A Resonance Raman Method for the Rapid Detection and Identification of Algae in Water
Open accessAbstract
Resonance Raman spectra are reported for aqueous suspensions of nine clones of marine plankton algae representing three classes, five genera, and seven species. Spectra are obtained easily either directly from culture or from samples concentrated by sedimentation. Spectra taken at 488 or 457.9 nm are of high quality and are sufficiently distinct to differentiate clones at the algal class level, and possibly also at the genus level. Strongest peaks occur near 1527 and 1158 cm −1 associated with ν(c = c) and ν(c – c) of carotenoid pigments, but information is contained in the entire region between 900 and 3000 cm −1 due to associated overtone and combination bands which can be assigned along with fundamental vibrations. Chlorophyll peaks also are quite pronounced. Spectra obtained using rapid flow techniques match those taken using slurries in sealed tubes if low laser power is used. The sensitivity and rapidity of the technique suggest that it may be useful in remote sensing applications.
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When detecting Raman scattered light, Rayleigh and Tyndall scattering must first be eliminated, elucidating faint characteristic spectra comprising astaxanthin, chlorophyll a, chlorophyll b, chlorophyll c, lipids, fatty acids, proteins, carbohydrates, nucleic acids.
“When detecting Raman scattered light, Rayleigh and Tyndall scattering must first be eliminated, elucidating faint characteristic spectra comprising astaxanthin, chlorophyll a, chlorophyll b, chlorophyll c, lipids, fatty acids, proteins, carbohydrates, nucleic acids, [18,38-44] (Fig. 2 & Fig. 3).”
From Microalgal process-monitoring based on high-selectivity spectroscopy tools: status and future perspectives (2018) · cites this paper as [39]
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- Authors
- Brahma, S. K., Hargraves, P. E., Howard, W. F., & Nelson, W. H.
- Affiliations
- University of Rhode Island (US)
- Venue
- Applied Spectroscopy
- Publisher
- SAGE Publications
- Published
- 1983-01
- Volume
- 37
- Issue
- 1
- Pages
- 55-58
- ISSN
- 0003-7028 (ISSN-L)
- 1943-3530
- OpenAlex
- W4249064071
- Cited by
- 44
- References
- 15
- Citations by year
- 2023: 2 · 2022: 1 · 2021: 1 · 2020: 1 · 2018: 1 · 2017: 6 · 2015: 2 · 2014: 2 · 2013: 2 · 2012: 3
- FWCI
- 1.63
- Type
- journal article
- Language
- en
- Open access
- Locations
- Applied Spectroscopy (publishedVersion)
- Journal of Media Literacy Education (submittedVersion, cc-by)
- Funders
- U.S. Department of the Interior
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
- Physical Sciences › Earth and Planetary Sciences › Oceanography › Marine and coastal ecosystems
- Physical Sciences › Chemistry › Analytical Chemistry › Spectroscopy and Chemometric Analyses
- Keywords
- resonance Raman spectroscopy, algal identification, carotenoids, chlorophyll, overtone and combination bands, algal taxonomy, remote sensing of algae
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