The DOI matches its Crossref record; 45 of the 45 references it cites were found in a registry or an index.
A simplified method for the preparation of detergent-free lipid rafts
Open accessAbstract
Lipid rafts are small plasma membrane domains that contain high levels of cholesterol and sphingolipids. Traditional methods for the biochemical isolation of lipid rafts involve the extraction of cells with nonionic detergents followed by the separation of a low-density, detergent-resistant membrane fraction on density gradients. Because of concerns regarding the possible introduction of artifacts through the use of detergents, it is important to develop procedures for the isolation of lipid rafts that do not involve detergent extraction. We report here a simplified method for the purification of detergent-free lipid rafts that requires only one short density gradient centrifugation, but yields a membrane fraction that is highly enriched in cholesterol and protein markers of lipid rafts, with no contamination from nonraft plasma membrane or intracellular membranes.
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Cites
A simplification of the protein assay method of Lowry et al. which is more generally applicable
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Lipid rafts and signal transduction
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Structure and Function of Sphingolipid- and Cholesterol-rich Membrane Rafts
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Partitioning of Lipid-Modified Monomeric GFPs into Membrane Microdomains of Live Cells
Zacharias, D. A., Violin, J. D., Newton, A. C., & Tsien, R. Y. (2002) · Science · doi:10.1126/science.1068539 · Cited by 2,235
The State of Lipid Rafts: From Model Membranes to Cells
Edidin, M. (2003) · Annual Review of Biophysics and Biomolecular Structure · doi:10.1146/annurev.biophys.32.110601.142439 · Cited by 1,341
Lipid rafts: bringing order to chaos
Pike, L. J. (2003) · Journal of Lipid Research · doi:10.1194/jlr.r200021-jlr200 · Cited by 1,138
Self-citationA Role for Lipid Shells in Targeting Proteins to Caveolae, Rafts, and Other Lipid Domains
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Caveolins, Liquid-Ordered Domains, and Signal Transduction
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Bersuker, K., Hendricks, J. M., Li, Z., Magtanong, L., Ford, B., Tang, P. H., Roberts, M. A., Tong, B., Maimone, T. J., Zoncu, R., et al. (2019) · Nature · doi:10.1038/s41586-019-1705-2 · Cited by 3,703
Upregulated function of mitochondria‐associated ER membranes in Alzheimer disease
Area‐Gomez, E., del Carmen Lara Castillo, M., Tambini, M. D., Guardia‐Laguarta, C., de Groof, A. J. C., Madra, M., Ikenouchi, J., Umeda, M., Bird, T. D., Sturley, S. L., et al. (2012) · The EMBO Journal · doi:10.1038/emboj.2012.202 · Cited by 638
Interaction of membrane/lipid rafts with the cytoskeleton: Impact on signaling and function
Head, B. P., Patel, H. H., & Insel, P. A. (2014) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/j.bbamem.2013.07.018 · Cited by 511
Zhu, X., Owen, J. S., Wilson, M. D., Li, H., Griffiths, G. L., Thomas, M. J., Hiltbold, E. M., Fessler, M. B., & Parks, J. S. (2010) · Journal of Lipid Research · doi:10.1194/jlr.m006486 · Cited by 331
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Metadata
- Authors
- Macdonald, J. L., & Pike, L. J.
- Venue
- Journal of Lipid Research
- Publisher
- Elsevier BV
- Published
- 2005-05
- Volume
- 46
- Issue
- 5
- Pages
- 1061-1067
- ISSN
- 0022-2275 (ISSN-L)
- OpenAlex
- W2157483796
- Cited by
- 339
- References
- 45
- FWCI
- 8.99
- Type
- journal article
- Language
- en
- Open access
- hybrid
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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