Neuroprotective Effects of Limonene (+) against Aβ42-Induced Neurotoxicity in a Drosophila Model of Alzheimer’s Disease
Open accessAbstract
Forest bathing is suggested to have beneficial effects on various aspects of human health. Terpenes, isoprene based-phytochemicals emitted from trees, are largely responsible for these beneficial effects of forest bathing. Although the therapeutic effects of terpenes on various diseases have been revealed, their effects on neuronal health have not yet been studied in detail. Here, we screened 16 terpenes that are the main components of Korean forests using Drosophila Alzheimer's disease (AD) models to identify which terpenes have neuroprotective effects. Six out of the 16 terpenes, ρ-cymene, limonene (+), limonene (-), linalool, α-pinene (+), and β-pinene (-), partially suppressed the beta amyloid 42 (Aβ42)-induced rough eye phenotype when fed to Aβ42-expressing flies. Among them, limonene (+) restored the decreased survival of flies expressing Aβ42 in neurons during development. Limonene (+) treatment did not affect Aβ42 accumulation and aggregation, but did cause to decrease cell death, reactive oxygen species levels, extracellular signal-regulated kinase phosphorylation, and inflammation in the brains or the eye imaginal discs of Aβ42-expressing flies. This neuroprotective effect of limonene (+) was not associated with autophagic activity. Our results suggest that limonene (+) has a neuroprotective function against the neurotoxicity of Aβ42 and, thus, is a possible therapeutic reagent for AD.
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In a Drosophila model of AD, limonene counteracts Aβ1-42-induced neurotoxicity by decreasing ROS levels and preventing Erk phosphorylation.
“A recent study performed in a Drosophila model of AD has shown that limonene is able to counteract the Aβ1-42-induced neurotoxicity by decreasing ROS levels and hence preventing Erk phosphorylation.”
From Exploring the Therapeutic Potential of Phytochemicals in Alzheimer’s Disease: Focus on Polyphenols and Monoterpenes (2022) · cites this paper as Shin et al., 2020
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- Authors
- Shin, M., Liu, Q. F., Choi, B., Shin, C., Lee, B., Yuan, C., Song, Y. J., Yun, H. S., Lee, I.-S., Koo, B.-S., & Cho, K. S.
- Affiliations
- Konkuk University (KR)
- Dongguk University (KR)
- Department of Biological Sciences, Konkuk University
- ORCID
- Venue
- Biological and Pharmaceutical Bulletin
- Publisher
- Pharmaceutical Society of Japan
- Published
- 2020-03-01
- Volume
- 43
- Issue
- 3
- Pages
- 409-417
- ISSN
- 0918-6158 (ISSN-L)
- 1347-5215
- OpenAlex
- W2997755712
- Cited by
- 80
- References
- 58
- Citations by year
- 2026: 3 · 2025: 12 · 2024: 8 · 2023: 18 · 2022: 14 · 2021: 17 · 2020: 8
- FWCI
- 6.68
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Locations
- Biological and Pharmaceutical Bulletin (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Research Foundation
- Ministry of Science, ICT and Future Planning
- Korea Health Industry Development Institute (HI18C0041)
- National Research Foundation of Korea (NRF-2016M3C1B6928005)
- Topics
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
- Keywords
- limonene, neuroprotection, Alzheimer's disease, amyloid-beta 42, terpenes, forest bathing, Drosophila model, rough eye phenotype, neuronal cell death, reactive oxygen species, ERK1/2 phosphorylation, neuroinflammation, eye imaginal disc, autophagy
- MeSH terms
- Limonene, Alzheimer Disease, Animals, Autophagy, Brain, Disease Models, Animal, Drosophila melanogaster, Neuroglia, Peptide Fragments, Phosphorylation, Survival, Terpenes, Amyloid beta-Peptides, Reactive Oxygen Species, Neuroprotective Agents, MAP Kinase Signaling System, Animals, Genetically Modified
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