The DOI matches its Crossref record; 14 of the 14 references it cites were found in a registry or an index.
Rapid 3D light-sheet microscopy with a tunable lens
Open accessAbstract
The in-vivo investigation of highly dynamic biological samples, for example the beating zebrafish heart, requires high-speed volume imaging techniques. Light-sheet microscopy is ideal for such samples as it records high-contrast images of entire planes within large samples at once. However, in order to obtain images of different planes, it has been necessary to move the sample relative to the fixed focal plane of the detection objective lens. This mechanical movement limits speed, precision and may be harmful to the sample. We have built a light-sheet microscope that uses remote focusing with an electrically tunable lens (ETL). Without moving specimen or objective we have thereby achieved flexible volume imaging at much higher speeds than previously reported. Our high-speed microscope delivers 3D snapshots of sensitive biological samples. As an example, we imaged 17 planes within a beating zebrafish heart at 510 frames per second, equivalent to 30 volume scans per second. Movements, shape changes and signals across the entire volume can be followed which has been impossible with existing reconstruction techniques.
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Cites
Optical Sectioning Deep Inside Live Embryos by Selective Plane Illumination Microscopy
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Self-citationSelective plane illumination microscopy techniques in developmental biology
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Self-citationFast multicolor 3D imaging using aberration-corrected multifocus microscopy
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Even fluorescence excitation by multidirectional selective plane illumination microscopy (mSPIM)
Huisken, J., & Stainier, D. Y. R. (2007) · Optics Letters · doi:10.1364/ol.32.002608 · Cited by 424
Self-citationOptically sectioned imaging by oblique plane microscopy
Dunsby, C. (2008) · Optics Express · doi:10.1364/oe.16.020306 · Cited by 348
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Fast two-layer two-photon imaging of neuronal cell populations using an electrically tunable lens
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Self-citationRam, S., Prabhat, P., Chao, J., Sally Ward, E., & Ober, R. J. (2008) · Biophysical Journal · doi:10.1529/biophysj.108.140392 · Cited by 259
An optical technique for remote focusing in microscopy
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Multilayer mounting enables long-term imaging of zebrafish development in a light sheet microscope
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Cited by
In vivo imaging of neural activity
Yang, W., & Yuste, R. (2017) · Nature Methods · doi:10.1038/nmeth.4230 · Cited by 406
Real-time volumetric microscopy of in vivo dynamics and large-scale samples with SCAPE 2.0
Voleti, V., Patel, K. B., Li, W., Perez Campos, C., Bharadwaj, S., Yu, H., Ford, C., Casper, M. J., Yan, R. W., Liang, W., et al. (2019) · Nature Methods · doi:10.1038/s41592-019-0579-4 · Cited by 320
Light-sheet microscopy: a tutorial
Olarte, O. E., Andilla, J., Gualda, E. J., & Loza-Alvarez, P. (2018) · Advances in Optics and Photonics · doi:10.1364/aop.10.000111 · Cited by 298
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Metadata
- Authors
- Fahrbach, F. O., Voigt, F. F., Schmid, B., Helmchen, F., & Huisken, J.
- Venue
- Optics Express
- Publisher
- Optica Publishing Group
- Published
- 2013-08-30
- Volume
- 21
- Issue
- 18
- Pages
- 21010
- ISSN
- 1094-4087 (ISSN-L)
- OpenAlex
- W1964574783
- Cited by
- 347
- References
- 14
- FWCI
- 16.13
- Type
- journal article
- Language
- en
- Open access
- gold
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- Fahrbach, F. O., Voigt, F. F., Schmid, B., Helmchen, F., & Huisken, J. (2013). Rapid 3D light-sheet microscopy with a tunable lens. Optics Express. https://doi.org/10.1364/oe.21.021010
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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