The DOI matches its Crossref record; 52 of the 52 references it cites were found in a registry or an index.
Norepinephrine Is Required for Leptin Effects on Gene Expression in Brown and White Adipose Tissue1
Abstract
Exogenous leptin enhances energy utilization in ob/ob mice by binding its hypothalamic receptor and selectively increasing peripheral fat oxidation. Leptin also increases uncoupling protein 1 (UCP1) expression in brown adipose tissue (BAT), but the neurotransmitter that mediates this effect has not been established. The present experiments sought to determine whether leptin regulates UCP1 expression in BAT and its own expression in white adipose tissue (WAT) through the long or short forms of leptin receptor and modulation of norepinephrine release. Mice lacking dopamine beta-hydroxylase (Dbh-/-), the enzyme responsible for synthesizing norepinephrine and epinephrine from dopamine, were treated with leptin (20 microg/g body weight/day) for 3 days before they were euthanized. UCP1 messenger RNA (mRNA) and protein expression were 5-fold higher in BAT from control (Dbh+/-) compared with Dbh-/- mice. Leptin produced a 4-fold increase in UCP1 mRNA levels in Dbh+/- mice but had no effect on UCP1 expression in Dbh-/-. The beta3-adrenergic agonist, CL-316,243 increased UCP1 expression and established that BAT from both groups of mice was capable of responding to beta-adrenergic stimulation. Similarly, exogenous leptin reduced leptin mRNA in WAT from Dbh+/- but not Dbh-/- mice. In separate experiments, leptin produced comparable reductions in food intake in both Dbh+/- and Dbh-/- mice, illustrating that norepinephrine is not required for leptin's effect on food intake. Lastly, db/db mice lacking the long form of the leptin receptor failed to increase UCP1 mRNA in response to exogenous leptin but increased UCP1 mRNA in response to CL-316,243. These studies establish that norepinephrine is required for leptin to regulate its own expression in WAT and UCP1 expression in BAT and indicate that these effects are likely mediated through the centrally expressed long form of the leptin receptor.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“In addition, leptin, released by white adipocytes, regulates energy balance by effects on the hypothalamus that lead to inhibition of food intake and increased thermogenesis via activation of the SNS, though to date there is limited evidence that leptin mediates thermogenesis via the SNS in humans 66–70.”
From Cellular bioenergetics as a target for obesity therapy (2010) · cites this paper as [67]
In the citing paper
“In addition, leptin, released by white adipocytes, regulates energy balance by effects on the hypothalamus that lead to inhibition of food intake and increased thermogenesis via activation of the SNS, though to date there is limited evidence that leptin mediates thermogenesis via the SNS in humans 66–70.”
From Cellular bioenergetics as a target for obesity therapy (2010) · cites this paper as [67]
Citations
Cites
Weight-Reducing Effects of the Plasma Protein Encoded by the obese Gene
Halaas, J. L., Gajiwala, K. S., Maffei, M., Cohen, S. L., Chait, B. T., Rabinowitz, D., Lallone, R. L., Burley, S. K., & Friedman, J. M. (1995) · Science · doi:10.1126/science.7624777 · Cited by 4,451
Effects of the obese Gene Product on Body Weight Regulation in ob / ob Mice
Pelleymounter, M. A., Cullen, M. J., Baker, M. B., Hecht, R., Winters, D., Boone, T., & Collins, F. (1995) · Science · doi:10.1126/science.7624776 · Cited by 4,017
A Cold-Inducible Coactivator of Nuclear Receptors Linked to Adaptive Thermogenesis
Puigserver, P., Wu, Z., Park, C. W., Graves, R., Wright, M., & Spiegelman, B. M. (1998) · Cell · doi:10.1016/s0092-8674(00)81410-5 · Cited by 3,864
Maffei, M., Halaas, J., Ravussin, E., Pratley, R. E., Lee, G. H., Zhang, Y., Fei, H., Kim, S., Lallone, R., Ranganathan, S., et al. (1995) · Nature Medicine · doi:10.1038/nm1195-1155 · Cited by 3,725
Identification and expression cloning of a leptin receptor, OB-R.
