Cited by 73Wnt/β-catenin signaling in development and cancerDevelopmental Biology and Gene RegulationPlanarian Biology and Electrostimulation

Lrp6 is required for convergent extension during Xenopus gastrulation

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Tahinci, Emilios, Thorne, Curtis A., Franklin, Jeffrey L., Salic, Adrian, Christian, Kelly M., Lee, Laura A., Coffey, Robert J., Lee, Ethan·2007·Development

Abstract

Wnt signaling regulates β-catenin-mediated gene transcription and planar cell polarity (PCP). The Wnt co-receptor, Lrp6, is required for signaling along the β-catenin arm. We show that Lrp6 downregulation (by morpholino injection) or overexpression in Xenopus embryos disrupts convergent extension, a hallmark feature of Wnt/PCP components. In embryos with decreased Lrp6 levels, cells of the dorsal marginal zone (DMZ), which undergoes extensive cellular rearrangements during gastrulation, exhibit decreased length:width ratios, decreased migration, and increased numbers of transient cytoplasmic protrusions. We show that Lrp6 opposes Wnt11 activity and localizes to the posterior edge of migrating DMZ cells and that Lrp6 downregulation enhances cortical and nuclear localization of Dsh and phospho-JNK, respectively. Taken together, these data suggest that Lrp6 inhibits Wnt/PCP signaling. Finally, we identify the region of the Lrp6 protein with Wnt/PCP activity to a stretch of 36 amino acids, distinct from regions required for Wnt/β-catenin signaling. We propose a model in which Lrp6 plays a critical role in the switch from Wnt/PCP to Wnt/β-catenin signaling.

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  • LRP6 can regulate convergent extension movements.

    “However, a recent study has shown that LRP6 can regulate convergent extension movements76 and more studies to address this question will be required.”

    From Wnt signal transduction pathways (2008) · cites this paper as [76]

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Authors
Tahinci, E., Thorne, C. A., Franklin, J. L., Salic, A., Christian, K. M., Lee, L. A., Coffey, R. J., & Lee, E.
Affiliations
  • Vanderbilt University Medical Center (US)
  • Harvard University (US)
Corresponding author
Lee, Ethan
Venue
Development
Published
2007-11-15
Volume
134
Issue
22
Pages
4095-4106
ISSN
  • 0950-1991 (ISSN-L)
  • 1477-9129
OpenAlex
W2090723435
Cited by
73
References
52
Citations by year
2026: 2 · 2025: 4 · 2024: 1 · 2022: 3 · 2021: 1 · 2020: 1 · 2019: 6 · 2018: 3 · 2017: 4 · 2016: 1 · 2014: 4 · 2013: 8 · 2012: 4
FWCI
3.44
Type
journal article
Language
en
Open access
Locations
  • Development (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
  • HighWire Press Open Archive (submittedVersion)
Funders
  • Pew Charitable Trusts
  • National Institutes of Health (DK007563)
  • U.S. Public Health Service (U01 084239, P50 95103)
  • National Cancer Institute (CA46413, P50CA095103, U01CA084239, R01CA046413)
  • National Institute of Diabetes and Digestive and Kidney Diseases (T32DK007563)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Wnt/β-catenin signaling in development and cancer
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Planarian Biology and Electrostimulation
Keywords
LRP6, convergent extension, Xenopus gastrulation, planar cell polarity, Wnt signaling, Wnt11, dorsal marginal zone, DSH, JNK, β-catenin signaling, morpholino knockdown, cell migration
MeSH terms
Amino Acid Sequence, Animals, Cells, Cultured, Embryo, Nonmammalian, Humans, Mesoderm, Models, Biological, Molecular Sequence Data, Receptors, LDL, Tissue Distribution, Xenopus, Signal Transduction, Cell Polarity, Sequence Homology, Amino Acid, Gene Expression Regulation, Developmental, Body Patterning, Animals, Genetically Modified, Wnt Proteins, beta Catenin, Gastrulation, Low Density Lipoprotein Receptor-Related Protein-6

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