Lactobacillus crispatus inhibits growth of Gardnerella vaginalis and Neisseria gonorrhoeae on a porcine vaginal mucosa model

Breshears, Laura M.; Edwards, Vonetta L.; Ravel, Jacques; Peterson, Marnie L.

doi:10.1186/s12866-015-0608-0

Cited by 140Reproductive tract infections researchProbiotics and Fermented FoodsGut microbiota and health

Lactobacillus crispatus inhibits growth of Gardnerella vaginalis and Neisseria gonorrhoeae on a porcine vaginal mucosa model

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Breshears, Laura M., Edwards, Vonetta L., Ravel, Jacques, Peterson, Marnie L.·2015·BMC Microbiology

DOI 10.1186/s12866-015-0608-0PMC PMC4675025

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Abstract

BACKGROUND: The vaginal microbiota can impact the susceptibility of women to bacterial vaginosis (BV) and sexually transmitted infections (STIs). BV is characterized by depletion of Lactobacillus spp., an overgrowth of anaerobes (often dominated by Gardnerella vaginalis) and a pH > 4.5. BV is associated with an increased risk of acquiring STIs such as chlamydia and gonorrhea. While these associations have been identified, the molecular mechanism(s) driving the risk of infections are unknown. An ex vivo porcine vaginal mucosal model (PVM) was developed to explore the mechanistic role of Lactobacillus spp. in affecting colonization by G. vaginalis and Neisseria gonorrhoeae. RESULTS: The data presented here demonstrate that all organisms tested can colonize and grow on PVM to clinically relevant densities. Additionally, G. vaginalis and N. gonorrhoeae form biofilms on PVM. It was observed that lactic acid, acetic acid, and hydrochloric acid inhibit the growth of G. vaginalis on PVM in a pH-dependent manner. N. gonorrhoeae grows best in the presence of lactic acid at pH 5.5, but did not grow well at this pH in the presence of acetic acid. Finally, a clinical Lactobacillus crispatus isolate (24-9-7) produces lactic acid and inhibits growth of both G. vaginalis and N. gonorrhoeae on PVM. CONCLUSIONS: These data reveal differences in the effects of pH, various acids and L. crispatus on the growth of G. vaginalis and N. gonorrhoeae on a live vaginal mucosal surface. The PVM is a useful model for studying the interactions of commensal vaginal microbes with pathogens and the mechanisms of biofilm formation on the vaginal mucosa.

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Vaginal Lactobacillus spp. limit colonization of BV-associated microbes and are protective against deleterious viral effects through maintenance of low pH and bacteriocin production.
“This is significant because it then follows that vaginal Lactobacillus spp. (sensu lato) limit colonization of BV-associated microbes and thus by extension are protective against the aforementioned deleterious viral effects through maintenance of a low pH (Breshears et al., 2015), and bacteriocin production (Stoyancheva et al., 2014).”
