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Multi-cohort analysis of colorectal cancer metagenome identified altered bacteria across populations and universal bacterial markers
Open accessAbstract
BACKGROUND: Alterations of gut microbiota are associated with colorectal cancer (CRC) in different populations and several bacterial species were found to contribute to the tumorigenesis. The potential use of gut microbes as markers for early diagnosis has also been reported. However, cohort specific noises may distort the structure of microbial dysbiosis in CRC and lead to inconsistent results among studies. In this regard, our study targeted at exploring changes in gut microbiota that are universal across populations at species level. RESULTS: Based on the combined analysis of 526 metagenomic samples from Chinese, Austrian, American, and German and French cohorts, seven CRC-enriched bacteria (Bacteroides fragilis, Fusobacterium nucleatum, Porphyromonas asaccharolytica, Parvimonas micra, Prevotella intermedia, Alistipes finegoldii, and Thermanaerovibrio acidaminovorans) have been identified across populations. The seven enriched bacterial markers classified cases from controls with an area under the receiver-operating characteristics curve (AUC) of 0.80 across the different populations. Abundance correlation analysis demonstrated that CRC-enriched and CRC-depleted bacteria respectively formed their own mutualistic networks, in which the latter was disjointed in CRC. The CRC-enriched bacteria have been found to be correlated with lipopolysaccharide and energy biosynthetic pathways. CONCLUSIONS: Our study identified potential diagnostic bacterial markers that are robust across populations, indicating their potential universal use for non-invasive CRC diagnosis. We also elucidated the ecological networks and functional capacities of CRC-associated microbiota.
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“A meta-analysis of 4 case–control studies of metagenomes of patients with CRC found that Bacteroides fragilis was the only species that was consistently enriched in intestinal microbiomes of patients with CRC worldwide.46”
From Influence of the Gut Microbiome, Diet, and Environment on Risk of Colorectal Cancer (2020) · cites this paper as [46]
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Metadata
- Authors
- Dai, Z., Coker, O. O., Nakatsu, G., Wu, W. K. K., Zhao, L., Chen, Z., Chan, F. K. L., Kristiansen, K., Sung, J. J. Y., Wong, S. H., & Yu, J.
- Affiliations
- Chinese University of Hong Kong (HK)
- BGI Group (China) (CN)
- Corresponding author
- Wong, Sunny Hei
- Yu, Jun
- ORCID
- Venue
- Microbiome
- Published
- 2018-04-11
- Volume
- 6
- Issue
- 1
- Pages
- 70-70
- ISSN
- 2049-2618 (ISSN-L)
- PMC
- PMC5896039
- OpenAlex
- W2796803893
- Cited by
- 545
- References
- 54
- Citations by year
- 2026: 43 · 2025: 70 · 2024: 99 · 2023: 97 · 2022: 67 · 2021: 78 · 2020: 53 · 2019: 29 · 2018: 8
- FWCI
- 14.94
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Microbiome (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, other-oa)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Research at the University of Copenhagen (University of Copenhagen) (publishedVersion, other-oa)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- RGC-GRF (766613, 14106145, 14111216)
- 135 Program Project China (2016YFC1303200)
- 973 Program China (2013CB531401)
- National Key Technology R&D Program (2014BAI09B05)
- Shenzhen Virtual University Park Support Scheme to CUHK Shenzhen Research Institute
- Croucher Foundation
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Health Sciences › Medicine › Infectious Diseases › Clostridium difficile and Clostridium perfringens research
- Keywords
- colorectal cancer, gut microbiota, metagenomics, bacterial markers, Bacteroides fragilis, Fusobacterium nucleatum, Porphyromonas asaccharolytica, Parvimonas micra, Prevotella intermedia, Alistipes finegoldii, dysbiosis, microbial co-occurrence networks, non-invasive cancer diagnosis
- MeSH terms
- Gastrointestinal Microbiome, Aged, Bacteria, Cell Transformation, Neoplastic, Female, Humans, Male, Middle Aged, Colorectal Neoplasms, Computational Biology, Metagenome, Metagenomics, Gene Ontology
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