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Dynamic changes of CX3CL1/CX3CR1 axis during microglial activation and motor neuron loss in the spinal cord of ALS mouse model

Zhang, Jingjing, Liu, Yufei, Liu, Xinyao, Li, Song, Cheng, Cheng, Chen, Sheng, Le, Weidong·2018·Translational Neurodegeneration
DOI 10.1186/s40035-018-0138-4PMC PMC6309063
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Fig. 1 CX3CL1 were mainly located in neurons and decreased during MNs loss in TG mice. a Double immunofluorescent staining of CX3CL1 (green) and NeuN (red) in the anterior horn of lumber spinal cord of WT and TG mice at the age of 40, 90 and 120 days. Scale bar = 50 μm. b Integrated density of CX3CL1 staining. The values are expressed as mean ± SD, *p < 0.05 versus age-matched WT mice, ** p < 0.001 versus age-matched WT mice, n = 5 in each group
Fig. 1 CX3CL1 were mainly located in neurons and decreased during MNs loss in TG mice. a Double immunofluorescent staining of CX3CL1 (green) and NeuN (red) in the anterior horn of lumber spinal cord of WT and TG mice at the age of 40, 90 and 120 days. Scale bar = 50 μm. b Integrated density of CX3CL1 staining. The values are expressed as mean ± SD, *p < 0.05 versus age-matched WT mice, ** p < 0.001 versus age-matched WT mice, n = 5 in each groupCC BY · PubMed Central
Fig. 2 The mRNA and protein levels of CX3CL1 in the spinal cord anterior horns. a qPCR analysis of CX3CL1 mRNA level. b Western blot analysis of CX3CL1 protein level. c Quantitative analysis the CX3CL1 Protein level. The values are expressed as mean ± SD, *p < 0.05 versus age-matched WT mice, ** p < 0.001 versus age-matched WT mice, n = 5 in each group
Fig. 2 The mRNA and protein levels of CX3CL1 in the spinal cord anterior horns. a qPCR analysis of CX3CL1 mRNA level. b Western blot analysis of CX3CL1 protein level. c Quantitative analysis the CX3CL1 Protein level. The values are expressed as mean ± SD, *p < 0.05 versus age-matched WT mice, ** p < 0.001 versus age-matched WT mice, n = 5 in each groupCC BY · PubMed Central
Fig. 3 CX3CR1 was mainly located in microglia and was increased in the TG mice at the age of 90 and 120 days. a Double immunofluorescent staining of CX3CR1 (green) and Iba1(red) in the anterior horn of lumber spinal cord of WT and TG mice at the age of 40, 90 and 120 days. Scale bar = 50 μm. b Number of CX3CR1+/Iba1+ microglia per mm2. The values are expressed as mean ± SD, *p < 0.05 versus age-matched WT mice, ** p < 0.001 versus age-matched WT mice, n = 5 in each group
Fig. 3 CX3CR1 was mainly located in microglia and was increased in the TG mice at the age of 90 and 120 days. a Double immunofluorescent staining of CX3CR1 (green) and Iba1(red) in the anterior horn of lumber spinal cord of WT and TG mice at the age of 40, 90 and 120 days. Scale bar = 50 μm. b Number of CX3CR1+/Iba1+ microglia per mm2. The values are expressed as mean ± SD, *p < 0.05 versus age-matched WT mice, ** p < 0.001 versus age-matched WT mice, n = 5 in each groupCC BY · PubMed Central
Fig. 4 The mRNA and protein levels of CX3CR1 in the spinal cord anterior horns. a qPCR analysis of CX3CR1 mRNA level. b Western blot analysis of CX3CR1 protein level. c Quantitative analysis the CX3CR1 Protein level. The values are expressed as mean ± SD, *p < 0.05 versus age-matched WT mice, ** p < 0.001 versus age-matched WT mice, n = 5 in each group
Fig. 4 The mRNA and protein levels of CX3CR1 in the spinal cord anterior horns. a qPCR analysis of CX3CR1 mRNA level. b Western blot analysis of CX3CR1 protein level. c Quantitative analysis the CX3CR1 Protein level. The values are expressed as mean ± SD, *p < 0.05 versus age-matched WT mice, ** p < 0.001 versus age-matched WT mice, n = 5 in each groupCC BY · PubMed Central
