The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
Characterization of the Cholesterol Recognition Amino Acid Consensus Sequence of the Peripheral-Type Benzodiazepine Receptor
Open accessAbstract
We previously defined a cholesterol recognition/interaction amino acid consensus sequence [CRAC: L/V-X (1-5)-Y-X (1-5)-R/K] in the carboxyl terminus of the peripheral-type benzodiazepine receptor (PBR), a high-affinity drug and cholesterol-binding protein present in the outer mitochondrial membrane protein. This protein is involved in the regulation of cholesterol transport into the mitochondria, the rate-determining step in steroid biosynthesis. Reconstituted wild-type recombinant PBR into proteoliposomes demonstrated high-affinity 2-chlorophenyl)-N-methyl-N-(1-methyl-propyl)-3-isoquinolinecarboxamide and cholesterol binding. In the present work, we functionally and structurally characterized this CRAC motif using reconstituted recombinant PBR and nuclear magnetic resonance. Deletion of the C-terminal domain of PBR and mutation of the highly conserved among all PBR amino acid sequences Y152 of the CRAC domain resulted in loss of the ability of mutant recPBR to bind cholesterol. Nuclear magnetic resonance analysis of a PBR C-terminal peptide (144-169) containing the CRAC domain indicated a helical conformation for the L144-S159 fragment. As a result of the side-chain distribution, a groove that could fit a cholesterol molecule is delineated, on one hand, by Y152, T148, and L144, and, on the other hand, by Y153, M149, and A145. The aromatic rings of Y152 and Y153 assigned as essential residues for cholesterol binding constitute the gate of the groove. Furthermore, the side chain of R156 may cap the groove by interacting with the sterol hydroxyl group. These results provide structural and functional evidence supporting the finding that the CRAC domain in the cytosolic carboxyl-terminal domain of PBR might be responsible for the uptake and translocation of cholesterol into the mitochondria.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
VMD: visual molecular dynamics
Humphrey, W., Dalke, A., & Schulten, K. (1996) · Journal of Molecular Graphics · doi:10.1016/0263-7855(96)00018-5 · Cited by 66,952
CHARMM: A program for macromolecular energy, minimization, and dynamics calculations
Brooks, B. R., Bruccoleri, R. E., Olafson, B. D., States, D. J., Swaminathan, S., & Karplus, M. (1983) · Journal of Computational Chemistry · doi:10.1002/jcc.540040211 · Cited by 14,944
All-Atom Empirical Potential for Molecular Modeling and Dynamics Studies of Proteins
MacKerell, A. D., Bashford, D., Bellott, M., Dunbrack, R. L., Evanseck, J. D., Field, M. J., Fischer, S., Gao, J., Guo, H., Ha, S., et al. (1998) · The Journal of Physical Chemistry B · doi:10.1021/jp973084f · Cited by 14,311
NMR of Proteins and Nucleic Acids
(2006) · Nuclear Magnetic Resonance · doi:10.1039/9781847555236-00336 · Cited by 5,863
How proteins adapt to a membrane–water interface
Killian, J. A., & von Heijne, G. (2000) · Trends in Biochemical Sciences · doi:10.1016/s0968-0004(00)01626-1 · Cited by 700
Enigma of the peripheral benzodiazepine receptor
Gavish, M., Bachman, I., Shoukrun, R., Katz, Y., Veenman, L., Weisinger, G., & Weizman, A. (1999) · Pharmacological Reviews · doi:10.1016/s0031-6997(24)01424-8 · Cited by 512
Merutka, G., Jane Dyson, H., & Wright, P. E. (1995) · Journal of Biomolecular NMR · doi:10.1007/bf00227466 · Cited by 466
Peripheral benzodiazepine receptors and mitochondrial function
Casellas, P. (2002) · Neurochemistry International · doi:10.1016/s0197-0186(01)00118-8 · Cited by 446
PAPADOPOULOS, V. (1993) · Endocrine Reviews · doi:10.1210/edrv-14-2-222 · Cited by 417
Self-citationThe peripheral-type benzodiazepine receptor is functionally linked to Leydig cell steroidogenesis
Papadopoulos, V, Mukhin, A. G., Costa, E., & Krueger, K. E. (1990) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)39661-9 · Cited by 346
Self-citation
Cited by
Rupprecht, R., Papadopoulos, V., Rammes, G., Baghai, T. C., Fan, J., Akula, N., Groyer, G., Adams, D., & Schumacher, M. (2010) · Nature Reviews Drug Discovery · doi:10.1038/nrd3295 · Cited by 930
Self-citationThe role of cholesterol in membrane fusion
Yang, S.-T., Kreutzberger, A. J. B., Lee, J., Kiessling, V., & Tamm, L. K. (2016) · Chemistry and Physics of Lipids · doi:10.1016/j.chemphyslip.2016.05.003 · Cited by 421
Rone, M. B., Fan, J., & Papadopoulos, V. (2009) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2009.03.001 · Cited by 399
Self-citationCellular sources of TSPO expression in healthy and diseased brain
Nutma, E., Ceyzériat, K., Amor, S., Tsartsalis, S., Millet, P., Owen, D. R., Papadopoulos, V., & Tournier, B. B. (2021) · European Journal of Nuclear Medicine and Molecular Imaging · doi:10.1007/s00259-020-05166-2 · Cited by 192
Self-citationMitochondrial cholesterol: mechanisms of import and effects on mitochondrial function
Martin, L. A., Kennedy, B. E., & Karten, B. (2016) · Journal of Bioenergetics and Biomembranes · doi:10.1007/s10863-014-9592-6 · Cited by 128
Metadata
- Authors
- Jamin, N., Neumann, J.-M., Ostuni, M. A., Vu, T. K. N., Yao, Z.-X., Murail, S., Robert, J.-C., Giatzakis, C., Papadopoulos, V., & Lacapère, J.-J.
- Venue
- Molecular Endocrinology
- Publisher
- The Endocrine Society
- Published
- 2005-03
- Volume
- 19
- Issue
- 3
- Pages
- 588-594
- ISSN
- 0888-8809 (ISSN-L)
- 1944-9917
- OpenAlex
- W2043864366
- Cited by
- 255
- References
- 25
- FWCI
- 1.97
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Characterization of the Cholesterol Recognition Amino Acid Consensus Sequence of the Peripheral-Type Benzodiazepine Receptor. https://app.cochat.ai/papers/10.1210/me.2004-0308
- The paper
- Jamin, N., Neumann, J.-M., Ostuni, M. A., Vu, T. K. N., Yao, Z.-X., Murail, S., Robert, J.-C., Giatzakis, C., Papadopoulos, V., & Lacapère, J.-J. (2005). Characterization of the Cholesterol Recognition Amino Acid Consensus Sequence of the Peripheral-Type Benzodiazepine Receptor. Molecular Endocrinology. https://doi.org/10.1210/me.2004-0308
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