From Viral Infections, the Microbiome, and Probiotics (2021) · cites this paper as Breshears et al., 2015

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Non-cultural detection of Neisseria gonorrhoeae in cervical and vaginal washings
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Use of porcine vaginal tissue ex-vivo to model environmental effects on vaginal mucosa to toxic shock syndrome toxin-1
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Vaginal microbiota in healthy women and patients with bacterial vaginosis and nonspecific vaginitis
Aleshkin, V. A., Александровна, В. Е., & Ba, S. (2006) · Microbial Ecology in Health and Disease · doi:10.3402/mehd.v18i2.7694 · Cited by 13 · see citing works
Vaginal microbiota in healthy women and patients with bacterial vaginosis and nonspecific vaginitis
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Vaginal microbiota and the potential of Lactobacillus derivatives in maintaining vaginal health
Chee, W. J. Y., Chew, S. Y., & Than, L. T. L. (2020) · Microbial Cell Factories · doi:10.1186/s12934-020-01464-4 · Cited by 709 · see citing works
The role of lactic acid production by probiotic Lactobacillus species in vaginal health
Tachedjian, G., Aldunate, M., Bradshaw, C. S., & Cone, R. A. (2017) · Research in Microbiology · doi:10.1016/j.resmic.2017.04.001 · Cited by 557 · see citing works
The vaginal microbiota, human papillomavirus infection and cervical intraepithelial neoplasia: What do we know and where are we going next
Mitra, A., MacIntyre, D. A., Marchesi, J. R., Lee, Y. S., Bennett, P. R., & Kyrgiou, M. (2016) · Microbiome · doi:10.1186/s40168-016-0203-0 · Cited by 484 · see citing works
The vaginal microbiota associates with the regression of untreated cervical intraepithelial neoplasia 2 lesions
Mitra, A., MacIntyre, D. A., Ntritsos, G., Smith, A., Tsilidis, K. K., Marchesi, J. R., Bennett, P. R., Moscicki, A., & Kyrgiou, M. (2020) · Nature Communications · doi:10.1038/s41467-020-15856-y · Cited by 258 · see citing works
Options and Limitations in Clinical Investigation of Bacterial Biofilms
Magana, M., Sereti, C., Ioannidis, A., Mitchell, C. A., Ball, A. R., Magiorkinis, E., Chatzipanagiotou, S., Hamblin, M. R., Hadjifrangiskou, M., & Tegos, G. P. (2018) · Clinical Microbiology Reviews · doi:10.1128/cmr.00084-16 · Cited by 243 · see citing works
Cervicovaginal microbiota, women's health, and reproductive outcomes
Kroon, S. J., Ravel, J., & Huston, W. M. (2018) · Fertility and Sterility · doi:10.1016/j.fertnstert.2018.06.036 · Cited by 235 · see citing works
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Viral Infections, the Microbiome, and Probiotics
Harper, A., Vijayakumar, V., Ouwehand, A. C., ter Haar, J., Obis, D., Espadaler, J., Binda, S., Desiraju, S., & Day, R. (2021) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2020.596166 · Cited by 177 · see citing works
Probiotics or pro‐healers: the role of beneficial bacteria in tissue repair
Lukić, J., Chen, V., Strahinić, I., Begović, J., Lev‐Tov, H. A., Davis, S. C., Tomic‐Canic, M., & Pastar, I. (2017) · Wound Repair and Regeneration · doi:10.1111/wrr.12607 · Cited by 175 · see citing works
Risk factors for human papillomavirus infection, cervical intraepithelial neoplasia and cervical cancer: an umbrella review and follow-up Mendelian randomisation studies
Bowden, S. J., Doulgeraki, T., Bouras, E., Markozannes, G., Athanasiou, A., Grout-Smith, H., Kechagias, K. S., Ellis, L. B., Zuber, V., Chadeau‐Hyam, M., et al. (2023) · BMC Medicine · doi:10.1186/s12916-023-02965-w · Cited by 196 · see citing works
Effects of combined oral contraceptives, depot medroxyprogesterone acetate and the levonorgestrel-releasing intrauterine system on the vaginal microbiome
Brooks, J. P., Edwards, D., Blithe, D., Fettweis, J. M., Serrano, M. G., Sheth, N. U., Strauss, J. F., Buck, G. A., & Jefferson, K. K. (2016) · Contraception · doi:10.1016/j.contraception.2016.11.006 · Cited by 128 · see citing works
