The DOI matches its Crossref record; 48 of the 49 references it cites were found in a registry or an index.
Peripheral-Type Benzodiazepine Receptor-Mediated Action of Steroidogenic Acute Regulatory Protein on Cholesterol Entry into Leydig Cell Mitochondria
Open accessAbstract
Hormone-induced steroid biosynthesis begins with the transfer of cholesterol from intracellular stores into mitochondria. Steroidogenic acute regulatory protein (StAR) and peripheral-type benzodiazepine receptor (PBR) have been implicated in this rate-determining step of steroidogenesis. MA-10 mouse Leydig tumor cells were treated with and without oligodeoxynucleotides (ODNs) antisense to PBR and StAR followed by treatment with saturating concentrations of human choriogonadotropin. Treatment with ODNs antisense but not missense for both proteins inhibited the respective protein expression and the ability of the cells to synthesize steroids in response to human choriogonadotropin. Treatment of the cells with either ODNs antisense to PBR or a transducible peptide antagonist to PBR resulted in inhibition of the accumulation of the mature mitochondrial 30-kDa StAR protein, suggesting that the presence of PBR is required for StAR import into mitochondria. Addition of in vitro transcribed/translated 37-kDa StAR or a fusion protein of Tom20 (translocase of outer membrane) and StAR (Tom/StAR) to mitochondria isolated from control cells increased pregnenolone formation. Mitochondria isolated from cells treated with ODNs antisense, but not missense, to PBR failed to form pregnenolone and respond to either StAR or Tom/StAR proteins. Reincorporation of in vitro transcribed/translated PBR, but not PBR missing the cholesterol-binding domain, into MA-10 mitochondria rescued the ability of the mitochondria to form steroids and the ability of the mitochondria to respond to StAR and Tom/StAR proteins. These data suggest that both StAR and PBR proteins are indispensable elements of the steroidogenic machinery and function in a coordinated manner to transfer cholesterol into mitochondria.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The cited source says what the claim attributes to it.
In the citing paper
“StAR appears to interact with peripheral benzodiazepine receptor (84), voltage-dependent anion channel 1, and phosphate carrier protein (86), all proteins found on the OMM.”
From The Molecular Biology, Biochemistry, and Physiology of Human Steroidogenesis and Its Disorders (2011) · cites this paper as [84]
The cited source says what the claim attributes to it.
In the citing paper
“Substantial data indicate that the action of StAR also requires the translocator protein, TSPO (also known as the peripheral benzodiazepine receptor) on the OMM (83-85).”
From The Molecular Biology, Biochemistry, and Physiology of Human Steroidogenesis and Its Disorders (2011) · cites this paper as [84]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Bradford, M. M. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90527-3 · Cited by 255,302
Mosmann, T. (1983) · Journal of Immunological Methods · doi:10.1016/0022-1759(83)90303-4 · Cited by 56,104
Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
Elbashir, S. M., Harborth, J., Lendeckel, W., Yalcin, A., Weber, K., & Tuschl, T. (2001) · Nature · doi:10.1038/35078107 · Cited by 9,456
Molecular biology of steroid hormone synthesis
MILLER, W. L. (1988) · Endocrine Reviews · doi:10.1210/edrv-9-3-295 · Cited by 1,397
Self-citationClark, B. J., Wells, J., King, S. R., & Stocco, D. M. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)46930-x · Cited by 1,168
Regulation of the acute production of steroids in steroidogenic cells
STOCCO, D. M., & CLARK, B. J. (1996) · Endocrine Reviews · doi:10.1210/edrv-17-3-221 · Cited by 1,057
