The DOI matches its Crossref record; 10 of the 10 references it cites were found in a registry or an index.
Targeted Inhibition of miRNA Maturation with Morpholinos Reveals a Role for miR-375 in Pancreatic Islet Development
Open accessAbstract
Several vertebrate microRNAs (miRNAs) have been implicated in cellular processes such as muscle differentiation, synapse function, and insulin secretion. In addition, analysis of Dicer null mutants has shown that miRNAs play a role in tissue morphogenesis. Nonetheless, only a few loss-of-function phenotypes for individual miRNAs have been described to date. Here, we introduce a quick and versatile method to interfere with miRNA function during zebrafish embryonic development. Morpholino oligonucleotides targeting the mature miRNA or the miRNA precursor specifically and temporally knock down miRNAs. Morpholinos can block processing of the primary miRNA (pri-miRNA) or the pre-miRNA, and they can inhibit the activity of the mature miRNA. We used this strategy to knock down 13 miRNAs conserved between zebrafish and mammals. For most miRNAs, this does not result in visible defects, but knockdown of miR-375 causes defects in the morphology of the pancreatic islet. Although the islet is still intact at 24 hours postfertilization, in later stages the islet cells become scattered. This phenotype can be recapitulated by independent control morpholinos targeting other sequences in the miR-375 precursor, excluding off-target effects as cause of the phenotype. The aberrant formation of the endocrine pancreas, caused by miR-375 knockdown, is one of the first loss-of-function phenotypes for an individual miRNA in vertebrate development. The miRNA knockdown strategy presented here will be widely used to unravel miRNA function in zebrafish.
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Cites
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Cited by
MicroRNAs in metabolism and metabolic disorders
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miR-375 maintains normal pancreatic α- and β-cell mass
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Metadata
- Authors
- Kloosterman, W. P., Lagendijk, A. K., Ketting, R. F., Moulton, J. D., & Plasterk, R. H. A.
- Venue
- PLoS Biology
- Publisher
- Public Library of Science (PLoS)
- Published
- 2007-07-24
- Volume
- 5
- Issue
- 8
- Pages
- e203
- ISSN
- 1544-9173 (ISSN-L)
- 1545-7885
- OpenAlex
- W2083246499
- Cited by
- 464
- References
- 10
- FWCI
- 10.82
- Type
- journal article
- Language
- en
- Open access
- gold
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