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Dynamic regulation of genome-wide pre-mRNA splicing and stress tolerance by the Sm-like protein LSm5 in Arabidopsis

Open access
Cui, Peng, Zhang, Shoudong, Ding, Feng, Ali, Shahjahan, Xiong, Liming·2014·Genome Biology
DOI 10.1186/gb-2014-15-1-r1
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Abstract

Background: Sm-like proteins are highly conserved proteins that form the core of the U6 ribonucleoprotein and function in several mRNA metabolism processes, including pre-mRNA splicing. Despite their wide occurrence in all eukaryotes, little is known about the roles of Sm-like proteins in the regulation of splicing. Results: Here, through comprehensive transcriptome analyses, we demonstrate that depletion of the Arabidopsis supersensitive to abscisic acid and drought 1 gene ( SAD1 ), which encodes Sm-like protein 5 (LSm5), promotes an inaccurate selection of splice sites that leads to a genome-wide increase in alternative splicing. In contrast, overexpression of SAD1 strengthens the precision of splice-site recognition and globally inhibits alternative splicing. Further, SAD1 modulates the splicing of stress-responsive genes, particularly under salt-stress conditions. Finally, we find that overexpression of SAD1 in Arabidopsis improves salt tolerance in transgenic plants, which correlates with an increase in splicing accuracy and efficiency for stress-responsive genes. Conclusions: We conclude that SAD1 dynamically controls splicing efficiency and splice-site recognition in Arabidopsis , and propose that this may contribute to SAD1 -mediated stress tolerance through the metabolism of transcripts expressed from stress-responsive genes. Our study not only provides novel insights into the function of Sm-like proteins in splicing, but also uncovers new means to improve splicing efficiency and to enhance stress tolerance in a higher eukaryote.

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Metadata

Authors
Cui, P., Zhang, S., Ding, F., Ali, S., & Xiong, L.
Venue
Genome Biology
Publisher
Springer Science and Business Media LLC
Published
2014-01-07
Volume
15
Issue
1
Pages
R1-R1
ISSN
  • 1465-6906 (ISSN-L)
  • 1474-760X
DOI
10.1186/gb-2014-15-1-r1
OpenAlex
W1970707135
Cited by
2,294
References
3
FWCI
16.31
Type
journal article
Language
en
Open access
gold
Licence

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