Student or educator? Sign up with your school email and get 6 months of Pro free.
CoChat
Sign Up
Cited by 77

The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.

Dissecting the role of PtdIns(4,5)P 2 in endocytosis and recycling of the transferrin receptor

Abe, Namiko, Inoue, Takanari, Galvez, Thierry, Klein, Lawrence, Meyer, Tobias·2008·Journal of Cell Science
DOI 10.1242/jcs.020792
Open this paper in CoChat — free account

Abstract

Endocytosis and recycling of membrane proteins are key processes for nutrient uptake, receptor signaling and synaptic transmission. Different steps in these fission and fusion cycles have been proposed to be regulated by physiological changes in plasma membrane (PM) phosphatidylinositol (4,5)-bisphosphate [PtdIns(4,5)P2] concentration. Here, we use a chemical enzyme-translocation strategy to rapidly reduce PM PtdIns(4,5)P2 levels while monitoring clathrin-mediated endocytosis and recycling. PtdIns(4,5)P2 hydrolysis blocked transferrin receptor endocytosis and led to a marked increase in the concentration of transferrin receptors in the PM, suggesting that endocytosis is more sensitive to changes in PtdIns(4,5)P2 than recycling. Reduction of PM PtdIns(4,5)P2 levels led to a near complete dissociation of Adaptor protein 2 (AP-2) from the PM but had only a small effect on clathrin assembly. This argues that receptor-mediated PtdIns(4,5)P2 reduction preferentially suppresses AP-2-mediated targeting of cargo to endocytic sites rather than the assembly of clathrin coats or recycling of endocytic vesicles.

Overview

Integrity signals

Registry

Crossref · 10.1242/jcs.020792

Cites

4 of 4 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Research works printed but not linked: 23

