differentiation. In addition, neural ECM-coated substrates increased neuronal survival and neuronal differentiation efficiency as compared to cationic polymers such as poly-L-lysine.

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  • The extracellular matrix has been shown to influence adhesion, proliferation, mobility, and morphology of rather robust cell lines.

    “However, the extracellular matrix has also been shown to influence adhesion, proliferation, mobility, and morphology of rather robust cell lines (Fiegel et al., 2004; García-Parra et al., 2013; Liberio et al., 2014).”

    From In Vitro research Reproducibility: Keeping up High Standards (2019) · cites this paper as García-Parra et al., 2013

    The Background says ECM promotes adhesion and proliferation and that neural ECM facilitates cell movement and neurite extension. The Results and discussion report greater neurite sprouting on neural ECM, supporting an effect on neuronal morphology. But the study examines primary hippocampal cells and neural progenitors, not the claimed effects across robust cell lines. (LLM, conf=0.85)

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Cited by

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Authors
García-Parra, P., Maroto, M., Cavaliere, F., Naldaiz-Gastesi, N., Álava, J. I., García, A. G., López de Munain, A., & Izeta, A.
Affiliations
  • Biogipuzkoa Health Research Institute (ES)
  • Hospital Universitario de La Princesa (ES)
  • University of the Basque Country (ES)
  • Basque Culinary Center (ES)
Corresponding author
García-Parra, Patricia
ORCID
Venue
BMC Neuroscience
Publisher
Springer Science and Business Media LLC
Published
2013-04-18
Volume
14
Issue
1
Pages
48-48
ISSN
1471-2202 (ISSN-L)
DOI
10.1186/1471-2202-14-48
PMC
PMC3639926
OpenAlex
W2071828817
Cited by
16
References
3
Citations by year
2024: 2 · 2022: 1 · 2021: 1 · 2020: 2 · 2019: 2 · 2018: 1 · 2016: 1 · 2015: 1 · 2014: 2 · 2013: 3
FWCI
0.87
Type
journal article
Language
en
Open access
Licence
Locations
  • BMC Neuroscience (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Communities in ADDI (Universidad del Pais Vasco) (submittedVersion, other-oa)
  • Communities in ADDI (University of the Basque Country) (submittedVersion, cc-by)
  • LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (submittedVersion, other-oa)
  • Hispana (submittedVersion, cc-by)
Funders
  • Fundación Centros Tecnológicos Iñaki Goenaga
  • Eusko Jaurlaritza
  • Ministerio de Economía y Competitividad (21795, SAF2010-21795)
  • Ministerio de Ciencia e Innovación (SAF2010-21795, RETICS-RD06/0026)
  • Fundação para a Ciência e a Tecnologia
  • Instituto de Salud Carlos III (CES09/015, RETICS-RD06/0026, RD 06/0026, PI10/02871, I3SNS)
Topics
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neural Engineering
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
Keywords
dopaminergic differentiation, neural stem cells, neurospheres, substrate coating, poly-L-lysine, neurite arborization, neuronal survival, neuronal differentiation, rat embryo
MeSH terms
Animals, Animals, Newborn, Cell Differentiation, Cell Survival, Cells, Cultured, Cerebral Ventricles, Chondroitin Sulfates, Dose-Response Relationship, Drug, Embryo, Mammalian, Enzyme Inhibitors, Extracellular Matrix, Female, Hippocampus, Male, Microscopy, Electron, Scanning, Nerve Tissue Proteins, Oligomycins, Pregnancy, Rotenone, Tyrosine 3-Monooxygenase, Amyloid beta-Peptides, Rats, Sprague-Dawley, Microscopy, Confocal, Rats, Neural Stem Cells, Dopaminergic Neurons, In Vitro Techniques

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