Role of steroidogenic acute regulatory protein in adrenal and gonadal steroidogenesis
Lin, D., Sugawara, T., Strauss, J. F., Clark, B. J., Stocco, D. M., Saenger, P., Rogol, A., & Miller, W. L. (1995) · Science · doi:10.1126/science.7892608 · Cited by 1,030
Self-citationThe pathophysiology and genetics of congenital lipoid adrenal hyperplasia
Bose, H. S., Sugawara, T., Strauss, J. F., & Miller, W. L. (1996) · New England Journal of Medicine · doi:10.1056/nejm199612193352503 · Cited by 676
Self-citationRegulation of the acute production of steroids in steroidogenic cells
Stocco, D. M. (1996) · Endocrine Reviews · doi:10.1210/er.17.3.221 · Cited by 635
Structure and lipid transport mechanism of a StAR-related domain
Tsujishita (2000) · Nature Structural Biology · doi:10.1038/75192 · Cited by 535
Enigma of the peripheral benzodiazepine receptor
Gavish, M., Bachman, I., Shoukrun, R., Katz, Y., Veenman, L., Weisinger, G., & Weizman, A. (1999) · Pharmacological Reviews · doi:10.1016/s0031-6997(24)01424-8 · Cited by 512
Peripheral benzodiazepine receptors and mitochondrial function
Casellas, P. (2002) · Neurochemistry International · doi:10.1016/s0197-0186(01)00118-8 · Cited by 455
Caron, K. M., Soo, S.-C., Wetsel, W. C., Stocco, D. M., Clark, B. J., & Parker, K. L. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.21.11540 · Cited by 449
PAPADOPOULOS, V. (1993) · Endocrine Reviews · doi:10.1210/edrv-14-2-222 · Cited by 417
Self-citationRapid regulation of steroidogenesis by mitochondrial protein import
Bose, H. S., Lingappa, V. R., & Miller, W. L. (2002) · Nature · doi:10.1038/417087a · Cited by 357
Self-citationKrueger, K. E., & Papadopoulos, V. (1990) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)77217-7 · Cited by 345
Self-citationLacapère, J.-J., & Papadopoulos, V. (2003) · Steroids · doi:10.1016/s0039-128x(03)00101-6 · Cited by 342
Self-citationLi, H., Yao, Z.-X., Degenhardt, B., Teper, G., & Papadopoulos, V. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.98.3.1267 · Cited by 317
Self-citationArakane, F., Sugawara, T., Nishino, H., Liu, Z., Holt, J. A., Pain, D., Stocco, D. M., Miller, W. L., & Strauss, J. F. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.24.13731 · Cited by 304
Self-citationHormonal and developmental regulation of the steroidogenic acute regulatory protein
Clark, B. J., Soo, S. C., Caron, K. M., Ikeda, Y., Parker, K. L., & Stocco, D. M. (1995) · Molecular Endocrinology · doi:10.1210/mend.9.10.8544843 · Cited by 303
The active form of the steroidogenic acute regulatory protein, StAR, appears to be a molten globule
Bose, H. S., Whittal, R. M., Baldwin, M. A., & Miller, W. L. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.13.7250 · Cited by 227
Self-citationPapadopoulos, V., Amri, H., Li, H., Boujrad, N., Vidić, B., & Garnier, M. J. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.51.32129 · Cited by 224
Self-citationArtemenko, I. P., Zhao, D., Hales, D. B., Hales, K. H., & Jefcoate, C. R. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m107815200 · Cited by 217
Self-citationMolecular control of luteal secretion of progesterone
NISWENDER, G. D. (2002) · Reproduction · doi:10.1530/rep.0.1230333 · Cited by 216
Steroidogenic Acute Regulatory Protein (StAR) Is A Sterol Transfer Protein
Kallen, C. B., Billheimer, J. T., Summers, S. A., Stayrook, S. E., Lewis, M., & Strauss, J. F. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.41.26285 · Cited by 215
Regulation by ACTH of steroid hormone biosynthesis in the adrenal cortex
Simpson, E. R., & Waterman, M. R. (1983) · Canadian Journal of Biochemistry and Cell Biology · doi:10.1139/o83-088 · Cited by 189