The Anti-Inflammatory and Curative Exponent of Probiotics: A Comprehensive and Authentic Ingredient for the Sustained Functioning of Major Human Organs
Virk, M. S., Virk, M. A., He, Y., Tufail, T., Gul, M., Qayum, A., Rehman, A., Rashid, A., Ekumah, J., Han, X., et al. (2024) · Nutrients · doi:10.3390/nu16040546 · Cited by 137 · see citing works
The implausible “in vivo” role of hydrogen peroxide as an antimicrobial factor produced by vaginal microbiota
Tachedjian, G., O’Hanlon, D. E., & Ravel, J. (2018) · Microbiome · doi:10.1186/s40168-018-0418-3 · Cited by 125 · see citing works
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Etiology of bacterial vaginosis and polymicrobial biofilm formation
Jung, H., Ehlers, M. M., Lombaard, H. A., Redelinghuys, M. J., & Kock, M. M. (2017) · Critical Reviews in Microbiology · doi:10.1080/1040841x.2017.1291579 · Cited by 127 · see citing works
Progress Toward a Gonococcal Vaccine: The Way Forward
Russell, M. W., Jerse, A. E., & Gray‐Owen, S. D. (2019) · Frontiers in Immunology · doi:10.3389/fimmu.2019.02417 · Cited by 85 · see citing works
The Complex Link between the Female Genital Microbiota, Genital Infections, and Inflammation
Dabee, S., Passmore, J.-A. S., Heffron, R., & Jaspan, H. B. (2021) · Infection and Immunity · doi:10.1128/iai.00487-20 · Cited by 89 · see citing works
Vaginal Lactobacilli Reduce Neisseria gonorrhoeae Viability through Multiple Strategies: An in Vitro Study
Foschi, C., Salvo, M., Cevenini, R., Parolin, C., Vitali, B., & Marangoni, A. (2017) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2017.00502 · Cited by 86 · see citing works
The potential of lactic acid bacteria to colonize biotic and abiotic surfaces and the investigation of their interactions and mechanisms
Arena, M. P., Capozzi, V., Spano, G., & Fiocco, D. (2017) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-017-8182-z · Cited by 86 · see citing works
Colonization of the cervicovaginal space with Gardnerella vaginalis leads to local inflammation and cervical remodeling in pregnant mice
Sierra, L., Brown, A. G., Barila, G., Anton, L., Barnum, C. E., Shetye, S. S., Soslowsky, L. J., & Elovitz, M. A. (2018) · PLoS ONE · doi:10.1371/journal.pone.0191524 · Cited by 77 · see citing works
Vaginal Lactobacillus Inhibits HIV-1 Replication in Human Tissues Ex Vivo
Palomino, R. A. Ñ., Zicari, S., Vanpouille, C., Vitali, B., & Margolis, L. B. (2017) · Frontiers in Microbiology · doi:10.3389/fmicb.2017.00906 · Cited by 77 · see citing works
Gardnerella and vaginal health: the truth is out there
Rosca, A. S., Castro, J., Sousa, L. G. V., & Cerca, N. (2019) · FEMS Microbiology Reviews · doi:10.1093/femsre/fuz027 · Cited by 78 · see citing works
Diverse Expression of Antimicrobial Activities Against Bacterial Vaginosis and Urinary Tract Infection Pathogens by Cervicovaginal Microbiota Strains of Lactobacillus gasseri and Lactobacillus crispatus
Atassi, F., Pho Viet Ahn, D. L., & Lievin-Le Moal, V. (2019) · Frontiers in Microbiology · doi:10.3389/fmicb.2019.02900 · Cited by 71 · see citing works
In vivo microbiome and associated immune markers: New insights into the pathogenesis of vaginal dysbiosis
Campisciano, G., Zanotta, N., Licastro, D., Seta, F. D., & Comar, M. (2018) · Scientific Reports · doi:10.1038/s41598-018-20649-x · Cited by 66 · see citing works
Investigation of auranofin and gold-containing analogues antibacterial activity against multidrug-resistant Neisseria gonorrhoeae
Elkashif, A., & Seleem, M. N. (2020) · Scientific Reports · doi:10.1038/s41598-020-62696-3 · Cited by 64 · see citing works
The Effect of Gender-Affirming Medical Care on the Vaginal and Neovaginal Microbiomes of Transgender and Gender-Diverse People
Krakowsky, Y., Potter, E., Hallarn, J., Monari, B., Wilcox, H., Bauer, G. R., Ravel, J., & Prodger, J. L. (2022) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2021.769950 · Cited by 59 · see citing works
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Does Lactobacillus Exert a Protective Effect on the Development of Cervical and Endometrial Cancer in Women?
