Mi2β Is Required for γ-Globin Gene Silencing: Temporal Assembly of a GATA-1-FOG-1-Mi2 Repressor Complex in β-YAC Transgenic Mice
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Activation of γ-globin gene expression in adults is known to be therapeutic for sickle cell disease. Thus, it follows that the converse, alleviation of repression, would be equally effective, since the net result would be the same: an increase in fetal hemoglobin. A GATA-1-FOG-1-Mi2 repressor complex was recently demonstrated to be recruited to the -566 GATA motif of the (A)γ-globin gene. We show that Mi2β is essential for γ-globin gene silencing using Mi2β conditional knockout β-YAC transgenic mice. In addition, increased expression of (A)γ-globin was detected in adult blood from β-YAC transgenic mice containing a T>G HPFH point mutation at the -566 GATA silencer site. ChIP experiments demonstrated that GATA-1 is recruited to this silencer at day E16, followed by recruitment of FOG-1 and Mi2 at day E17 in wild-type β-YAC transgenic mice. Recruitment of the GATA-1-mediated repressor complex was disrupted by the -566 HPFH mutation at developmental stages when it normally binds. Our data suggest that a temporal repression mechanism is operative in the silencing of γ-globin gene expression and that either a trans-acting Mi2β knockout deletion mutation or the cis-acting -566 (A)γ-globin HPFH point mutation disrupts establishment of repression, resulting in continued γ-globin gene transcription during adult definitive erythropoiesis.
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Knockdown or KO of Mi-2β reactivates γ-globin genes in the β-YAC transgenic mice.
“Consistent with a role in HbF silencing, knockdown or KO of Mi-2 β reactivates γ -globin genes in the β -YAC transgenic mice (23, 24).”
From Corepressor-dependent silencing of fetal hemoglobin expression by BCL11A (2013) · cites this paper as [23]
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- Authors
- Costa, F. C., Fedosyuk, H., Chazelle, A. M., Neades, R. Y., & Peterson, K. R.
- Affiliations
- University of Kansas Medical Center (US)
- Corresponding author
- Peterson, Kenneth R.
- ORCID
- Peterson, Kenneth R.
- Venue
- PLoS Genetics
- Publisher
- Public Library of Science (PLoS)
- Published
- 2012-12-20
- Volume
- 8
- Issue
- 12
- Pages
- e1003155
- ISSN
- 1553-7390 (ISSN-L)
- 1553-7404
- PMC
- PMC3527334
- OpenAlex
- W2149659073
- Cited by
- 30
- References
- 43
- Citations by year
- 2019: 1 · 2018: 4 · 2017: 9 · 2016: 5 · 2015: 1 · 2014: 6 · 2013: 4
- FWCI
- 1.68
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- PLoS Genetics (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- KU ScholarWorks (The University of Kansas) (submittedVersion, cc-by)
- Funders
- National Institutes of Health (DK081290, HL067336)
- National Heart, Lung, and Blood Institute (HL067336, R56HL067336, R01HL067336)
- National Institute of Diabetes and Digestive and Kidney Diseases (R56DK081290, DK081290, R01DK081290)
- Topics
- Health Sciences › Medicine › Genetics › Hemoglobinopathies and Related Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Hemoglobin structure and function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Keywords
- GATA-1, FOG-1, fetal hemoglobin, conditional knockout mice, transgenic mice, definitive erythropoiesis, sickle cell disease, transcriptional repression, temporal gene regulation
- MeSH terms
- Anemia, Sickle Cell, Animals, Erythropoiesis, Fetal Hemoglobin, Mice, Transgenic, Nuclear Proteins, Transcription Factors, Chromosomes, Artificial, Yeast, Gene Expression Regulation, Developmental, Gene Silencing, Embryonic Development, GATA1 Transcription Factor, Mice, beta-Globins, gamma-Globins, Mi-2 Nucleosome Remodeling and Deacetylase Complex
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