The DOI matches its Crossref record; 41 of the 41 references it cites were found in a registry or an index.
Regulation of imprinted X-chromosome inactivation in mice by Tsix
Abstract
In mammals, X-chromosome inactivation is imprinted in the extra-embryonic lineages with paternal X chromosome being preferentially inactivated. In this study, we investigate the role of Tsix, the antisense transcript from the Xist locus, in regulation of Xist expression and X- inactivation. We show that Tsix is transcribed from two putative promoters and its transcripts are processed. Expression of Tsix is first detected in blastocysts and is imprinted with only the maternal allele transcribed. The imprinted expression of Tsix persists in the extra- embryonic tissues after implantation, but is erased in embryonic tissues. To investigate the function of Tsix in X- inactivation, we disrupted Tsix by insertion of an IRESβgeo cassette in the second exon, which blocked transcripts from both promoters. While disruption of the paternal Tsix allele has no adverse effects on embryonic development, inheritance of a disrupted maternal allele results in ectopic Xist expression and early embryonic lethality, owing to inactivation of both X chromosomes in females and single X chromosome in males. Further, early developmental defects of female embryos with maternal transmission of Tsix mutation can be rescued by paternal inheritance of the Xist deletion. These results provide genetic evidence that Tsix plays a crucial role in maintaining Xist silencing in cis and in regulation of imprinted X-inactivation in the extra- embryonic tissues.
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Cites
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
Chomczynski, P., & Sacchi, N. (1987) · Analytical Biochemistry · doi:10.1016/0003-2697(87)90021-2 · Cited by 46,426
Targeted mutation of the DNA methyltransferase gene results in embryonic lethality
Li, E., Bestor, T. H., & Jaenisch, R. (1992) · Cell · doi:10.1016/0092-8674(92)90611-f · Cited by 3,984
Self-citationGene Action in the X-chromosome of the Mouse (Mus musculus L.)
LYON, M. F. (1961) · Nature · doi:10.1038/190372a0 · Cited by 3,978
Brown, C. J., Ballabio, A., Rupert, J. L., Lafreniere, R. G., Grompe, M., Tonlorenzi, R., & Willard, H. F. (1991) · Nature · doi:10.1038/349038a0 · Cited by 1,533
Requirement for Xist in X chromosome inactivation
Penny, G. D., Kay, G. F., Sheardown, S. A., Rastan, S., & Brockdorff, N. (1996) · Nature · doi:10.1038/379131a0 · Cited by 1,205
Requirement for Xist in X chromosome inactivation
(1996) · Trends in Genetics · doi:10.1016/0168-9525(96)81414-4 · Cited by 970
Tsix, a gene antisense to Xist at the X-inactivation centre
Lee, J., Davidow, L. S., & Warshawsky, D. (1999) · Nature Genetics · doi:10.1038/7734 · Cited by 829
TAKAGI, N., & SASAKI, M. (1975) · Nature · doi:10.1038/256640a0 · Cited by 791
Xist-deficient mice are defective in dosage compensation but not spermatogenesis.