Frąszczak, K., Barczyński, B., & Kondracka, A. (2022) · Cancers · doi:10.3390/cancers14194909 · Cited by 59 · see citing works
Determination of Antibacterial and Technological Properties of Vaginal Lactobacilli for Their Potential Application in Dairy Products
Siroli, L., Patrignani, F., Serrazanetti, D. I., Parolin, C., Palomino, R. A. Ñ., Vitali, B., & Lanciotti, R. (2017) · Frontiers in Microbiology · doi:10.3389/fmicb.2017.00166 · Cited by 59 · see citing works
The Role of Skin and Orogenital Microbiota in Protective Immunity and Chronic Immune-Mediated Inflammatory Disease
Park, Y. J., & Lee, H. K. (2018) · Frontiers in Immunology · doi:10.3389/fimmu.2017.01955 · Cited by 58 · see citing works
Parallel detection of lactobacillus and bacterial vaginosis-associated bacterial DNA in the chorioamnion and vagina of pregnant women at term
Lannon, S., Waldorf, K. M. A., Fiedler, T. L., Kapur, R. P., Agnew, K. J., Rajagopal, L., Gravett, M. G., & Fredricks, D. N. (2018) · The Journal of Maternal-Fetal & Neonatal Medicine · doi:10.1080/14767058.2018.1446208 · Cited by 55 · see citing works
Impact of Estrogen and Progesterone on Immune Cells and Host–Pathogen Interactions in the Lower Female Reproductive Tract
Collins, M. K., McCutcheon, C. R., & Petroff, M. G. (2022) · The Journal of Immunology · doi:10.4049/jimmunol.2200454 · Cited by 54 · see citing works
Lactobacilli biology, applications and host interactions
Mejía-Caballero, A., & Marco, M. L. (2025) · Nature Reviews Microbiology · doi:10.1038/s41579-025-01205-7 · Cited by 60 · see citing works
Gardnerella vaginalis diversity and ecology in relation to vaginal symptoms
Schellenberg, J. J., Patterson, M. H., & Hill, J. E. (2017) · Research in Microbiology · doi:10.1016/j.resmic.2017.02.011 · Cited by 54 · see citing works
The promising biological role of postbiotics derived from probiotic Lactobacillus species in reproductive health
Abbasi, A., Aghebati-Maleki, L., & Rad, A. H. (2021) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2021.1935701 · Cited by 53 · see citing works
Lactobacillus crispatus BC5 Interferes With Chlamydia trachomatis Infectivity Through Integrin Modulation in Cervical Cells
Parolin, C., Frisco, G., Foschi, C., Giordani, B., Salvo, M., Vitali, B., Marangoni, A., & Calonghi, N. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.02630 · Cited by 52 · see citing works
Endocervical and vaginal microbiota in South African adolescents with asymptomatic Chlamydia trachomatis infection
Balle, C., Lennard, K. S., Dabee, S., Barnabas, S. L., Jaumdally, S. Z., Gasper, M. A., Maseko, V., Mbulawa, Z. Z. A., Williamson, A., Bekker, L., et al. (2018) · Scientific Reports · doi:10.1038/s41598-018-29320-x · Cited by 52 · see citing works
Probiotic Lactobacillus sp. Strains Inhibit Growth, Adhesion, Biofilm Formation, and Gene Expression of Bacterial Vaginosis-Inducing Gardnerella vaginalis
Qian, Z., Zhu, H., Zhao, D., Yang, P., Gao, F., Lu, C., Yin, Y., Kan, S., & Chen, D. (2021) · Microorganisms · doi:10.3390/microorganisms9040728 · Cited by 51 · see citing works
Interaction of Gardnerella vaginalis and Vaginolysin with the Apical versus Basolateral Face of a Three-Dimensional Model of Vaginal Epithelium
Garcia, E. M., Kraskauskiene, V., Koblinski, J. E., & Jefferson, K. K. (2019) · Infection and Immunity · doi:10.1128/iai.00646-18 · Cited by 50 · see citing works
