The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
Structure of Two in Tandem Human 17β-Hydroxysteroid Dehydrogenase Genes
Open accessAbstract
Two human 17 beta-hydroxysteroid dehydrogenase (17 beta-HSD) genes (h17 beta-HSDI and h17 beta-HSDII) included in tandem within an approximately 13 kilobase pair fragment were isolated from a genomic lambda EMBL3 DNA library using cDNA encoding human 17 beta-HSD (hpE2DH216) as probe. We have determined the complete exon and intron sequences of the two genes as well as their 5' and 3'-flanking regions. Human 17 beta-HSDII contains six exons and five short introns for a total length of 3250 base pairs. The exon sequence of h17 beta-HSDII is identical to the previously reported hpE2DH216 cDNA while the overlapping nucleotide sequences of the corresponding exons and introns of h17 beta-HSDI and h17 beta-HSDII show 89% homology. In addition, we have used the hpE2DH216 cDNA to demonstrate the widespread expression of 17 beta-HSD mRNAs in steroidogenic and peripheral target tissues. These new findings provide the basis for a better understanding of the molecular mechanisms involved in 17 beta-HSD deficiency and peripheral sex steroid metabolism.
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“In the late 1980s, three independent groups reported the cloning of its cDNA, the first of any human HSD (503-505).”
From The Molecular Biology, Biochemistry, and Physiology of Human Steroidogenesis and Its Disorders (2011) · cites this paper as [505]
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Metadata
- Authors
- Luu-The, V., Labrie, C., Simard, J., Lachance, Y., Zhao, H.-F., Couët, J., Leblanc, G., & Labrie, F.
- Affiliations
- Université Laval (CA)
- Venue
- Molecular Endocrinology
- Publisher
- The Endocrine Society
- Published
- 1990-02
- Volume
- 4
- Issue
- 2
- Pages
- 268-275
- ISSN
- 0888-8809 (ISSN-L)
- 1944-9917
- OpenAlex
- W2074285202
- Cited by
- 173
- References
- 27
- Citations by year
- 2024: 1 · 2018: 1 · 2017: 2 · 2015: 5 · 2014: 1
- FWCI
- 5.75
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Molecular Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Sexual Differentiation and Disorders
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Estrogen and related hormone effects
- Keywords
- 17β-hydroxysteroid dehydrogenase, tandem gene duplication, gene structure, exon-intron organization, 5' and 3' flanking regions, steroidogenic tissues, mRNA expression
- MeSH terms
- Amino Acid Sequence, Base Sequence, Chromosome Mapping, Genes, Humans, Molecular Sequence Data, RNA, Messenger, 17-Hydroxysteroid Dehydrogenases, Restriction Mapping, DNA Probes, Genomic Library
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