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Cited by 71Reproductive tract infections researchProbiotics and Fermented FoodsGut microbiota and health

Diverse Expression of Antimicrobial Activities Against Bacterial Vaginosis and Urinary Tract Infection Pathogens by Cervicovaginal Microbiota Strains of Lactobacillus gasseri and Lactobacillus crispatus

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Atassi, Fabrice, Pho Viet Ahn, Diane L., Lievin-Le Moal, Vanessa·2019·Frontiers in Microbiology
DOI 10.3389/fmicb.2019.02900PMC PMC6933176
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Abstract

Objectives: We aimed to analyze the strain-by-strain expression of a large panel of antimicrobial activities counteracting the virulence mechanisms of bacterial vaginosis-associated P. bivia and G. vaginalis, pyelonephritis-associated E. coli, and recurrent cystitis- and preterm labor-associated E. coli by L. gasseri and L. crispatus strains resident of the cervicovaginal microbiota of healthy women. Methods: We evaluated the antimicrobial properties of human cervicovaginal microbiota-associated L. gasseri and L. crispatus cells and secreted molecules, focusing on bactericidal and bacteriostatic effects on free, adhering, or internalized pathogens and biofilm disrupting activity; the inhibition of pathogen association with cervix epithelial cells by competition, exclusion, or displacement; and protective properties against the deleterious cellular effects of specific pathogen toxins. Results: We found that no antimicrobial activity is specific to resident L. gasseri or L. crispatus species. Non-strain specific antimicrobial activity was exerted by secreted lactic acid, which killed the microbial pathogens by direct contact. The Lactobacillus cells exhibited another non-strain specific activity which inhibited the association of microbial pathogens with cervix epithelial cells with varying efficiency, partially protecting the cells against lysis and detachment triggered by toxin-producing G. vaginalis and uropathogenic E. coli. Potent strain-specific bactericidal activity was exerted by a limited number of resident L. gasseri strains with the capacity to produce and secrete antibiotic-like compounds. These compounds eradicated the microbial pathogens pre-associated with the surface of cervix epithelial cells, providing efficient protection of the cells against the deleterious effects triggered by toxin-producing G. vaginalis and uropathogenic E. coli. Furthermore, these compounds crossed the cell membrane to kill the pre-internalized microbial pathogens. Conclusion: Our findings show that human microbiota-associated cervicovaginal strains of L. gasseri and L. crispatus share common lactic acid-associated antimicrobial activities. Our results also provide evidence of strain-level specificity for certain antimicrobial properties among cervicovaginal L. gasseri and L. crispatus strains, indicating that the presence of a particular species in the vaginal microbiota is not sufficient to determine its benefit to the host. A full repertory of strain-specific properties should be evaluated in choosing vaginal microbiota-associated Lactobacillus isolates for the development of live biotherapeutic strategies

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Authors
Atassi, F., Pho Viet Ahn, D. L., & Lievin-Le Moal, V.
Affiliations
Inserm (FR)
Venue
Frontiers in Microbiology
Publisher
Frontiers Media SA
Published
2019-12-20
Volume
10
Pages
2900-2900
ISSN
1664-302X (ISSN-L)
DOI
10.3389/fmicb.2019.02900
PMC
PMC6933176
OpenAlex
W2995296462
Cited by
71
References
3
Citations by year
2026: 8 · 2025: 7 · 2024: 8 · 2023: 18 · 2022: 11 · 2021: 11 · 2020: 8
FWCI
5.34
Type
journal article
Language
en
Open access
Licence
Locations
  • Frontiers in Microbiology (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Agence Nationale de la Recherche (10-LABX-33, ANR-10, ANR-10-LABX, ANR-10-LABX-0033, ANR‐10‐LABX‐33)
  • Centre National de la Recherche Scientifique (ANR-10-LABX-33, UMR 8076)
  • Laboratoire d'Excellence en Recherche sur le Médicament et l'Innovation Thérapeutique (ANR-10-LABX-33)
Topics
  • Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
Keywords
Lactobacillus gasseri, Lactobacillus crispatus, cervicovaginal microbiome, antimicrobial activity, bacterial vaginosis, urinary tract infection, Gardnerella vaginalis, uropathogenic Escherichia coli, lactic acid, bactericidal activity, bacteriostatic activity, biofilm eradication, pathogen internalization, vaginal microbiota

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