Cited by 228Metabolomics and Mass Spectrometry StudiesSphingolipid Metabolism and SignalingMass Spectrometry Techniques and Applications

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Comprehensive quantification of ceramide species in human stratum corneum

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Masukawa, Yoshinori, Narita, Hirofumi, Sato, Hirayuki, Naoe, Ayano, Kondo, Naoki, Sugai, Yoshiya, Oba, Tsuyoshi, Homma, Rika, Ishikawa, Junko, Takagi, Yutaka, Kitahara, Takashi·2009·Journal of Lipid Research

DOI 10.1194/jlr.d800055-jlr200PMC PMC2724059

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Abstract

One of the key challenges in lipidomics is to quantify lipidomes of interest, as it is practically impossible to collect all authentic materials covering the targeted lipidomes. For diverse ceramides (CER) in human stratum corneum (SC) that play important physicochemical roles in the skin, we developed a novel method for quantification of the overall CER species by improving our previously reported profiling technique using normal-phase liquid chromatography-electrospray ionization-mass spectrometry (NPLC-ESI-MS). The use of simultaneous selected ion monitoring measurement of as many as 182 kinds of molecular-related ions enables the highly sensitive detection of the overall CER species, as they can be analyzed in only one SC-stripped tape as small as 5 mm x 10 mm. To comprehensively quantify CERs, including those not available as authentic species, we designed a procedure to estimate their levels using relative responses of representative authentic species covering the species targeted, considering the systematic error based on intra-/inter-day analyses. The CER levels obtained by this method were comparable to those determined by conventional thin-layer chromatography (TLC), which guarantees the validity of this method. This method opens lipidomics approaches for CERs in the SC.

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Authors: Masukawa, Y., Narita, H., Sato, H., Naoe, A., Kondo, N., Sugai, Y., Oba, T., Homma, R., Ishikawa, J., Takagi, Y., & Kitahara, T.
Affiliations: Kao Corporation (Japan) (JP)
Corresponding author: Masukawa, Yoshinori
ORCID: Naoe, Ayano
Kondo, Naoki
Homma, Rika
Ishikawa, Junko
Takagi, Yutaka
Venue: Journal of Lipid Research
Publisher: Elsevier BV
Published: 2009-08
Volume: 50
Issue: 8
Pages: 1708-1719
ISSN: 0022-2275 (ISSN-L)
DOI: 10.1194/jlr.d800055-jlr200
PMC: PMC2724059
OpenAlex: W2143857102
Cited by: 228
References: 48
Citations by year: 2026: 5 · 2025: 7 · 2024: 9 · 2023: 10 · 2022: 16 · 2021: 15 · 2020: 10 · 2019: 12 · 2018: 20 · 2017: 18 · 2016: 18 · 2015: 11 · 2014: 11 · 2013: 19 · 2012: 30
FWCI: 6.18
Type: journal article
Language: en
Open access: hybrid
doi.org
Licence: cc-by
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
creativecommons.org/licenses/by/4.0/ (vor, 4039 d)
Locations: Journal of Lipid Research (publishedVersion, cc-by)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Sphingolipid Metabolism and Signaling
Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
Keywords: ceramide, stratum corneum, skin lipids, lipid quantification, normal-phase chromatography, electrospray ionization mass spectrometry, selected ion monitoring, skin barrier, lipidomics
MeSH terms: Adult, Cell Extracts, Ceramides, Cheek, Chromatography, Liquid, Epidermis, Fatty Acids, Forearm, Humans, Male, Spectrometry, Mass, Electrospray Ionization

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Comprehensive quantification of ceramide species in human stratum corneum

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