Post-transcriptional regulation of microRNA expression
Abstract
microRNAs (miRNAs) are endogenous, noncoding ∼22-nucleotide RNA molecules that have recently emerged as fundamental, post-transcriptional regulators of cognate target gene expression. Many mammalian miRNAs are expressed in a tissue-specific manner, a phenomenon that has so far been attributed to transcriptional regulation. We here show by Northern blots and in situ hybridization experiments that the expression of mammalian miRNAs can be regulated at the post-transcriptional level. In particular, miR-138 is spatially restricted to distinct cell types, while its precursor, pre-miR-138-2, is ubiquitously expressed throughout all tissues analyzed. Furthermore, pre-miR-138-2 is exported from the nucleus to the cytoplasm, suggesting that cleavage of this pre-miRNA by Dicer is restricted to certain tissues and cell types. Thus, differential processing of pre-miRNAs might be an alternative mechanism to control miRNA function.
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A similar post-transcriptional event may also explain the expression pattern of other brain-enriched miRNAs such as miR-138.
“A similar post-transcriptional event may also explain the expression pattern of other brain-enriched miRNAs such as miR-138 (Obernosterer et al., 2006).”
From Identification of Dynamically Regulated MicroRNA and mRNA Networks in Developing Oligodendrocytes (2008) · cites this paper as Obernosterer et al., 2006
There is a precedent for post-transcriptional regulation of miRNA expression, although this has not been demonstrated for different miRNAs from the same transcript.
“There is a precedent for post-transcriptional regulation of miRNA expression [48,49], although this has not been demonstrated for different miRNAs from the same transcript.”
From MicroRNAs show a wide diversity of expression profiles in the developing and mature central nervous system (2007) · cites this paper as [48]
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Sempere, L. F., Freemantle, S., Pitha-Rowe, I., Moss, E., Dmitrovsky, E., & Ambros, V. (2004) · Genome Biology · doi:10.1186/gb-2004-5-3-r13 · Cited by 1,578 · see citing works
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- Calin, G.A., Liu, C.G., Sevignani, C., Ferracin, M., Felli, N., Dumitru, C.D., Shimizu, M., Cimmino, A., Zupo, S., Dono, M., et al. 2004. MicroRNA profiling reveals distinct signatures in B cell chronic lymphocytic leukemias. Proc. Natl. Acad. Sci. 101: 11755-11760.
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Maza, R. M., Barreda-Manso, M. A., Reigada, D., Silván, Á., Muñoz-Galdeano, T., Soto, A., del Águila, Á., & Nieto-Díaz, M. (2022) · Biomedicines · doi:10.3390/biomedicines10071559 · Cited by 12 · see citing works
MicroRNA‐138‐5p suppresses excitatory synaptic strength at the cerebellar input layer
Delvendahl, I., Daswani, R., Winterer, J., Germain, P., Uhr, N. M., Schratt, G., & Müller, M. (2025) · The Journal of Physiology · doi:10.1113/jp288019 · Cited by 3 · see citing works
- Authors
- Obernosterer, G., Leuschner, P. J. F., Alenius, M., & Martinez, J.
- Venue
- RNA
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2006-05-31
- Volume
- 12
- Issue
- 7
- Pages
- 1161-1167
- ISSN
- 1355-8382
- 1469-9001
- Cited by
- 391
- References
- 1
- Language
- en
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