Delavoie, F., Li, H., Hardwick, M., Robert, J.-C., Giatzakis, C., Péranzi, G., Yao, Z.-X., Maccario, J., Lacapère, J.-J., & Papadopoulos, V. (2003) · Biochemistry · doi:10.1021/bi0267487 · Cited by 168
Self-citationHigh-flux mitochondrial cholesterol trafficking, a specialized function of the adrenal cortex
Jefcoate, C. R. E. (2002) · Journal of Clinical Investigation · doi:10.1172/jci16771 · Cited by 160
Garnier, M., Dimchev, A. B., Boujrad, N., Price, J. M., Musto, N. A., & Papadopoulos, V (1994) · Molecular Pharmacology · doi:10.1016/s0026-895x(25)09915-8 · Cited by 159
Self-citationStructural and Functional Study of Reconstituted Peripheral Benzodiazepine Receptor
Lacapère, J.-J., Delavoie, F., Li, H., Péranzi, G., Maccario, J., Papadopoulos, V., & Vidić, B. (2001) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.2001.4975 · Cited by 156
Self-citationHigh-flux mitochondrial cholesterol trafficking, a specialized function of the adrenal cortex
Jefcoate, C. R. E. (2002) · Journal of Clinical Investigation · doi:10.1172/jci0216771 · Cited by 141
Rispoli, L. A., Horvat, R. D., Roess, D. A., Barisas, B. G., Juengel, J. L., & NISWENDER, G. D. (2001) · Endocrinology · doi:10.1210/endo.142.1.8052 · Cited by 124
Strauss, J. F., Kallen, C. B., Christenson, L. K., Watari, H., Devoto, L., Arakane, F., Kiriakidou, M., & Sugawara, T. (1999) · PubMed · Cited by 123
In vitro studies on the role of the peripheral-type benzodiazepine receptor in steroidogenesis
Culty, M., Li, H., Boujrad, N., Amri, H., Vidic, B., Bernassau, J. M., Reversat, J. L., & Papadopoulos, V (1999) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/s0960-0760(99)00056-4 · Cited by 122
Self-citationProtein translocation pathways of the mitochondrion
Koehler, C. M. (2000) · FEBS Letters · doi:10.1016/s0014-5793(00)01664-1 · Cited by 109
Kletsas, D., Li, W., Han, Z. J., & Papadopoulos, V. (2004) · Biochemical Pharmacology · doi:10.1016/j.bcp.2004.01.021 · Cited by 107
Self-citationClark, B. J., Combs, R., Hales, K. H., Hales, D. B., & Stocco, D. M. (1997) · Endocrinology · doi:10.1210/endo.138.11.5535 · Cited by 104
Self-citationLi, H., & Papadopoulos, V. (1998) · Endocrinology · doi:10.1210/en.139.12.4991 · Cited by 94
Self-citationGranot, Z., Geiss‐Friedlander, R., Melamed‐Book, N., Eimerl, S., Timberg, R., Weiss, A. M., Hales, K. H., Hales, D. B., Stocco, D. M., & Orly, J. (2003) · Molecular Endocrinology · doi:10.1210/me.2003-0074 · Cited by 87
Self-citationChristensen, K. B., Bose, H. S., Harris, F. M., Miller, W. L., & Bell, J. D. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m100903200 · Cited by 68
Self-citationTuckey, R. C., Headlam, M. J., Bose, H. S., & Miller, W. L. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m206965200 · Cited by 67
Self-citationTrophic Stimulation of Steroidogenesis: In Search of the Elusive Trigger
Hall, P. F. (1985) · Elsevier eBooks · doi:10.1016/b978-0-12-571141-8.50005-6 · Cited by 64
Boujrad, N. (1996) · Endocrinology · doi:10.1210/en.137.12.5727 · Cited by 62
Boujrad, N., Gaillard, J. L., Garnier, M. J., & Papadopoulos, V. (1994) · Endocrinology · doi:10.1210/endo.135.4.7925120 · Cited by 61
Self-citationKing, S. R., Liu, Z., Soh, J., Eimerl, S., Orly, J., & Stocco, D. M. (1999) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/s0960-0760(98)00152-6 · Cited by 60
Wright, G. L., & Reichenbecher, V. E. (1999) · Experimental Cell Research · doi:10.1006/excr.1998.4331 · Cited by 39
Hauet, T., Han, Z. J., Wang, Y., Hameury, F., Jayle, C., Gibelin, H., Goujon, J. M., Eugène, M., & Papadopoulos, V. (2002) · Transplantation · doi:10.1097/00007890-200212150-00006 · Cited by 34
Self-citationGazouli, M., Han, Z. J., & Papadopoulos, V. (2002) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1124/jpet.102.039388 · Cited by 28
Self-citation
Research works printed but not linked: 2
