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Cited by 179Gut microbiota and healthCystic Fibrosis Research AdvancesGastrointestinal motility and disorders

The altered gut microbiota in adults with cystic fibrosis

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Burke, D.G., Fouhy, F., Harrison, M. J., Rea, M. C., Cotter, P. D., O’Sullivan, O., Stanton, C., Hill, C. +3 more·2017·BMC Microbiology
DOI 10.1186/s12866-017-0968-8PMC PMC5345154
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Abstract

Cystic Fibrosis (CF) is an autosomal recessive disease that affects the function of a number of organs, principally the lungs, but also the gastrointestinal tract. The manifestations of cystic fibrosis transmembrane conductance regulator (CFTR) dysfunction in the gastrointestinal tract, as well as frequent antibiotic exposure, undoubtedly disrupts the gut microbiota. To analyse the effects of CF and its management on the microbiome, we compared the gut microbiota of 43 individuals with CF during a period of stability, to that of 69 non-CF controls using 454-pyrosequencing of the 16S rRNA gene. The impact of clinical parameters, including antibiotic therapy, on the results was also assessed. The CF-associated microbiome had reduced microbial diversity, an increase in Firmicutes and a reduction in Bacteroidetes compared to the non-CF controls. While the greatest number of differences in taxonomic abundances of the intestinal microbiota was observed between individuals with CF and the healthy controls, gut microbiota differences were also reported between people with CF when grouped by clinical parameters including % predicted FEV1 (measure of lung dysfunction) and the number of intravenous (IV) antibiotic courses in the previous 12 months. Notably, CF individuals presenting with severe lung dysfunction (% predicted FEV1 ≤ 40%) had significantly (p < 0.05) reduced gut microbiota diversity relative to those presenting with mild or moderate dysfunction. A significant negative correlation (−0.383, Simpson’s Diversity Index) was also observed between the number of IV antibiotic courses and gut microbiota diversity. This is one of the largest single-centre studies on gut microbiota in stable adults with CF and demonstrates the significantly altered gut microbiota, including reduced microbial diversity seen in CF patients compared to healthy controls. The data show the impact that CF and it's management have on gut microbiota, presenting the opportunity to develop CF specific probiotics to minimise microbiota alterations.

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  • In cystic fibrosis, bacteria generally considered markers of a healthy gut microbial profile such as Faecalibacterium, Roseburia, Akkermansia, and Bifidobacterium were decreased in a single-centre study on 43 individuals.

    “In cystic fibrosis, bacteria generally considered markers of a healthy gut microbial profile such as Faecalibacterium, Roseburia, Akkermansia, and Bifidobacterium, were shown be decreased in a single-centre study on 43 individuals with cystic fibrosis [95].”

    From Effect of Diet on the Gut Microbiota: Rethinking Intervention Duration (2019) · cites this paper as [95]

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  1. [30]Madan JC, Koestler DC, Stanton BA, Davidson L, Moulton LA, Housman ML, Moore JH, Guill MF, Morrison HG, Sogin ML et al. Serial analysis of the gut and respiratory microbiome in cystic fibrosis in infancy: interaction between intestinal and respiratory tracts and impact of nutritional exposures. mBio. 2012;3(4):e00251-12. PMC3428694 22911969

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Authors
Burke, D. G., Fouhy, F., Harrison, M. J., Rea, M. C., Cotter, P. D., O’Sullivan, O., Stanton, C., Hill, C., Shanahan, F., Plant, B. J., & Ross, R. P.
Affiliations
  • Teagasc - The Irish Agriculture and Food Development Authority (IE)
  • University College Cork (IE)
  • National University of Ireland (IE)
Corresponding author
Ross, R. P.
ORCID
Venue
BMC Microbiology
Publisher
Springer Science and Business Media LLC
Published
2017-03-09
Volume
17
Issue
1
Pages
58-58
ISSN
1471-2180 (ISSN-L)
DOI
10.1186/s12866-017-0968-8
PMC
PMC5345154
OpenAlex
W2591617823
Cited by
179
References
58
Citations by year
2026: 10 · 2025: 19 · 2024: 39 · 2023: 18 · 2022: 29 · 2021: 11 · 2020: 22 · 2019: 12 · 2018: 16 · 2017: 2
FWCI
5.81
Type
journal article
Language
en
Update notices
correction (2017-04-27)
Open access
Licence
cc-by
Locations
  • BMC Microbiology (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Arrow@dit (Dublin Institute of Technology) (acceptedVersion)
  • Arrow@dit (Dublin Institute of Technology) (submittedVersion, cc-by)
  • Cork Open Research Archive (University College Cork, Ireland) (submittedVersion, cc-by)
  • T-Stór (Teagasc) (submittedVersion, cc-by-nc-sa)
Funders
  • Science Foundation of Ireland (02/CE/B124)
  • Seventh Framework Programme (603038)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Cystic Fibrosis Research Advances
  • Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
Keywords
cystic fibrosis, gut microbiota, microbiome diversity, CFTR dysfunction, antibiotic exposure, intravenous antibiotics, lung function, FEV1, Firmicutes, Bacteroidetes, probiotics, adults with cystic fibrosis
MeSH terms
Firmicutes, Bacillota, Gastrointestinal Microbiome, Adult, Aged, Aged, 80 and over, Anti-Bacterial Agents, Bacteria, Classification, Cystic Fibrosis, DNA, Bacterial, Feces, Female, Humans, Male, Middle Aged, Phenotype, RNA, Ribosomal, 16S, Species Specificity, Probiotics, Bacteroidetes, Gastrointestinal Tract, Biodiversity, Metagenome, Administration, Intravenous

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