The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
Directional migration of neural crest cells in vivo is regulated by Syndecan-4/Rac1 and non-canonical Wnt signaling/RhoA
Open accessAbstract
Directed cell migration is crucial for development, but most of our current knowledge is derived from in vitro studies. We analyzed how neural crest (NC)cells migrate in the direction of their target during embryonic development. We show that the proteoglycan Syndecan-4 (Syn4) is expressed in the migrating neural crest of Xenopus and zebrafish embryos. Loss-of-function studies using an antisense morpholino against syn4 show that this molecule is required for NC migration, but not for NC induction. Inhibition of Syn4 does not affect the velocity of cell migration, but significantly reduces the directional migration of NC cells. Furthermore, we show that Syn4 and PCP signaling control the directional migration of NC cells by regulating the direction in which the cell protrusions are generated during migration. Finally, we perform FRET analysis of Cdc42, Rac and RhoA in vitro and in vivo after interfering with Syn4 and PCP signaling. This is the first time that FRET analysis of small GTPases has been performed in vivo. Our results show that Syn4 inhibits Rac activity, whereas PCP signaling promotes RhoA activity. In addition, we show that RhoA inhibits Rac in NC cells. We present a model in which Syn4 and PCP control directional NC migration by, at least in part,regulating membrane protrusions through the regulation of small GTPase activities.
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Cites
Cell Migration: A Physically Integrated Molecular Process
Lauffenburger, D. A., & Horwitz, A. F. (1996) · Cell · doi:10.1016/s0092-8674(00)81280-5 · Cited by 3,915
Taking Cell-Matrix Adhesions to the Third Dimension
Cukierman, E., Pankov, R., Stevens, D. R., & Yamada, K. M. (2001) · Science · doi:10.1126/science.1064829 · Cited by 3,027
RHO GTPASES: Biochemistry and Biology
Jaffe, A. B., & Hall, A. (2005) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.21.020604.150721 · Cited by 3,009
Rational design and characterization of a Rac GTPase-specific small molecule inhibitor
Gao, Y., Dickerson, J. B., Guo, F., Zheng, J., & Zheng, Y. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0307512101 · Cited by 1,091
Coactivation of Rac and Rho by Wnt/Frizzled signaling is required for vertebrate gastrulation
Habas, R., Dawid, I. B., & He, X. (2003) · Genes & Development · doi:10.1101/gad.1022203 · Cited by 603
Marlow, F., Topczewski, J., Sepich, D., & Solnica-Krezel, L. (2002) · Current Biology · doi:10.1016/s0960-9822(02)00864-3 · Cited by 337
Distinct functions of Rho and Rac are required for convergent extension during Xenopus gastrulation
Tahinci, E., & Symes, K. (2003) · Developmental Biology · doi:10.1016/s0012-1606(03)00206-9 · Cited by 162
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Minoux, M., & Rijli, F. M. (2010) · Development · doi:10.1242/dev.040048 · Cited by 507
Complement Fragment C3a Controls Mutual Cell Attraction during Collective Cell Migration
Carmona-Fontaine, C., Theveneau, E., Tzekou, A., Tada, M., Woods, M., Page, K. M., Parsons, M., Lambris, J. D., & Mayor, R. (2011) · Developmental Cell · doi:10.1016/j.devcel.2011.10.012 · Cited by 317
Self-citationLukoseviciute, M., Gavriouchkina, D., Williams, R. M., Hochgreb-Hagele, T., Senanayake, U., Chong-Morrison, V., Thongjuea, S., Repapi, E., Mead, A., & Sauka-Spengler, T. (2018) · Developmental Cell · doi:10.1016/j.devcel.2018.11.009 · Cited by 109
Auxiliary and Autonomous Proteoglycan Signaling Networks
Elfenbein, A., & Simons, M. (2010) · Methods in Enzymology · doi:10.1016/s0076-6879(10)80001-1 · Cited by 32
Metadata
- Authors
- Matthews, H. K., Marchant, L., Carmona-Fontaine, C., Kuriyama, S., Larraín, J., Holt, M. R., Parsons, M., & Mayor, R.
- Venue
- Development
- Publisher
- The Company of Biologists
- Published
- 2008-05-15
- Volume
- 135
- Issue
- 10
- Pages
- 1771-1780
- ISSN
- 0950-1991 (ISSN-L)
- 1477-9129
- OpenAlex
- W2102803142
- Cited by
- 267
- References
- 7
- FWCI
- 8.69
- Type
- journal article
- Language
- en
- Open access
- green
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