Complex species and strain ecology of the vaginal microbiome from pregnancy to postpartum and association with preterm birth
Pace, R. M., Chu, D. M., Prince, A., Ma, J., Seferovic, M. D., & Aagaard, K. M. (2021) · Med · doi:10.1016/j.medj.2021.06.001 · Cited by 48 · see citing works
In VitroActivity of Lactobacilli with Probiotic Potential Isolated from Cocoa Fermentation againstGardnerella vaginalis
Pessoa, W. F. B., Melgaço, A. C. C., de Almeida, M. E., Ramos, L. P., Rezende, R. P., & Romano, C. C. (2017) · BioMed Research International · doi:10.1155/2017/3264194 · Cited by 46 · see citing works
Repurposing Fenamic Acid Drugs To Combat Multidrug-Resistant Neisseria gonorrhoeae
Seong, Y. J., Alhashimi, M., Mayhoub, A. S., Mohammad, H. T., & Seleem, M. N. (2020) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.02206-19 · Cited by 39 · see citing works
Saccharomyces cerevisiae–based probiotic as novel anti-microbial agent for therapy of bacterial vaginosis
Sabbatini, S., Monari, C., Ballet, N., Mosci, P., Decherf, A. C., Pélerin, F., Perito, S., Scarpelli, P. T., & Vecchiarelli, A. (2018) · Virulence · doi:10.1080/21505594.2018.1464362 · Cited by 37 · see citing works
Roles of vaginal flora in human papillomavirus infection, virus persistence and clearance
Mi, Z., Li, X., Jiao, X., Cai, X., Yao, F., Xu, S., Huang, X., Zhang, Q., & Chen, J. (2023) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2022.1036869 · Cited by 38 · see citing works
Lactobacillus rhamnosus and Lactobacillus casei Affect Various Stages of Gardnerella Species Biofilm Formation
He, Y., Na, R., Niu, X., Xiao, B., & Yang, H. (2021) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2021.568178 · Cited by 35 · see citing works
Establishing an invertebrate Galleria mellonella greater wax moth larval model of Neisseria gonorrhoeae infection
Dijokaite-Guraliuc, A., Humbert, M. V., Borkowski, E. A., Ragione, R. M. L., & Christodoulides, M. (2021) · Virulence · doi:10.1080/21505594.2021.1950269 · Cited by 34 · see citing works
Differential effects of depot medroxyprogesterone acetate administration on vaginal microbiome in Hispanic White and Black women
Yang, L., Hao, Y., Hu, J., Kelly, D., Li, H., Brown, S. M., Tasker, C., Roche, N., Chang, T. L.-Y., & Pei, Z. (2019) · Emerging Microbes & Infections · doi:10.1080/22221751.2018.1563458 · Cited by 32 · see citing works
Thymbra Capitata Essential Oil as Potential Therapeutic Agent Against Gardnerella Vaginalis Biofilm-Related Infections
Machado, D., Gaspar, C., Palmeira‐de‐Oliveira, A., Cavaleiro, C., Salgueiro, L., Martinez‐de‐Oliveira, J., & Cerca, N. (2017) · Future Microbiology · doi:10.2217/fmb-2016-0184 · Cited by 31 · see citing works
Living Therapeutics: The Next Frontier of Precision Medicine
Kelly, V. W., Liang, B. K., & Sirk, S. J. (2020) · ACS Synthetic Biology · doi:10.1021/acssynbio.0c00444 · Cited by 30 · see citing works
Deciphering the role of female reproductive tract microbiome in reproductive health: a review
Gao, H., Liu, Q., Wang, X., Li, T., Li, H., Li, G., Tan, L., & Chen, Y. (2024) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2024.1351540 · Cited by 34 · see citing works
Role of the human vaginal microbiota in the regulation of inflammation and sexually transmitted infection acquisition: Contribution of the non-human primate model to a better understanding?
