The DOI matches its Crossref record; 52 of the 52 references it cites were found in a registry or an index.
Characterization of overall ceramide species in human stratum corneum
Open accessAbstract
Ceramides (CERs) in human stratum corneum (SC) play physicochemical roles in determining barrier and water-holding functions of the skin, and specific species might be closely related to the regulation of keratinization, together with other CER-related lipids. Structures of those diverse CER species, however, have not been comprehensively revealed. The aim of this study was to characterize overall CER species in the SC. First, we constructed 3D multi-mass chromatograms of the overall CER species, based on normal-phase liquid chromatography (NPLC) connected to electrospray ionization-mass spectrometry (ESI-MS) using a gradient elution system and a postcolumn addition of a volatile salt-containing polar solvent. The CERs targeted from the 3D chromatograms were structurally analyzed using NPLC-ESI-tandem mass spectrometry (MS/MS), which resulted in the identification of 342 CER species in the inner forearm SC. This led to the discovery of a new CER class consisting of alpha-hydroxy fatty acid and dihydrosphingosine moieties, in addition to the 10 classes generally known. The results also revealed that those CERs contain long-chain (more than C(18))-containing sphingoids and a great number of isobaric species. These novel results will contribute not only to physiochemical research on CERs in the SC but also to lipidomics approaches to CERs in the skin.
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“In the human SC, 16 ceramide classes other than SD and -hydroxy ceramides have been identified (5)(6)(7).”
From Comparative profiling and comprehensive quantification of stratum corneum ceramides in humans and mice by LC/MS/MS (2020) · cites this paper as [5]
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“Additionally, the fragment ion pattern of ceramides differs depending on their LCB moiety (5).”
From Comparative profiling and comprehensive quantification of stratum corneum ceramides in humans and mice by LC/MS/MS (2020) · cites this paper as [5]
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“Therefore, more than several hundred ceramide species exist in the human SC (5)(6)(7).”
From Comparative profiling and comprehensive quantification of stratum corneum ceramides in humans and mice by LC/MS/MS (2020) · cites this paper as [5]
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“The composition of ceramide classes/species in the human SC has previously been analyzed using LC/MS (5-7, 16, 17, 29, 30).”
From Comparative profiling and comprehensive quantification of stratum corneum ceramides in humans and mice by LC/MS/MS (2020) · cites this paper as [5]
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Citations
Cites
Functions of Ceramide in Coordinating Cellular Responses to Stress
Hannun, Y. A. (1996) · Science · doi:10.1126/science.274.5294.1855 · Cited by 1,612
Biochemistry of Lipids, Lipoproteins and Membranes
(2021) · Elsevier eBooks · doi:10.1016/c2020-0-00089-7 · Cited by 1,402
Epidermal Lipids, Barrier Function, and Desquamation
Elias, P. M. (1983) · Journal of Investigative Dermatology · doi:10.1038/jid.1983.12 · Cited by 1,356
Imokawa, G., Abe, A., Jin, K., Higaki, Y., Kawashima, M., & Hidano, A. (1991) · Journal of Investigative Dermatology · doi:10.1111/1523-1747.ep12470233 · Cited by 1,040
Ceramide in apoptosis: an overview and current perspectives
Pettus, B. J., Chalfant, C. E., & Hannun, Y. A. (2002) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/s1388-1981(02)00331-1 · Cited by 788
Nicolaides, N. (1974) · Science · doi:10.1126/science.186.4158.19 · Cited by 609
Zheng, W., Kollmeyer, J., Symolon, H., Momin, A. A., Munter, E., Wang, E. L., Kelly, S. L., Allegood, J. C., Liu, Y., Peng, Q., et al. (2006) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/j.bbamem.2006.08.009 · Cited by 561
Ceramide composition of the psoriatic scale
Motta, S., Monti, M., Sesana, S., Caputo, R., Carelli, S., & Ghidoni, R. (1993) · Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease · doi:10.1016/0925-4439(93)90135-n · Cited by 435
Siegenthaler, U., Laine, A. M., & Polák, L. (1983) · Journal of Investigative Dermatology · doi:10.1111/1523-1747.ep12531034 · Cited by 352
Plasma ceramide and lysophosphatidylcholine inversely correlate with mortality in sepsis patients
Drobnik, W., Liebisch, G., Audebert, F., Fröhlich, D., Glück, T., Vogel, P. M., Rothe, G., & Schmitz, G. (2003) · Journal of Lipid Research · doi:10.1194/jlr.m200401-jlr200 · Cited by 350
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Tsugawa, H., Ikeda, K., Takahashi, M., Satoh, A., Mori, Y., Uchino, H., Okahashi, N., Yamada, Y., Tada, I., Bonini, P., et al. (2020) · Nature Biotechnology · doi:10.1038/s41587-020-0531-2 · Cited by 895
The important role of stratum corneum lipids for the cutaneous barrier function
van Smeden, J., Janssens, M., Gooris, G. S., & Bouwstra, J. A. (2014) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2013.11.006 · Cited by 549
Very long-chain fatty acids: elongation, physiology and related disorders
Kihara, A. (2012) · The Journal of Biochemistry · doi:10.1093/jb/mvs105 · Cited by 453
Changes in the Ceramide Profile of Atopic Dermatitis Patients
Ishikawa, J., Narita, H., Kondo, N., Hotta, M., Takagi, Y., Masukawa, Y., Kitahara, T., Takema, Y., Koyano, S., Yamazaki, S., et al. (2010) · Journal of Investigative Dermatology · doi:10.1038/jid.2010.161 · Cited by 372
Self-citationvan Smeden, J., & Bouwstra, J. A. (2016) · Current Problems in Dermatology · doi:10.1159/000441540 · Cited by 366
Metadata
- Authors
- Masukawa, Y., Narita, H., Shimizu, E., Kondo, N., Sugai, Y., Oba, T., Homma, R., Ishikawa, J., Takagi, Y., Kitahara, T., Takema, Y., & Kita, K.
- Affiliations
- Kao Corporation (Japan) (JP)
- Corresponding author
- Masukawa, Yoshinori
- Venue
- Journal of Lipid Research
- Publisher
- Elsevier BV
- Published
- 2008-07
- Volume
- 49
- Issue
- 7
- Pages
- 1466-1476
- ISSN
- 0022-2275 (ISSN-L)
- OpenAlex
- W2159423422
- Cited by
- 322
- References
- 52
- Citations by year
- 2026: 7 · 2025: 6 · 2024: 17 · 2023: 13 · 2022: 17 · 2021: 19 · 2020: 22 · 2019: 18 · 2018: 24 · 2017: 25 · 2016: 24 · 2015: 19 · 2014: 25 · 2013: 30 · 2012: 19
- FWCI
- 4.94
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Lipid Research (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Kao Corporation
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Sphingolipid Metabolism and Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advancements in Transdermal Drug Delivery
- Keywords
- ceramide, stratum corneum, skin barrier, dihydrosphingosine, keratinization, normal-phase chromatography, tandem mass spectrometry
- MeSH terms
- Ceramides, Humans, Skin, Mass Spectrometry, Molecular Structure
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