A Portable Raman Acoustic Levitation Spectroscopic System for the Identification and Environmental Monitoring of Algal Cells
Abstract
We report the coupling of a portable Raman spectrometer to an acoustic levitation device to enable environmental monitoring and the potential taxonomic identification of microalgae. Spectra of living cells were recorded at 785 nm using a fiber-optic probe coupled to a portable Raman spectrometer. The spectra exhibit an excellent signal-to-noise ratio and clearly show bands from chlorophyll a and beta-carotene. Spectra of levitated photobleached microalgae clearly show a reduction in chlorophyll a concentration relative to beta-carotene after 10 min of exposure to a quartz halogen lamp. Spectra recorded from levitated nitrogen-limited cells also show a significant reduction in bands associated with chlorophyll a, as compared to nitrogen-replete cells. To investigate the diagnostic capability of the technique, four species of microalgae were analyzed. Good quality spectra of all four species were obtained showing varying ratios of beta-carotene to chlorophyll. The combination of an acoustic levitation device and a portable Raman spectrometer shows potential as a taxonomic and environmental monitoring tool with direct application to field studies in remote environments.
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When detecting Raman scattered light, Rayleigh and Tyndall scattering must first be eliminated, elucidating faint characteristic spectra comprising astaxanthin, chlorophyll a, chlorophyll b, chlorophyll c, lipids, fatty acids, proteins, carbohydrates, nucleic acids.
“When detecting Raman scattered light, Rayleigh and Tyndall scattering must first be eliminated, elucidating faint characteristic spectra comprising astaxanthin, chlorophyll a, chlorophyll b, chlorophyll c, lipids, fatty acids, proteins, carbohydrates, nucleic acids, [18,38-44] (Fig. 2 & Fig. 3).”
From Microalgal process-monitoring based on high-selectivity spectroscopy tools: status and future perspectives (2018) · cites this paper as [43]
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- Authors
- Wood, B. R., Heraud, P., Stojkovic, S., Morrison, D., Beardall, J., & McNaughton, D.
- Affiliations
- Monash University (AU)
- Corresponding author
- Wood, Bayden R.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2005-06-21
- Volume
- 77
- Issue
- 15
- Pages
- 4955-4961
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W1966768396
- Cited by
- 135
- References
- 21
- Citations by year
- 2026: 6 · 2025: 6 · 2024: 7 · 2023: 9 · 2022: 14 · 2021: 7 · 2020: 10 · 2019: 6 · 2018: 2 · 2017: 6 · 2016: 8 · 2015: 6 · 2014: 10 · 2013: 6 · 2012: 6
- FWCI
- 3.42
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Analytical Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Australian Synchrotron
- Australian Research Council
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Bio-sensing Technologies
- Physical Sciences › Chemistry › Spectroscopy › Spectroscopy and Laser Applications
- Keywords
- Raman spectroscopy, acoustic levitation, microalgae, chlorophyll a, beta-carotene, photobleaching, nitrogen limitation, portable Raman spectrometer, optical fiber probe, environmental monitoring, algal taxonomy
- MeSH terms
- Chlorophyta, Environmental Monitoring, Nitrogen, Pheophytins, Spectrum Analysis, Raman
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