Marahrens, Y., Panning, B., Dausman, J., Strauss, W., & Jaenisch, R. (1997) · Genes & Development · doi:10.1101/gad.11.2.156 · Cited by 734
Conservation of position and exclusive expression of mouse Xist from the inactive X chromosome
Brockdorff, N., Ashworth, A., Kay, G. F., Cooper, P., Smith, S., McCabe, V. M., Norris, D. P., Penny, G. D., Patel, D., & Rastan, S. (1991) · Nature · doi:10.1038/351329a0 · Cited by 643
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Fatica, A., & Bozzoni, I. (2014) · Nature Reviews Genetics · doi:10.1038/nrg3606 · Cited by 2,980
Chromatin modification and epigenetic reprogramming in mammalian development
Li, E. (2002) · Nature Reviews Genetics · doi:10.1038/nrg887 · Cited by 1,900
Self-citationMorris, K. V., & Mattick, J. S. (2014) · Nature Reviews Genetics · doi:10.1038/nrg3722 · Cited by 1,371
Nuclear reprogramming of somatic cells by in vitro hybridization with ES cells
Tada, M., Takahama, Y., Abe, K., Nakatsuji, N., & Tada, T. (2001) · Current Biology · doi:10.1016/s0960-9822(01)00459-6 · Cited by 889
X-Inactivation, Imprinting, and Long Noncoding RNAs in Health and Disease
Lee, J. T., & Bartolomei, M. S. (2013) · Cell · doi:10.1016/j.cell.2013.02.016 · Cited by 676
Rapid evolution of noncoding RNAs: lack of conservation does not mean lack of function
Pang, K. C., Frith, M. C., & Mattick, J. S. (2006) · Trends in Genetics · doi:10.1016/j.tig.2005.10.003 · Cited by 656
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Tian, D., Sun, S., & Lee, J. T. (2010) · Cell · doi:10.1016/j.cell.2010.09.049 · Cited by 522
X Chromosome Dosage Compensation: How Mammals Keep the Balance
Payer, B., & Lee, J. T. (2008) · Annual Review of Genetics · doi:10.1146/annurev.genet.42.110807.091711 · Cited by 521
YY1 Tethers Xist RNA to the Inactive X Nucleation Center
Jeon, Y., & Lee, J. T. (2011) · Cell · doi:10.1016/j.cell.2011.06.026 · Cited by 506
Xist RNA and the Mechanism of X Chromosome Inactivation
Plath, K., Mlynarczyk-Evans, S., Nusinow, D. A., & Panning, B. (2002) · Annual Review of Genetics · doi:10.1146/annurev.genet.36.042902.092433 · Cited by 450
Selective loss of imprinting in the placenta following preimplantation development in culture
Mann, M. R. W., Lee, S. S., Doherty, A. S., Verona, R. I., Nolen, L. D., Schultz, R. M., & Bartolomei, M. S. (2004) · Development · doi:10.1242/dev.01241 · Cited by 431
Runte, M. (2001) · Human Molecular Genetics · doi:10.1093/hmg/10.23.2687 · Cited by 391
Lessons from X-chromosome inactivation: long ncRNA as guides and tethers to the epigenome
Lee, J. T. (2009) · Genes & Development · doi:10.1101/gad.1811209 · Cited by 349
Jpx RNA Activates Xist by Evicting CTCF
Sun, S., Del Rosario, B. C., Szanto, A., Ogawa, Y., Jeon, Y., & Lee, J. T. (2013) · Cell · doi:10.1016/j.cell.2013.05.028 · Cited by 323
A Transient Heterochromatic State in Xist Preempts X Inactivation Choice without RNA Stabilization
Sun, B. K., Deaton, A. M., & Lee, J. T. (2006) · Molecular Cell · doi:10.1016/j.molcel.2006.01.028 · Cited by 290
CTCF, a Candidate Trans -Acting Factor for X-Inactivation Choice
Chao, W., Huynh, K. D., Spencer, R. J., Davidow, L. S., & Lee, J. T. (2002) · Science · doi:10.1126/science.1065982 · Cited by 262
The search for a marsupial XIC reveals a break with vertebrate synteny
Davidow, L. S., Breen, M., Duke, S. E., Samollow, P. B., McCarrey, J. R., & Lee, J. T. (2007) · Chromosome Research · doi:10.1007/s10577-007-1121-6 · Cited by 68
Metadata
- Authors
- Sado, T., Wang, Z., Sasaki, H., & Li, E.
- Venue
- Development
- Publisher
- The Company of Biologists
- Published
- 2001-04-15
- Volume
- 128
- Issue
- 8
- Pages
- 1275-1286
- ISSN
- 0950-1991 (ISSN-L)
- 1477-9129
- OpenAlex
- W2107011634
- Cited by
- 309
- References
- 41
- FWCI
- 11.73
- Type
- journal article
- Language
- en
- Open access
- closed
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