- Allen, 2002, Effects of mitochondrial disruptors on Leydig cell steroidogenesis and the steroidogenic acute regulatory (StAR) protein., Biol Reprod, 66, 108
- Garnier, 1994, In vitro reconstitution of a functional peripheral-type benzodiazepine receptor., Mol Pharmacol, 45, 201
Cited by
Sorted by citations
The Molecular Biology, Biochemistry, and Physiology of Human Steroidogenesis and Its Disorders
Miller, W. L., & Auchus, R. J. (2011) · Endocrine Reviews · doi:10.1210/er.2010-0013 · Cited by 2,458
Self-citationCellular cholesterol trafficking and compartmentalization
Ikonen, E. (2008) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2336 · Cited by 1,506
Papadopoulos, V., Baraldi, M., Guilarte, T. R., Knudsen, T. B., Lacapère, J.-J., Lindemann, P., Norenberg, M. D., Nutt, D., Weizman, A., Zhang, M.-R., et al. (2006) · Trends in Pharmacological Sciences · doi:10.1016/j.tips.2006.06.005 · Cited by 1,389
Self-citationRupprecht, R., Papadopoulos, V., Rammes, G., Baghai, T. C., Fan, J., Akula, N., Groyer, G., Adams, D., & Schumacher, M. (2010) · Nature Reviews Drug Discovery · doi:10.1038/nrd3295 · Cited by 930
Self-citationHorvath, S. E., & Daum, G. (2013) · Progress in Lipid Research · doi:10.1016/j.plipres.2013.07.002 · Cited by 943
Metadata
- Authors
- Hauet, T., Yao, Z.-X., Bose, H. S., Wall, C. T., Han, Z., Li, W., Hales, D. B., Miller, W. L., Culty, M., & Papadopoulos, V.
- Affiliations
- Georgetown University Medical Center (US)
- Department of Biochemistry and Molecular Biology (T.H., Z.-X.Y., C.T.W., Z.H., W.L., M.C., V.P.) Georgetown University Medical Center, Washington, DC 20057;
- University of California, San Francisco (US)
- University of Illinois Chicago (US)
- Corresponding author
- Papadopoulos, Vassilios
- ORCID
- Venue
- Molecular Endocrinology
- Publisher
- The Endocrine Society
- Published
- 2005-02-01
- Volume
- 19
- Issue
- 2
- Pages
- 540-554
- ISSN
- 0888-8809 (ISSN-L)
- 1944-9917
- OpenAlex
- W1966541444
- Semantic Scholar
- ef7e3760661b92876753b5b3c2d4dc2ca286da88
- Cited by
- 252
- References
- 49
- Citations by year
- 2026: 1 · 2025: 9 · 2024: 8 · 2023: 6 · 2022: 8 · 2021: 7 · 2020: 6 · 2019: 7 · 2018: 5 · 2017: 6 · 2016: 14 · 2015: 17 · 2014: 18 · 2013: 18 · 2012: 16
- FWCI
- 5.70
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Molecular Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Georgetown University
- National Institutes of Health (1r01hd037032-01)
- National Institute of Diabetes and Digestive and Kidney Diseases (DK-37922, R01DK037922)
- National Institute of Child Health and Human Development
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (HD 37032)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Physical Sciences › Chemistry › Organic Chemistry › Steroid Chemistry and Biochemistry
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Cholesterol and Lipid Metabolism
- Keywords
- steroidogenic acute regulatory protein, translocator protein (TSPO), cholesterol transport, Leydig cells, mitochondrial protein import, steroidogenesis, pregnenolone synthesis, antisense oligodeoxynucleotides, choriogonadotropin, MA-10 cells
- MeSH terms
- Steroidogenic Acute Regulatory Protein, Animals, Cell Survival, Cholesterol, Dose-Response Relationship, Drug, Gonadotropins, Humans, Hydroxycholesterols, Kinetics, Leydig Cells, Ligands, Male, Mitochondria, Mutation, Oligonucleotides, Peptides, Phosphoproteins, Pregnenolone, Protein Binding, Receptors, GABA-A, Steroids, Time Factors, Transcription, Genetic, Transfection, Protein Biosynthesis, Immunoblotting, Blotting, Western, Oligonucleotides, Antisense, Cyclic AMP-Dependent Protein Kinases, Mutation, Missense, RNA, Small Interfering, Cell Line, Tumor, Mice, In Vitro Techniques
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
