The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
The antisense strand of small interfering RNAs directs histone methylation and transcriptional gene silencing in human cells
Open accessAbstract
To determine mechanistically how siRNAs mediate transcriptional gene silencing (TGS) in human cells, we have measured histone methylation at targeted promoters, the dependency on active transcription, and whether or not both strands of the siRNA are required for siRNA-mediated TGS. We report here that siRNA treatment increases both H3K9 and H3K27 methylation of the targeted EF1A promoter and that this increase is dependent on nuclear specific delivery of the siRNA. We also find that TGS can be directed by the antisense strand alone, and requires active transcription by RNA polymerase II in human cells as evidenced by sensitivity to α-amanatin. The observation of antisense strand-specific siRNA-mediated TGS of EF1A was substantiated by targeting the U3 region of the HIV-1 LTR/promoter. Furthermore, we show that the antisense strand of siRNA EF52 associates with the transiently expressed Flag-tagged DNMT3A, the targeted EF1A promoter, and trimethylated H3K27. The observations reported here implicate a functional link between siRNA-mediated targeting of genomic regions (promoters), RNA Pol II function, histone methylation, and DNMT3A and support a paradigm in which the antisense strands of siRNAs alone can direct sequence-specific transcriptional gene silencing in human cells.
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Cites
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Self-citationRNA-directed de novo methylation of genomic sequences in plants
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Small Interfering RNA-Induced Transcriptional Gene Silencing in Human Cells
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Self-citationARGONAUTE4 Control of Locus-Specific siRNA Accumulation and DNA and Histone Methylation
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Self-citationmiRNA‐dependent gene silencing involving Ago2‐mediated cleavage of a circular antisense RNA
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Metadata
- Authors
- WEINBERG, M. S., VILLENEUVE, L. M., EHSANI, A., AMARZGUIOUI, M., AAGAARD, L., CHEN, Z.-X., RIGGS, A. D., ROSSI, J. J., & MORRIS, K. V.
- Venue
- RNA
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2005-12-22
- Volume
- 12
- Issue
- 2
- Pages
- 256-262
- ISSN
- 1355-8382 (ISSN-L)
- 1469-9001
- OpenAlex
- W1970018160
- Cited by
- 275
- References
- 34
- FWCI
- 7.14
- Type
- journal article
- Language
- en
- Open access
- bronze
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- WEINBERG, M. S., VILLENEUVE, L. M., EHSANI, A., AMARZGUIOUI, M., AAGAARD, L., CHEN, Z.-X., RIGGS, A. D., ROSSI, J. J., & MORRIS, K. V. (2006). The antisense strand of small interfering RNAs directs histone methylation and transcriptional gene silencing in human cells. RNA. https://doi.org/10.1261/rna.2235106
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