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Cited by 36Thyroid Disorders and TreatmentsClinical Laboratory Practices and Quality ControlHormonal and reproductive studies

Measurement of Thyroxine Uptake from Serum by Cultured Human Hepatocytes as an Index of Thyroid Status: Reduced Thyroxine Uptake from Serum of Patients with Nonthyf oidal Illness*

SARNE, DAVID H., REFETOFF, SUMUEL·1985·The Journal of Clinical Endocrinology & Metabolism
DOI 10.1210/jcem-61-6-1046
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Abstract

The uptake of T4 from human serum by cultured human hepatoma cells, Hep G2, was compared with the free T4 estimates by equilibrium dialysis (DT4) and a resin uptake method (FT4I). The cellular uptake of T4 (CT4) was highly correlated with DT4 values (r = 0.97; P less than 0.0005) and FT4I values (r = 0.99; P less than 0.0005) in sera from euthyroid subjects and hypothyroid and thyrotoxic patients. In patients with abnormal levels of serum T4-binding globulin, the fractional uptake of T4 decreased as the TBG level increased, and the CT4 value remained within the normal range. Addition of increasing amounts of T4 to serum from a hypothyroid patient did not alter the relationships between CT4 and FT4I and between CT4 and DT4. DT4 and CT4 measurements were compared in sera from 13 patients with severe nonthyroidal illness (NTI). Six samples had DT4 values that clearly overestimated the cellular uptake, 2 patients had DT4 values in the thyrotoxic range but normal CT4 values, and 2 patients had DT4 values in the midnormal range and CT4 values below normal. When FT4I and CT4 values were compared in 19 NTI patients, 5 had FT4I values below the normal range and normal CT4 values. Overall, among patients with NTI, FT4I was low in 11 (58%), normal in 8 (42%), and high in 0; CT4 was low in 6 (32%), normal in 13 (68%), and high in 0, and DT4 was low in 2 (15%), normal in 9 (69%), and high in 2 (15%). In patients with NTI, the results of in vitro estimations of free T4 do not always correlate with the transfer of T4 to tissues, as assessed by T4 uptake in human hepatoma cells. In patients with NTI, equilibrium dialysis may overestimate and resin uptake measurements may underestimate the amount of T4 taken up by tissues. In some patients with severe NTI, tissue uptake of T4 is reduced.

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Crossref · 10.1210/jcem-61-6-1046

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  • Serum from patients with NTIS inhibits T4 uptake in rat hepatocytes, mediated in part by increased non-esterified fatty acids (NEFA) and bilirubin and by decreased albumin.

    “Using deiodination of T4 as a measure of cellular transport of T4 into rat hepatocytes, various older studies [4, 5, 6] reported that serum from patients with NTIS inhibits T4 uptake, mediated in part by increased non-esterified fatty acids (NEFA) and bilirubin and by decreased albumin.”

    From An update on non-thyroidal illness syndrome (2021) · cites this paper as [6]

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Authors
SARNE, D. H., & REFETOFF, S.
Affiliations
University of Chicago (US)
Venue
The Journal of Clinical Endocrinology & Metabolism
Publisher
The Endocrine Society
Published
1985-12
Volume
61
Issue
6
Pages
1046-1052
ISSN
  • 0021-972X (ISSN-L)
  • 1945-7197
DOI
10.1210/jcem-61-6-1046
OpenAlex
W2056176663
Cited by
36
References
29
Citations by year
2020: 1 · 2014: 1 · 2013: 1
FWCI
1.25
Type
journal article
Language
en
Open access
closed
Locations
  • The Journal of Clinical Endocrinology & Metabolism (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institutes of Health (AM 06169, AM 15070)
Topics
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Thyroid Disorders and Treatments
  • Health Sciences › Medicine › Physiology › Clinical Laboratory Practices and Quality Control
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Hormonal and reproductive studies
Keywords
thyroxine uptake, free thyroxine, equilibrium dialysis, low T3 syndrome, hypothyroidism, thyrotoxicosis, T4-binding globulin, HepG2 cells
MeSH terms
Cell Line, Child, Dialysis, Humans, Hyperthyroidism, Hypothyroidism, Liver, Reference Values, Thyroid Function Tests, Thyroxine, Thyroxine-Binding Proteins

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