Tartaglia, L. A., Dembski, M., Weng, X., Deng, N., Culpepper, J., Devos, R., Richards, G. J., Campfield, L. A., Clark, F. T., Deeds, J., et al. (1995) · Cell · doi:10.1016/0092-8674(95)90151-5 · Cited by 3,496
Campfield, L. A., Smith, F. J., Guisez, Y., Devos, R., & Burn, P. (1995) · Science · doi:10.1126/science.7624778 · Cited by 3,243
Abnormal splicing of the leptin receptor in diabetic mice
Lee, G.-H., Proenca, R., Montez, J. M., Carroll, K. M., Darvishzadeh, J. G., Lee, J. I., & Friedman, J. M. (1996) · Nature · doi:10.1038/379632a0 · Cited by 2,327
Chen, H., Charlat, O., Tartaglia, L. A., Woolf, E. A., Weng, X., Ellis, S. J., Lakey, N. D., Culpepper, J., More, K. J., Breitbart, R. E., et al. (1996) · Cell · doi:10.1016/s0092-8674(00)81294-5 · Cited by 2,214
Thermogenic mechanisms in brown fat.
Nicholls, D. G., & Locke, R. M. (1984) · Physiological Reviews · doi:10.1152/physrev.1984.64.1.1 · Cited by 1,602
Mice lacking mitochondrial uncoupling protein are cold-sensitive but not obese
Enerbäck, S., Jacobsson, A., Simpson, E. M., Guerra, C., Yamashita, H., Harper, M.-E., & Kozak, L. P. (1997) · Nature · doi:10.1038/387090a0 · Cited by 1,379
Cited by
Sorted by citations
Items 101–107 of 107
Leptine et sommeil : des liens méconnus ?
Fabre, V., & Adrien, J. (2004) · Médecine du Sommeil · doi:10.1016/s1769-4493(04)70164-8
Münzberg, H., & Gettys, T. W. (2013) · Encyclopedia of Biological Chemistry · doi:10.1016/b978-0-12-378630-2.00351-0
Self-citationObesity alters the immune response to influenza virus infection: a mechanism for immune modulation
Smith, A. G. (2019) · Carolina Digital Repository (University of North Carolina at Chapel Hill) · doi:10.17615/8g6s-9d79
Exercise-induced alterations in melanocortin receptor expression and inflammation
Henagan, T. M. (2010) · Louisiana State University · doi:10.31390/gradschool_dissertations.1018
Ceglarek, V. M., Guareschi, Z. M. N. D. C., Soares, G. M., ECKER-PASSARELLO, R. C., Balbo, S. L., Bonfleur, M. L., & Grassiolli, S. (2020) · ABCD Arquivos Brasileiros de Cirurgia Digestiva (São Paulo) · doi:10.1590/0102-672020190001e1497
Characterisation of Functional Brown Adipose Tissue In Adult Humans
Orava, J. (2014) · UTUPub (University of Turku)
Gettys, T. W. (2004) · Encyclopedia of Biological Chemistry · doi:10.1016/b0-12-443710-9/00353-7
Self-citation
Metadata
- Authors
- Commins, S. P., Marsh, D. J., Thomas, S. A., Watson, P. M., Padgett, M. A., Palmiter, R., & Gettys, T. W.
- Affiliations
- Medical University of South Carolina (US)
- Howard Hughes Medical Institute (US)
- Corresponding author
- Gettys, Thomas W.
- Venue
- Endocrinology
- Publisher
- The Endocrine Society
- Published
- 1999-10
- Volume
- 140
- Issue
- 10
- Pages
- 4772-4778
- ISSN
- 0013-7227 (ISSN-L)
- 1945-7170
- OpenAlex
- W2051553082
- Cited by
- 107
- References
- 52
- Citations by year
- 2025: 1 · 2024: 1 · 2023: 1 · 2022: 1 · 2021: 2 · 2020: 4 · 2019: 5 · 2018: 3 · 2017: 4 · 2016: 2 · 2014: 3 · 2013: 7 · 2012: 2
- FWCI
- 3.60
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (7r01dk053981-04)
- National Institute of General Medical Sciences (GM 08716, T32GM008716)
- National Institute of Diabetes and Digestive and Kidney Diseases (DK 53981)
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (HD 09172)
- Topics
- Health Sciences › Medicine › Physiology (medical) › Adipose Tissue and Metabolism
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Regulation of Appetite and Obesity
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adipokines, Inflammation, and Metabolic Diseases
- Keywords
- leptin, norepinephrine, brown adipose tissue, white adipose tissue, UCP1, dopamine beta-hydroxylase, leptin receptor isoforms, beta3-adrenergic receptor, CL316,243, dietary intake, gene expression regulation, ob/ob mice, db/db mice
- MeSH terms
- Uncoupling Protein 1, Adipose Tissue, Animals, Body Weight, Adipose Tissue, Brown, Carrier Proteins, Diabetes Mellitus, Dopamine beta-Hydroxylase, Eating, Ion Channels, Male, Membrane Proteins, Mice, Inbred C57BL, Norepinephrine, Proteins, RNA, Messenger, Gene Expression, Mice, Knockout, Leptin, Mitochondrial Proteins, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
