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Cited by 59Muscle Physiology and DisordersHormonal and reproductive studiesGrowth Hormone and Insulin-like Growth Factors

Androgens affect myogenesis in vitro and increase local igf-1 expression

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SCULTHORPE, NICK, SOLOMON, ANDREW M., SINANAN, ANDREA C. M., BOULOUX, PIERRE-MARC G., GRACE, FERGAL, LEWIS, MARK P.·2012·Medicine & Science in Sports & Exercise
DOI 10.1249/mss.0b013e318237c5c0
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Abstract

PURPOSE: The mechanism whereby anabolic androgens are associated with hypertrophy of skeletal muscle is incompletely understood but may involve an interaction with locally generated insulin-like growth factor (IGF) 1. The present investigation utilized a cell culture model of human skeletal muscle-derived cell maturation to test the hypothesis that androgens increase differentiation of human muscle precursor cells in vitro and to assess effects of androgen with or without IGF-1 on IGF-1 messenger RNA (mRNA) expression in human muscle precursor cells. METHODS: Differentiation of muscle-derived cells was induced under standard low-serum conditions. Cultures were then exposed to androgen (testosterone (T)) at 50, 100, and 500 nM or IGF-1 (10-50 ng·mL⁻¹). Immunocytochemistry and real-time polymerase chain reaction (RT-PCR) were used to assess effects of androgens and IGF-1 after 3- (early) or 7-d (late) muscle differentiation, respectively; RT-PCR was used to quantify the effects on androgen receptor expression. RESULTS: Under low-serum conditions, 3-d exposure to androgens or IGF-1 or both resulted in no significant increase in cellular myogenic commitment. After 7-d exposure, however, T and IGF-1 were both found to increase fusion index with no observable synergistic effect. T also increased IGF-1 mRNA generation (P < 0.0001), whereas exogenous IGF-1 (P < 0.001) reduced IGF-1 mRNA transcription relative to control. The T effect was reversible after treatment with flutamide, an androgen receptor antagonist. CONCLUSIONS: Both T and IGF-1 increase myogenic commitment after 7-d exposure to a differentiation medium. With T causing a concomitant increase in IGF-1 mRNA underpinning IGF-1 as a central mediator in the cellular pathways associated with muscle hypertrophy, including those affected by androgens. The novel system described has the potential for elucidating the pattern of growth factor effects associated with androgens in skeletal muscle.

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  • Testosterone can promote muscle fiber regeneration and repair by activating muscle satellites and increasing cellular insulin-like growth factor-1 (IGF-1) levels.

    “Additionally, testosterone can promote muscle fiber regeneration and repair by activating muscle satellites and increasing cellular insulin-like growth factor-1 (IGF-1) levels (142, 143).”

    From From mitochondria to sarcopenia: role of 17β-estradiol and testosterone (2023) · cites this paper as [143]

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Authors
SCULTHORPE, N., SOLOMON, A. M., SINANAN, A. C. M., BOULOUX, P.-M. G., GRACE, F., & LEWIS, M. P.
Affiliations
  • The Royal Free Hospital (GB)
  • University College London (GB)
  • Kingston University London (GB)
  • Loughborough University (GB)
ORCID
Venue
Medicine & Science in Sports & Exercise
Publisher
Ovid Technologies (Wolters Kluwer Health)
Published
2012-04
Volume
44
Issue
4
Pages
610-615
ISSN
0195-9131 (ISSN-L)
DOI
10.1249/mss.0b013e318237c5c0
OpenAlex
W2031532210
Cited by
59
References
23
Citations by year
2026: 3 · 2025: 4 · 2024: 1 · 2023: 4 · 2022: 8 · 2021: 5 · 2020: 5 · 2019: 4 · 2018: 2 · 2017: 5 · 2016: 4 · 2015: 5 · 2014: 3 · 2013: 6
FWCI
1.17
Type
journal article
Language
en
Open access
Licence
cc-by-nc-nd
Locations
  • Medicine & Science in Sports & Exercise (publishedVersion)
  • PubMed (publishedVersion)
  • FedUni ResearchOnline (Federation University Australia) (submittedVersion)
  • Loughborough University Institutional Repository (Loughborough University) (submittedVersion, cc-by-nc-nd)
  • UCL Discovery (University College London) (submittedVersion)
  • Loughborough University Research Repository (Loughborough University) (submittedVersion, cc-by-nc-nd)
  • University of Bedfordshire Repository (University of Bedfordshire) (submittedVersion)
Funders
Wellcome Trust
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Hormonal and reproductive studies
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Growth Hormone and Insulin-like Growth Factors
Keywords
androgens, testosterone, insulin-like growth factor I, myogenesis, skeletal muscle hypertrophy, muscle precursor cells, IGF-1 mRNA expression, androgen receptor, flutamide, cell fusion
MeSH terms
Adolescent, Adult, Cells, Cultured, Flutamide, Humans, Insulin-Like Growth Factor I, Male, Receptors, Androgen, Testosterone, Muscle, Skeletal, Muscle Development, Young Adult, Androgen Receptor Antagonists

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