The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.
Tethering of human Ago proteins to mRNA mimics the miRNA-mediated repression of protein synthesis
Open accessAbstract
MicroRNAs (miRNAs) are ~21-nt-long RNAs involved in regulating development, differentiation, and other processes in eukaryotes. In metazoa, nearly all miRNAs control gene expression by imperfectly base-pairing with the 3′slated region (3′f target mRNAs and repressing protein synthesis by an unknown mechanism. It is also unknown whether miRNA–mRNA duplexes containing mismatches and bulges provide specific features that are recognized by factors mediating the repression. miRNAs form part of ribonucleoprotein complexes, miRNPs, that contain Argonaute (Ago) and other proteins. Here we demonstrate that effects of miRNAs on translation can be mimicked in human HeLa cells by the miRNA-independent tethering of Ago proteins to the 3′ a reporter mRNA. Inhibition of protein synthesis occurred without a change in the reporter mRNA level and was dependent on the number, but not the position, of the hairpins tethering hAgo2 to the 3′hese findings indicate that a primary function of miRNAs is to guide their associated proteins to the mRNA.
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N6-methyladenosine-dependent regulation of messenger RNA stability
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Regulation of mRNA Translation and Stability by microRNAs
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Self-citationThe mechanism of eukaryotic translation initiation and principles of its regulation
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Metadata
- Authors
- PILLAI, R. S., ARTUS, C. G., & FILIPOWICZ, W.
- Venue
- RNA
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2004-08-30
- Volume
- 10
- Issue
- 10
- Pages
- 1518-1525
- ISSN
- 1355-8382 (ISSN-L)
- 1469-9001
- OpenAlex
- W2163298186
- Cited by
- 389
- References
- 36
- FWCI
- 7.59
- Type
- journal article
- Language
- en
- Open access
- bronze
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