Fig. 5 The changes of M1 microglia in the TG mice at different time points. a Double immunofluorescent staining of CD86 (red) and Iba1 (green) at different time points, Sale bar = 50 μm. b Quantitative analysis of the M1 microglia per mm2 at different time points. The values are expressed as mean ± SD, *p < 0.05 versus age-matched WT mice, ** p < 0.001 versus age-matched WT mice, n = 5 in each group
Fig. 5 The changes of M1 microglia in the TG mice at different time points. a Double immunofluorescent staining of CD86 (red) and Iba1 (green) at different time points, Sale bar = 50 μm. b Quantitative analysis of the M1 microglia per mm2 at different time points. The values are expressed as mean ± SD, *p < 0.05 versus age-matched WT mice, ** p < 0.001 versus age-matched WT mice, n = 5 in each groupCC BY · PubMed Central
Fig. 6 The changes of M2 microglial phenotypes in TG mice at different time points. a Double immunofluorescent staining of Arg1 (green) and Iba1 (red) at different time points, Sale bar = 50 μm. b Quantitative analysis of the M2 microglia per mm2 at different time points. The values are expressed as mean ± SD, *p < 0.05 versus age-matched WT mice, ** p < 0.001 versus age-matched WT mice, n = 5 in each group
Fig. 6 The changes of M2 microglial phenotypes in TG mice at different time points. a Double immunofluorescent staining of Arg1 (green) and Iba1 (red) at different time points, Sale bar = 50 μm. b Quantitative analysis of the M2 microglia per mm2 at different time points. The values are expressed as mean ± SD, *p < 0.05 versus age-matched WT mice, ** p < 0.001 versus age-matched WT mice, n = 5 in each groupCC BY · PubMed Central
Fig. 7 The mRNA levels of M1/M2 microglial markers in the TG mice at different time points. a, b The mRNA levels of M1 markers (INOS, CD86). c, d The mRNA levels of M2 markers (Arg1, CD206). The values are expressed as mean ± SD, *p < 0.05 versus age-matched WT mice, ** p < 0.001 versus age-matched WT mice, n = 5 in each group
Fig. 7 The mRNA levels of M1/M2 microglial markers in the TG mice at different time points. a, b The mRNA levels of M1 markers (INOS, CD86). c, d The mRNA levels of M2 markers (Arg1, CD206). The values are expressed as mean ± SD, *p < 0.05 versus age-matched WT mice, ** p < 0.001 versus age-matched WT mice, n = 5 in each groupCC BY · PubMed Central
Fig. 8 The changes of M1/M2 microglia related cytokines mRNA levels at different time points. a, b mRNA levels of two cytokines related to M1 microglia (IL-1β and TNF-α). c mRNA levels of IL-10 related to M2 microglia. The values are expressed as mean ± SD, *p < 0.05 versus age-matched WT mice, ** p < 0.001 versus age-matched WT mice, n = 5 in each group
Fig. 8 The changes of M1/M2 microglia related cytokines mRNA levels at different time points. a, b mRNA levels of two cytokines related to M1 microglia (IL-1β and TNF-α). c mRNA levels of IL-10 related to M2 microglia. The values are expressed as mean ± SD, *p < 0.05 versus age-matched WT mice, ** p < 0.001 versus age-matched WT mice, n = 5 in each groupCC BY · PubMed Central

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Research works printed but not linked: 34

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Metadata

Authors
Zhang, J., Liu, Y., Liu, X., Li, S., Cheng, C., Chen, S., & Le, W.
Venue
Translational Neurodegeneration
Publisher
Springer Science and Business Media LLC
Published
2018-12
Volume
7
Issue
1
ISSN
2047-9158
DOI
10.1186/s40035-018-0138-4
PMC
PMC6309063
Cited by
57
References
6
Language
en
Licence
creativecommons.org/licenses/by/4.0/ (tdm)
Funders
  • the National Natural Sciences Foundation of China (NSFC 81430021, NSFC 81370470)
  • he Program for Liaoning Innovative Research Team in University (LT2015009)
  • Liaoning Science and Technology Project (2015225008)

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