Cited by 85Probiotics and Fermented FoodsGut microbiota and healthFermentation and Sensory Analysis

Metabolic framework of spontaneous and synthetic sourdough metacommunities to reveal microbial players responsible for resilience and performance

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Calabrese, Francesco Maria, Ameur, Hana, Nikoloudaki, Olga, Celano, Giuseppe, Vacca, Mirco, Junior, Wilson JFLemos, Manzari, Caterina, Vertè, Fabienne +4 more·2022·Microbiome

Abstract

Background: In nature, microbial communities undergo changes in composition that threaten their resiliency. Here, we interrogated sourdough, a natural cereal-fermenting metacommunity, as a dynamic ecosystem in which players are subjected to continuous environmental and spatiotemporal stimuli. Results: The inspection of spontaneous sourdough metagenomes and transcriptomes revealed dominant, subdominant and satellite players that are engaged in different functional pathways. The highest microbial richness was associated with the highest number of gene copies per pathway. Based on meta-omics data collected from 8 spontaneous sourdoughs and their identified microbiota, we de novo reconstructed a synthetic microbial community SDG. We also reconstructed SMC-SD43 from scratch using the microbial composition of its spontaneous sourdough equivalent for comparison. The KEGG number of dominant players in the SDG was not affected by depletion of a single player, whereas the subdominant and satellite species fluctuated, revealing unique contributions. Compared to SMC-SD43, SDG exhibited broader transcriptome redundancy. The invariant volatilome profile of SDG after in situ long-term back slopping revealed its stability. In contrast, SMC-SD43 lost many taxon members. Dominant, subdominant and satellite players together ensured gene and transcript redundancy. Conclusions: Our study demonstrates how, by starting from spontaneous sourdoughs and reconstructing these communities synthetically, it was possible to unravel the metabolic contributions of individual players. For resilience and good performance, the sourdough metacommunity must include dominant, subdominant and satellite players, which together ensure gene and transcript redundancy. Overall, our study changes the paradigm and introduces theoretical foundations for directing food fermentations.

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  • For resilience and optimal performance, the sourdough metacommunity includes dominant, subdominant, and satellite players, which together ensure gene and transcript redundancy.

    “For resilience and optimal performance, the sourdough metacommunity includes dominant, subdominant, and satellite players, which together ensure gene and transcript redundancy [122].”
  • In sourdough microbiota, microbial communities undergo compositional changes that challenge their resilience.

    “In sourdough microbiota, it has been observed that microbial communities undergo compositional changes that challenge their resilience.”

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  1. [62]De Vuyst L Van Kerrebroeck S Leroy F Microbial Ecology and Process Technology of Sourdough Fermentation Adv Appl Microbiol 2017 100 49 160 10.1016/bs.aambs.2017.02.003 28732554 · DOI · Paper page

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Authors
Calabrese, F. M., Ameur, H., Nikoloudaki, O., Celano, G., Vacca, M., Junior, W. J., Manzari, C., Vertè, F., Di Cagno, R., Pesole, G., De Angelis, M., & Gobbetti, M.
Affiliations
  • University of Bari Aldo Moro (IT)
  • Free University of Bozen-Bolzano (IT)
Venue
Microbiome
Published
2022-09-14
Volume
10
Issue
1
Pages
148-148
ISSN
2049-2618 (ISSN-L)
OpenAlex
W4295577481
Cited by
85
References
64
Citations by year
2026: 27 · 2025: 18 · 2024: 26 · 2023: 14
FWCI
7.71
Type
journal article
Language
en
Open access
Locations
  • Microbiome (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • CINECA IRIS Institutional Research Information System (University of Bari Aldo Moro) (submittedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Open Research (University of Surrey) (submittedVersion, cc-by)
Funders
Libera Università di Bolzano
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Fermentation and Sensory Analysis
Keywords
sourdough, synthetic microbial communities, metagenomics, metatranscriptomics, metaproteomics, community resilience, cereal fermentation, volatilome, backslopping, KEGG pathways
MeSH terms
Bread, Edible Grain, Fermentation, Food Microbiology, Microbiota

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