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Characterization of overall ceramide species in human stratum corneum
Open accessAbstract
Ceramides (CERs) in human stratum corneum (SC) play physicochemical roles in determining barrier and water-holding functions of the skin, and specific species might be closely related to the regulation of keratinization, together with other CER-related lipids. Structures of those diverse CER species, however, have not been comprehensively revealed. The aim of this study was to characterize overall CER species in the SC. First, we constructed 3D multi-mass chromatograms of the overall CER species, based on normal-phase liquid chromatography (NPLC) connected to electrospray ionization-mass spectrometry (ESI-MS) using a gradient elution system and a postcolumn addition of a volatile salt-containing polar solvent. The CERs targeted from the 3D chromatograms were structurally analyzed using NPLC-ESI-tandem mass spectrometry (MS/MS), which resulted in the identification of 342 CER species in the inner forearm SC. This led to the discovery of a new CER class consisting of alpha-hydroxy fatty acid and dihydrosphingosine moieties, in addition to the 10 classes generally known. The results also revealed that those CERs contain long-chain (more than C(18))-containing sphingoids and a great number of isobaric species. These novel results will contribute not only to physiochemical research on CERs in the SC but also to lipidomics approaches to CERs in the skin.
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“In the human SC, 16 ceramide classes other than SD and -hydroxy ceramides have been identified (5)(6)(7).”
From Comparative profiling and comprehensive quantification of stratum corneum ceramides in humans and mice by LC/MS/MS (2020) · cites this paper as [5]
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“Additionally, the fragment ion pattern of ceramides differs depending on their LCB moiety (5).”
From Comparative profiling and comprehensive quantification of stratum corneum ceramides in humans and mice by LC/MS/MS (2020) · cites this paper as [5]
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“Therefore, more than several hundred ceramide species exist in the human SC (5)(6)(7).”
From Comparative profiling and comprehensive quantification of stratum corneum ceramides in humans and mice by LC/MS/MS (2020) · cites this paper as [5]
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“The composition of ceramide classes/species in the human SC has previously been analyzed using LC/MS (5-7, 16, 17, 29, 30).”
From Comparative profiling and comprehensive quantification of stratum corneum ceramides in humans and mice by LC/MS/MS (2020) · cites this paper as [5]
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Metadata
- Authors
- Masukawa, Y., Narita, H., Shimizu, E., Kondo, N., Sugai, Y., Oba, T., Homma, R., Ishikawa, J., Takagi, Y., Kitahara, T., Takema, Y., & Kita, K.
- Affiliations
- Kao Corporation (Japan) (JP)
- Corresponding author
- Masukawa, Yoshinori
- Venue
- Journal of Lipid Research
- Publisher
- Elsevier BV
- Published
- 2008-07
- Volume
- 49
- Issue
- 7
- Pages
- 1466-1476
- ISSN
- 0022-2275 (ISSN-L)
- OpenAlex
- W2159423422
- Cited by
- 322
- References
- 52
- Citations by year
- 2026: 7 · 2025: 6 · 2024: 17 · 2023: 13 · 2022: 17 · 2021: 19 · 2020: 22 · 2019: 18 · 2018: 24 · 2017: 25 · 2016: 24 · 2015: 19 · 2014: 25 · 2013: 30 · 2012: 19
- FWCI
- 4.94
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Lipid Research (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Kao Corporation
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Sphingolipid Metabolism and Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advancements in Transdermal Drug Delivery
- Keywords
- ceramide, stratum corneum, skin barrier, dihydrosphingosine, keratinization, normal-phase chromatography, tandem mass spectrometry
- MeSH terms
- Ceramides, Humans, Skin, Mass Spectrometry, Molecular Structure
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