Adapen, C., Réot, L., & Menu, E. (2022) · Frontiers in Reproductive Health · doi:10.3389/frph.2022.992176 · Cited by 29 · see citing works
Contribution of the swine model in the study of human sexually transmitted infections
Käser, T., Renois, F., Wilson, H. L., Cnudde, T., Gerdts, V., Dillon, J. R., Jungersen, G., Agerholm, J. S., & Meurens, F. (2017) · Infection Genetics and Evolution · doi:10.1016/j.meegid.2017.11.022 · Cited by 28 · see citing works
Cervicovaginal microbiota significantly changed for HPV-positive women with high-grade squamous intraepithelial lesion
Guo, C., Dai, W., Zhou, Q., Gui, L., Cai, H., Wu, D., Hou, J., Li, C., Li, S. C., Du, H., et al. (2022) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2022.973875 · Cited by 28 · see citing works
Cervicovaginal Microbiome Factors in Clearance of Human Papillomavirus Infection
Dai, W., Du, H., Li, S. C., & Wu, R. (2021) · Frontiers in Oncology · doi:10.3389/fonc.2021.722639 · Cited by 27 · see citing works
Characterization and in vitro evaluation of a vaginal gel containing Lactobacillus crispatus for the prevention of gonorrhea
Gnaman, K. C. N., Bouttier, S., Yeo, A., AKA-ANY-GRAH, S., Geiger, S., Huang, N., Nicolas, V., Villebrun, S., Faye-Ketté, H., Ponchel, G., et al. (2020) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2020.119733 · Cited by 26 · see citing works
Interaction of vaginal Lactobacillus strains with HeLa cells plasma membrane
Calonghi, N., Parolin, C., Sartor, G., Verardi, L., Giordani, B., Frisco, G., Marangoni, A., & Vitali, B. (2017) · Beneficial Microbes · doi:10.3920/bm2016.0212 · Cited by 26 · see citing works
Comparative metagenomic analysis of the vaginal microbiome in healthy women
Liu, F., Zhou, Y., Zhu, L., Wang, Z., Ma, L., He, Y., & Fu, P. (2021) · Synthetic and Systems Biotechnology · doi:10.1016/j.synbio.2021.04.002 · Cited by 23 · see citing works
Lactiplantibacillus plantarum strains isolated from spontaneously fermented cocoa exhibit potential probiotic properties against Gardnerella vaginalis and Neisseria gonorrhoeae
Selis, N. D. N., Oliveira, H. B. M., Leão, H. F., dos Anjos, Y. B., Sampaio, B. A., Correia, T. M. L., Almeida, C. F., Pena, L. S. C., Reis, M. M., Brito, T. L. S., et al. (2021) · BMC Microbiology · doi:10.1186/s12866-021-02264-5 · Cited by 23 · see citing works
Pathogenic Interplay Between Chlamydia trachomatis and Neisseria gonorrhoeae that Influences Management and Control Efforts—More Questions than Answers?
