DNA damage and tissue repair: What we can learn from planaria
Open accessAbstract
Faithful renewal of aging and damaged tissues is central to organismal lifespan. Stem cells (SCs) generate the cellular progeny that replenish adult tissues across the body but this task becomes increasingly compromised over time. The age related decline in SC-mediated tissue maintenance is a multifactorial event that commonly affects genome integrity. The presence of DNA damage in SCs that are under continuous demand to divide poses a great risk for age-related disorders such as cancer. However, performing analysis of SCs with genomic instability and the DNA damage response during tissue renewal present significant challenges. Here we introduce an alternative experimental system based on the planaria flatworm Schmidtea mediterranea to address at the organismal level studies intersecting SC-mediated tissue renewal in the presence of genomic instability. Planaria have abundant SCs (neoblasts) that maintain high rates of cellular turnover and a variety of molecular tools have been developed to induce DNA damage and dissect how neoblasts respond to this stressor. S. mediterranea displays high evolutionary conservation of DNA repair mechanisms and signaling pathways regulating adult SCs. We describe genetically induced-DNA damage models and highlight body-wide signals affecting cellular decisions such as survival, proliferation, and death in the presence of genomic instability. We also discuss transcriptomic changes in the DNA damage response during injury repair and propose DNA repair as key component of tissue regeneration. Additional studies using planaria will provide insights about mechanisms regulating survival and growth of cells with DNA damage during tissue renewal and regeneration.
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Planarians achieve resistance to tumors through efficient DNA repair mechanisms.
“On the other hand, planarians also achieve resistance to tumors through a variety of means, such as efficient DNA repair mechanisms.313”
From Inflammation and aging: signaling pathways and intervention therapies (2023) · cites this paper as [313]
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Decreased neoblast progeny and increased cell death during starvation-induced planarian degrowth
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(Neo)blast from the past: new insights into planarian stem cell lineages
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A Novel ATM/TP53/p21-Mediated Checkpoint Only Activated by Chronic γ-Irradiation
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Regional signals in the planarian body guide stem cell fate in the presence of DNA instability
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SUMO in the DNA damage response
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Alternative flow cytometry strategies to analyze stem cells and cell death in planarians
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Self-citationREGIONAL DIFFERENCES IN THE GROWTH OF SKIN TRANSPLANTS
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SmedOB1 is Required for Planarian Homeostasis and Regeneration
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DrRad51 is required for chiasmata formation in meiosis in planarian Dugesia ryukyuensis
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Flagged first, then by citations
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Inflammation and aging: signaling pathways and intervention therapies
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“On the other hand, planarians also achieve resistance to tumors through a variety of means, such as efficient DNA repair mechanisms.(313) All of these mechanisms provide enlightenment for us to study human aging.”
BackgroundAlternative Animal Models of Aging Research
Holtze, S., Gorshkova, E. A., Braude, S. H., Cellerino, A., Dammann, P., Hildebrandt, T. B., Hoeflich, A., Hoffmann, S., Koch, P., Tozzini, E. T., et al. (2021) · Frontiers in Molecular Biosciences · doi:10.3389/fmolb.2021.660959 · Cited by 131 · see citing works
The ubiquitin codes in cellular stress responses
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Davidian, D. C. C., LeGro, M., Barghouth, P. G., Rojas, S., Ziman, B., Maciel, E. I., Ardell, D. H., Escobar, A. L., & Oviedo, N. J. (2022) · Journal of Cell Science · doi:10.1242/jcs.259304 · Cited by 9 · see citing works
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- Authors
- Barghouth, P. G., Thiruvalluvan, M., LeGro, M., & Oviedo, N. J.
- Affiliations
- University of California, Merced (US)
- Corresponding author
- Oviedo, Néstor J.
- Venue
- Seminars in Cell & Developmental Biology
- Publisher
- Elsevier BV
- Published
- 2019-03
- Volume
- 87
- Pages
- 145-159
- ISSN
- 1084-9521 (ISSN-L)
- PMC
- PMC7039696
- OpenAlex
- W2799832928
- Cited by
- 48
- References
- 146
- Citations by year
- 2026: 5 · 2025: 7 · 2024: 6 · 2023: 11 · 2022: 5 · 2021: 7 · 2020: 4 · 2019: 2 · 2018: 1
- FWCI
- 0.34
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Seminars in Cell and Developmental Biology (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- eScholarship (California Digital Library) (submittedVersion)
- Funders
- University of California Cancer Research Coordinating Committee (CRR-18-525108)
- National Cancer Institute and National Institute of General Medical Sciences of the National Institute of Health (CA176114, GM109372)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Planarian Biology and Electrostimulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Keywords
- DNA damage response, tissue regeneration, planaria, Schmidtea mediterranea, neoblasts, stem cells, genomic instability, aging, DNA repair, tissue renewal, injury repair, transcriptomics
- MeSH terms
- Animals, DNA Damage, Planarians, Regeneration, Stem Cells
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