Comprehensive quantification of ceramide species in human stratum corneum
Open accessAbstract
One of the key challenges in lipidomics is to quantify lipidomes of interest, as it is practically impossible to collect all authentic materials covering the targeted lipidomes. For diverse ceramides (CER) in human stratum corneum (SC) that play important physicochemical roles in the skin, we developed a novel method for quantification of the overall CER species by improving our previously reported profiling technique using normal-phase liquid chromatography-electrospray ionization-mass spectrometry (NPLC-ESI-MS). The use of simultaneous selected ion monitoring measurement of as many as 182 kinds of molecular-related ions enables the highly sensitive detection of the overall CER species, as they can be analyzed in only one SC-stripped tape as small as 5 mm x 10 mm. To comprehensively quantify CERs, including those not available as authentic species, we designed a procedure to estimate their levels using relative responses of representative authentic species covering the species targeted, considering the systematic error based on intra-/inter-day analyses. The CER levels obtained by this method were comparable to those determined by conventional thin-layer chromatography (TLC), which guarantees the validity of this method. This method opens lipidomics approaches for CERs in the SC.
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Earlier studies have reported that ceramides with odd-chain FAs exist at high levels in the SC.
“Earlier studies have reported that ceramides with oddchain FAs exist at high levels in the SC (Fig. 3F, supplemental Table S3) (7,55).”
From Comparative profiling and comprehensive quantification of stratum corneum ceramides in humans and mice by LC/MS/MS (2020) · cites this paper as [55]
The Methods passage lists an odd-chain ceramide, CER[N(17)S(18)], as an internal standard, not as a ceramide found at high levels in stratum corneum. Searches for odd-chain fatty acids and their abundance found no passage in our copy reporting the claimed high levels; the paper discusses quantifying ceramide species generally. (LLM, conf=0.72)
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- Authors
- Masukawa, Y., Narita, H., Sato, H., Naoe, A., Kondo, N., Sugai, Y., Oba, T., Homma, R., Ishikawa, J., Takagi, Y., & Kitahara, T.
- Affiliations
- Kao Corporation (Japan) (JP)
- Corresponding author
- Masukawa, Yoshinori
- Venue
- Journal of Lipid Research
- Publisher
- Elsevier BV
- Published
- 2009-08
- Volume
- 50
- Issue
- 8
- Pages
- 1708-1719
- ISSN
- 0022-2275 (ISSN-L)
- PMC
- PMC2724059
- OpenAlex
- W2143857102
- Cited by
- 228
- References
- 48
- Citations by year
- 2026: 5 · 2025: 7 · 2024: 9 · 2023: 10 · 2022: 16 · 2021: 15 · 2020: 10 · 2019: 12 · 2018: 20 · 2017: 18 · 2016: 18 · 2015: 11 · 2014: 11 · 2013: 19 · 2012: 30
- FWCI
- 6.18
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Lipid Research (publishedVersion, cc-by)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Sphingolipid Metabolism and Signaling
- Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
- Keywords
- ceramide, stratum corneum, skin lipids, lipid quantification, normal-phase chromatography, electrospray ionization mass spectrometry, selected ion monitoring, skin barrier, lipidomics
- MeSH terms
- Adult, Cell Extracts, Ceramides, Cheek, Chromatography, Liquid, Epidermis, Fatty Acids, Forearm, Humans, Male, Spectrometry, Mass, Electrospray Ionization
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