Leonard, C. A., Schoborg, R. V., Low, N. N., Unemo, M., & Borel, N. (2019) · Current Clinical Microbiology Reports · doi:10.1007/s40588-019-00125-4 · Cited by 24 · see citing works
The role of vaginal microecology in the cervical cancer
Wei, W., Xie, L., Xia, Q. H., Fu, Y., Liu, F., Ding, D., & Han, F. (2022) · Journal of obstetrics and gynaecology research · doi:10.1111/jog.15359 · Cited by 22 · see citing works
Impact of oestrus synchronization devices on ewes vaginal microbiota and artificial insemination outcome
Reinoso-Peláez, E. L., Saura, M., González-Recio, Ó., González, C., Fernández, A., Peiró‐Pastor, R., López-García, A., Saborío‐Montero, A., Calvo, J. H., Ramón, M., et al. (2023) · Frontiers in Microbiology · doi:10.3389/fmicb.2023.1063807 · Cited by 22 · see citing works
Sexually transmitted infections among women randomised to depot medroxyprogesterone acetate, a copper intrauterine device or a levonorgestrel implant
Deese, J., Philip, N. M., Lind, M. L., Ahmed, K., Batting, J. R., Beksinska, M. E., Edward, V., Louw, C. E., Onono, M. A., Palanee‐Phillips, T., et al. (2020) · Sexually Transmitted Infections · doi:10.1136/sextrans-2020-054590 · Cited by 20 · see citing works
Mini-review: from in vitro to ex vivo studies: an overview of alternative methods for the study of medical biofilms
Castelo‐Branco, D. D. S. C. M., Amando, B. R., Ocadaque, C. J., de Aguiar, L., Paiva, D. D. D. Q., Diógenes, E. M., Guedes, G. M. D. M., Costa, C. L., Santos-Filho, A. S. P., de Andrade, A. R. C., et al. (2020) · Biofouling · doi:10.1080/08927014.2020.1859499 · Cited by 21 · see citing works
Consideration of Cultural Practices When Characterizing the Vaginal Microbiota Among African and African American Women
Nsereko, E., Moreland, P., Dunlop, A. L., Nzayirambaho, M., & Corwin, E. J. (2020) · Biological Research For Nursing · doi:10.1177/1099800420940788 · Cited by 19 · see citing works
Review of the evidence of the effects of human papillomavirus infection and Gardnerella vaginalis, and their co-infection on infertility
Gholizadeh, O., Yekanipour, Z., Kivi, S., Farahbod, F., Aqayinejad, M., Tasoujizade, Y., Yasamineh, S., Amini, P., Akbarzadeh, S., Hajiesmaeili, M., et al. (2023) · Microbial Pathogenesis · doi:10.1016/j.micpath.2023.105980 · Cited by 18 · see citing works
Lactobacillus strains vary in their ability to interact with human endometrial stromal cells
Shiroda, M., & Manning, S. D. (2020) · PLoS ONE · doi:10.1371/journal.pone.0238993 · Cited by 17 · see citing works
Tissue Models for Neisseria gonorrhoeae Research—From 2D to 3D
Heydarian, M., Rühl, E., Rawal, R., & Kozjak‐Pavlovic, V. (2022) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2022.840122 · Cited by 15 · see citing works
Microbiota changes impact on sexually transmitted infections and the development of pelvic inflammatory disease
Loeper, N., Graspeuntner, S., & Rupp, J. (2018) · Microbes and Infection · doi:10.1016/j.micinf.2018.02.003 · Cited by 15 · see citing works
Cervical Microbiome in Women Infected with HPV16 and High-Risk HPVs
Sasivimolrattana, T., Chantratita, W., Sensorn, I., Chaiwongkot, A., Oranratanaphan, S., Bhattarakosol, P., & Bhattarakosol, P. (2022) · International Journal of Environmental Research and Public Health · doi:10.3390/ijerph192214716 · Cited by 14 · see citing works
Microdroplet co-cultivation and interaction characterization of human vaginal bacteria
Jackman, C. M., Deans, K. W., Forney, L. J., & Lin, X. N. (2019) · Integrative Biology · doi:10.1093/intbio/zyz006 · Cited by 14 · see citing works
Highlight signatures of vaginal microbiota and metabolome contributed to the occurrence and recurrence of vulvovaginal candidiasis
