Cited by 80Alzheimer's disease research and treatmentsMedicinal Plants and NeuroprotectionNeurobiology and Insect Physiology Research

Neuroprotective Effects of Limonene (+) against Aβ42-Induced Neurotoxicity in a Drosophila Model of Alzheimer’s Disease

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Shin, Myeongcheol, Liu, Quan Feng, Choi, Byoungyun, Shin, Changmin, Lee, Banseok, Yuan, Chunyu, Song, You Jin, Yun, Hye Sup +3 more·2020·Biological and Pharmaceutical Bulletin

Abstract

Forest bathing is suggested to have beneficial effects on various aspects of human health. Terpenes, isoprene based-phytochemicals emitted from trees, are largely responsible for these beneficial effects of forest bathing. Although the therapeutic effects of terpenes on various diseases have been revealed, their effects on neuronal health have not yet been studied in detail. Here, we screened 16 terpenes that are the main components of Korean forests using Drosophila Alzheimer's disease (AD) models to identify which terpenes have neuroprotective effects. Six out of the 16 terpenes, ρ-cymene, limonene (+), limonene (-), linalool, α-pinene (+), and β-pinene (-), partially suppressed the beta amyloid 42 (Aβ42)-induced rough eye phenotype when fed to Aβ42-expressing flies. Among them, limonene (+) restored the decreased survival of flies expressing Aβ42 in neurons during development. Limonene (+) treatment did not affect Aβ42 accumulation and aggregation, but did cause to decrease cell death, reactive oxygen species levels, extracellular signal-regulated kinase phosphorylation, and inflammation in the brains or the eye imaginal discs of Aβ42-expressing flies. This neuroprotective effect of limonene (+) was not associated with autophagic activity. Our results suggest that limonene (+) has a neuroprotective function against the neurotoxicity of Aβ42 and, thus, is a possible therapeutic reagent for AD.

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Research works printed but not linked: 2

  1. Lipsa D, Barrero-Moreno J, Coelhan M. Exposure to selected limo- nene oxidation products: 4-OPA, IPOH, 4-AMCH induces oxidative stress and inflammation in human lung epithelial cell lines. Chemo- sphere, 191, 937-945 (2018). · DOI
  2. Bosson A, Paumier A, Boisseau S, Jacquier-Sarlin M, Buisson A, Albrieux M. TRPA1 channels promote astrocytic Ca 2+ hyperac- tivity and synaptic dysfunction mediated by oligomeric forms of amyloid-β peptide. Mol. Neurodegener., 12, 53 (2017). · DOI

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Authors
Shin, M., Liu, Q. F., Choi, B., Shin, C., Lee, B., Yuan, C., Song, Y. J., Yun, H. S., Lee, I.-S., Koo, B.-S., & Cho, K. S.
Affiliations
  • Konkuk University (KR)
  • Dongguk University (KR)
  • Department of Biological Sciences, Konkuk University
Venue
Biological and Pharmaceutical Bulletin
Published
2020-03-01
Volume
43
Issue
3
Pages
409-417
ISSN
  • 0918-6158 (ISSN-L)
  • 1347-5215
OpenAlex
W2997755712
Cited by
80
References
58
Citations by year
2026: 3 · 2025: 12 · 2024: 8 · 2023: 18 · 2022: 14 · 2021: 17 · 2020: 8
FWCI
6.68
Type
journal article
Language
en
Open access
Locations
  • Biological and Pharmaceutical Bulletin (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Research Foundation
  • Ministry of Science, ICT and Future Planning
  • Korea Health Industry Development Institute (HI18C0041)
  • National Research Foundation of Korea (NRF-2016M3C1B6928005)
Topics
  • Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
  • Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
Keywords
limonene, neuroprotection, Alzheimer's disease, amyloid-beta 42, terpenes, forest bathing, Drosophila model, rough eye phenotype, neuronal cell death, reactive oxygen species, ERK1/2 phosphorylation, neuroinflammation, eye imaginal disc, autophagy
MeSH terms
Limonene, Alzheimer Disease, Animals, Autophagy, Brain, Disease Models, Animal, Drosophila melanogaster, Neuroglia, Peptide Fragments, Phosphorylation, Survival, Terpenes, Amyloid beta-Peptides, Reactive Oxygen Species, Neuroprotective Agents, MAP Kinase Signaling System, Animals, Genetically Modified

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