  1. Brodsky, F. M., Chen, C. Y., Knuehl, C., Towler, M. C. and Wakeham, D. E. (2001). Biological basket weaving: formation and function of clathrin-coated vesicles. Annu. Rev. Cell Dev. Biol.17, 517-568. · DOI · Paper page
  2. Boucrot, E., Saffarian, S., Massol, R., Kirchhausen, T. and Ehrlich, M. (2006). Role of lipids and actin in the formation of clathrin-coated pits. Exp. Cell Res.312, 4036-4048. · DOI
  3. Cremona, O. and De Camilli, P. (2001). Phosphoinositides in membrane traffic at the synapse. J. Cell Sci.114, 1041-1451. · DOI · Paper page
  4. Di Paolo, G., Moskowitz, H. S., Gipson, K., Wenk, M. R., Voronov, S., Obayashi, M., Flavell, R., Fitzsimonds, R. M., Ryan, T. A. and De Camilli, P. (2004). Impaired PtdIns(4,5)P2 synthesis in nerve terminals produces defects in synaptic vesicle trafficking. Nature431, 415-422. · DOI · Paper page
  5. Doughman, R. L., Firestone, A. J. and Anderson, R. A. (2003). Phosphatidylinositol phosphate kinases put PI4,5P(2) in its place. J. Membr. Biol.194, 77-89. · DOI · Paper page
  6. Gaidarov, I. and Keen, J. H. (1999). Phosphoinositide-AP-2 interactions required for targeting to plasma membrane clathrin-coated pits. J. Cell Biol.146, 755-764. · DOI · Paper page
  7. Gallusser, A. and Kirchhausen, T. (1993). The beta 1 and beta 2 subunits of the AP complexes are the clathrin coat assembly components. EMBO J.12, 5237-5244. · DOI
  8. Heo, W. D., Inoue, T., Park, W. S., Kim, M. L., Park, B. O., Wandless, T. J. and Meyer, T. (2006). PI(3,4,5)P3 and PI(4,5)P2 lipids target proteins with polybasic clusters to the plasma membrane. Science314, 1458-1461. · DOI · Paper page
  9. Hinrichsen, L., Harborth, J., Andrees, L., Weber, K. and Ungewickell, E. J. (2003). Effect of clathrin heavy chain- and alpha-adaptin-specific small inhibitory RNAs on endocytic accessory proteins and receptor trafficking in HeLa cells. J. Biol. Chem.278, 45160-45170. · DOI · Paper page
  10. Honing, S., Ricotta, D., Krauss, M., Spate, K., Spolaore, B., Motley, A., Robinson, M., Robinson, C., Haucke, V. and Owen, D. J. (2005). Phosphatidylinositol-(4,5)-bisphosphate regulates sorting signal recognition by the clathrin-associated adaptor complex AP2. Mol. Cell18, 519-531. · DOI · Paper page
  11. Kim, S., Kim, H., Chang, B., Ahn, N., Hwang, S., Di Paolo, G. and Chang, S. (2006). Regulation of transferrin recycling kinetics by PtdIns[4,5]P2 availability. FASEB J.20, 2399-2401. · DOI
  12. Krauss, M., Kinuta, M., Wenk, M. R., De Camilli, P., Takei, K. and Haucke, V. (2003). ARF6 stimulates clathrin/AP-2 recruitment to synaptic membranes by activating phosphatidylinositol phosphate kinase type Igamma. J. Cell Biol.162, 113-124. · DOI · Paper page
  13. Motley, A., Bright, N. A., Seaman, M. N. and Robinson, M. S. (2003). Clathrin-mediated endocytosis in AP-2-depleted cells. J. Cell Biol.162, 909-918. · DOI · Paper page
  14. Nichols, B. J. and Lippincott-Schwartz, J. (2001). Endocytosis without clathrin coats. Trends Cell Biol.11, 406-412. · DOI · Paper page
  15. Owen, D. J., Collins, B. M. and Evans, P. R. (2004). Adaptors for clathrin coats: structure and function. Annu. Rev. Cell Dev. Biol.20, 153-191. · DOI · Paper page
  16. Padron, D., Wang, Y. J., Yamamoto, M., Yin, H. and Roth, M. G. (2003). Phosphatidylinositol phosphate 5-kinase Ibeta recruits AP-2 to the plasma membrane and regulates rates of constitutive endocytosis. J. Cell Biol.162, 693-701. · DOI
  17. Roth, M. G. (2004). Phosphoinositides in constitutive membrane traffic. Physiol. Rev.84, 699-730. · DOI · Paper page
  18. Shih, W., Gallusser, A. and Kirchhausen, T. (1995). A clathrin-binding site in the hinge of the beta 2 chain of mammalian AP-2 complexes. J. Biol. Chem.270, 31083-31090. · DOI
  19. Stauffer, T. P., Ahn, S. and Meyer, T. (1998). Receptor-induced transient reduction in plasma membrane PtdIns(4,5)P2 concentration monitored in living cells. Curr. Biol.8, 343-346. · DOI · Paper page
  20. Suh, B. C. and Hille, B. (2005). Regulation of ion channels by phosphatidylinositol 4,5-bisphosphate. Curr. Opin. Neurobiol.15, 370-378. · DOI · Paper page
  21. Tsujishita, Y., Guo, S., Stolz, L. E., York, J. D. and Hurley, J. H. (2001). Specificity determinants in phosphoinositide dephosphorylation: crystal structure of an archetypal inositol polyphosphate 5-phosphatase. Cell105, 379-389. · DOI
  22. Varnai, P., Thyagarajan, B., Rohacs, T. and Balla, T. (2006). Rapidly inducible changes in phosphatidylinositol 4,5-bisphosphate levels influence multiple regulatory functions of the lipid in intact living cells. J. Cell Biol.175, 377-382. · DOI · Paper page
  23. Zoncu, R., Perera, R. M., Sebastian, R., Nakatsu, F., Chen, H., Balla, T., Ayala, G., Toomre, D. and De Camilli, P. V. (2007). Loss of endocytic clathrin-coated pits upon acute depletion of phosphatidylinositol 4,5-bisphosphate. Proc. Natl. Acad. Sci. USA104, 3793-3798. · DOI · Paper page

Cited by

See all 77

Metadata

Authors
Abe, N., Inoue, T., Galvez, T., Klein, L., & Meyer, T.
Affiliations
  • Graduate Program in Neurosciences, Stanford University School of Medicine, Stanford, CA 94305, USA
  • Department of Chemical and Systems Biology, Stanford University School of Medicine, Clark Center, 318 Campus Drive, Stanford, CA 94305, USA
  • Graduate Program in Biophysics, and Department of Microbiology and Immunology, Beckman Center, 279 Campus Drive, Stanford University Medical School, Stanford, CA 94305, USA
Venue
Journal of Cell Science
Publisher
The Company of Biologists
Published
2008-05-01
Volume
121
Issue
9
Pages
1488-1494
ISSN
  • 1477-9137
  • 0021-9533
DOI
10.1242/jcs.020792
Cited by
77
References
4
Language
en

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Data sources

Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.