Liang, Y., Huang, Z., Fan, S., Li, C., Huang, L.-T., Huang, C., Hutchins, A. P., Fang, C., & Zhang, X. (2024) · Microbiology Spectrum · doi:10.1128/spectrum.01521-24 · Cited by 13 · see citing works
Lactation and menstruation shift the vaginal microbiota in captive rhesus monkeys to be more similar to the male urethral microbiota
Hallmaier–Wacker, L. K., Lüert, S., Roos, C., & Knauf, S. (2019) · Scientific Reports · doi:10.1038/s41598-019-53976-8 · Cited by 16 · see citing works
Metagenomic analysis reveals the novel role of vaginal Lactobacillus iners in Chinese healthy pregnant women
Wang, X., Jiang, Q., Tian, X., Chen, W., Mai, J., Lin, G., Huo, Y., Zheng, H., Yan, D., Wang, X., et al. (2025) · npj Biofilms and Microbiomes · doi:10.1038/s41522-025-00731-9 · Cited by 12 · see citing works
The Penis, the Vagina and HIV Risk: Key Differences (Aside from the Obvious)
Kaul, R., Liu, C. M., Park, D. E., Galiwango, R. M., Tobian, A. A. R., & Prodger, J. L. (2022) · Viruses · doi:10.3390/v14061164 · Cited by 14 · see citing works
Gardnerella vaginalis and Neisseria gonorrhoeae Are Effectively Inhibited by Lactobacilli with Probiotic Properties Isolated from Brazilian Cupuaçu (Theobroma grandiflorum) Fruit
Selis, N. D. N., Oliveira, H. B. M., dos Anjos, Y. B., Leão, H. F., Sampaio, B. A., Correia, T. M. L., Reis, M. M., Brito, T. L. S., Almeida, C. F., Pena, L. S. C., et al. (2021) · BioMed Research International · doi:10.1155/2021/6626249 · Cited by 10 · see citing works
Variation of the Vaginal Lactobacillus Microbiome in Cytolytic Vaginosis
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P06.09 Lactobacilluscrispatus inhibits growth ofgardnerella vaginalisandneisseria gonorrhoeaeon a porcine vaginal mucosa model
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Authors: Breshears, L. M., Edwards, V. L., Ravel, J., & Peterson, M. L.
Affiliations: University of Minnesota (US)
University of Maryland, Baltimore (US)
Corresponding author: Peterson, Marnie L.
ORCID: Breshears, Laura M.
Edwards, Vonetta L.
Ravel, Jacques
Peterson, Marnie L.
Venue: BMC Microbiology
Publisher: Springer Science and Business Media LLC
Published: 2015-12
Volume: 15
Issue: 1
Pages: 276-276
ISSN: 1471-2180 (ISSN-L)
DOI: 10.1186/s12866-015-0608-0
PMC: PMC4675025
OpenAlex: W2192796897
Cited by: 140
References: 82
Citations by year: 2026: 10 · 2025: 12 · 2024: 22 · 2023: 12 · 2022: 13 · 2021: 17 · 2020: 15 · 2019: 11 · 2018: 13 · 2017: 12 · 2016: 3
FWCI: 7.86
Type: journal article
Language: en
Open access: gold
doi.org
Licence: cc-by
creativecommons.org/licenses/by/4.0/ (tdm)
creativecommons.org/licenses/by/4.0/ (vor, 8 d)
Locations: BMC Microbiology (publishedVersion, cc-by)
Europe PMC (PubMed Central) (publishedVersion, cc-by)
PubMed Central (publishedVersion, cc-by)
PubMed (publishedVersion)
Funders: University of Minnesota (22928)
National Institute of Allergy and Infectious Diseases (U19AI084044)
Topics: Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
Keywords: Lactobacillus crispatus, Gardnerella vaginalis, Neisseria gonorrhoeae, bacterial vaginosis, vaginal microbiota, biofilm formation, lactic acid, vaginal pH, sexually transmitted infections, vaginal colonization
MeSH terms: Animals, Antibiosis, Carboxylic Acids, Female, Gardnerella vaginalis, Humans, Hydrogen-Ion Concentration, Lactobacillus, Male, Mucous Membrane, Neisseria gonorrhoeae, Organ Culture Techniques, Swine, Vagina, Models, Animal

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Lactobacillus crispatus inhibits growth of Gardnerella vaginalis and Neisseria gonorrhoeae on a porcine vaginal mucosa model

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