Rates of Glucose Uptake in Adipose Tissue and Muscle in Vivo after a Mixed Meal in Women with Morbid Obesity
Abstract
BACKGROUND AND AIMS: Although whole-body insulin resistance in obesity is established, information on insulin action in peripheral tissues, especially adipose tissue (AD), is limited. This study was undertaken in morbid obesity to investigate insulin action on glucose disposal in AD and muscle (M). SUBJECTS AND METHODS: A meal was given to 30 obese (age 34 +/- 1 yr, body mass index 47 +/- 1 kg/m(2)) and 10 nonobese women (age 39 +/- 4 yr, body mass index 23 +/- 1 kg/m(2)). Samples for glucose and insulin were taken for 360 min from veins draining the abdominal subcutaneous AD and forearm muscles and from the radial artery. Blood flow (BF) was measured in AD ((133)Xe) and M (plethysmography). RESULTS: The area under the curve divided by time (AUC(0-360 min)/360 min) in obese vs. nonobese was as follows: 1) arterial glucose was similar 6.04 +/- 0.2 vs. 5.67 +/- 0.1 mm), but insulin was increased (65.5 +/- 6.6 vs. 28.7 +/- 1.7 mU/liter, P = 0.0004); 2) BF was decreased (3 +/- 0.2 vs. 4.4 +/- 0.3 ml/min per 100 ml tissue in M, P = 0.002 and 1.8 +/- 0.1 vs. 3.7 +/- 0.3 ml/min per 100 ml tissue in AD, P < 0.0001); 3) glucose uptake was decreased (0.9 +/- 0.1 vs. 2.3 +/- 0.4 micromol/min per 100 ml tissue in M, P = 0.002 and 0.45 +/- 0.1 vs. 1.1 +/- 0.17 micromol/min per 100 ml tissue in AD, P = 0.01); 4) fractional glucose extraction was decreased in M (5 +/- 1 vs. 9 +/- 1%, P = 0.03), but was similar in AD (3 +/- 1 vs. 3.6 +/- 1.4%); 5) glucose uptake (per total fat mass) was increased (0.275 +/- 0.04 vs. 0.12 +/- 0.02 mmol/min, P = 0.027). CONCLUSION: In morbid obesity, the sensitivity of glucose metabolism to insulin is impaired in M, due to defects in insulin-stimulated glucose use and decreased BF, and in AD, at least in part, due to decreased BF. However, increased total fat mass provides a sink for the excess of glucose and compensates for insulin resistance.
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In conditions such as IGT, T2DM or obesity, insulin-stimulated rates of blood flow in adipose tissue and muscle are severely impaired.
“In conditions such as IGT, T2DM or obesity, insulin-stimulated rates of blood flow in adipose tissue and muscle are severely impaired [54,55,56].”
From Effects of diet, lifestyle, Chrononutrition and alternative dietary interventions on postprandial Glycemia and insulin resistance (2022) · cites this paper as [56]
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- Authors
- Mitrou, P., Boutati, E., Lambadiari, V., Maratou, E., Papakonstantinou, A., Komesidou, V., Sidossis, L., Tountas, N., Katsilambros, N., Economopoulos, T., Raptis, S. A., & Dimitriadis, G.
- Affiliations
- National and Kapodistrian University of Athens (GR)
- Evangelismos Hospital (GR)
- Harokopio University of Athens (GR)
- Corresponding author
- Dimitriadis, George
- Venue
- The Journal of Clinical Endocrinology & Metabolism
- Publisher
- The Endocrine Society
- Published
- 2009-08
- Volume
- 94
- Issue
- 8
- Pages
- 2958-2961
- ISSN
- 0021-972X (ISSN-L)
- 1945-7197
- OpenAlex
- W2055854409
- Cited by
- 28
- References
- 21
- Citations by year
- 2026: 1 · 2025: 1 · 2022: 5 · 2021: 1 · 2020: 2 · 2019: 2 · 2018: 1 · 2017: 1 · 2016: 3 · 2015: 4 · 2014: 2 · 2013: 2
- FWCI
- 0.73
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The Journal of Clinical Endocrinology & Metabolism (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes, Cardiovascular Risks, and Lipoproteins
- Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Keywords
- morbid obesity, insulin resistance, glucose uptake, adipose tissue, skeletal muscle, mixed meal, glucose disposal, tissue blood flow, women, subcutaneous adipose tissue, forearm muscles, insulin action, peripheral insulin sensitivity, lipolysis, lipoprotein lipase, hepatic glucose production, hepatic insulin sensitivity, hepatic lipogenesis
- MeSH terms
- Adipose Tissue, Adult, Blood Glucose, Female, Glucose, Humans, Insulin, Obesity, Morbid, Muscle, Skeletal, Area Under Curve
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