Cited by 92Bacterial biofilms and quorum sensingPolysaccharides Composition and ApplicationsMarine Biology and Environmental Chemistry

In Vitro Antibiofilm Activity of an Exopolysaccharide from the Marine Thermophilic Bacillus licheniformis T14

Spanò, Antonio, Laganà, Pasqualina, Visalli, Giuseppa, Maugeri, Teresa L., Gugliandolo, Concetta·2016·Current Microbiology

Abstract

The development of antibiofilm strategies is of major interest in contrasting bacterial pathogenic biofilms. A novel fructose and fucose rich exopolysaccharide (EPS1-T14) produced by the recently described thermophilic Bacillus licheniformis T14, isolated from a shallow hydrothermal vent of Panarea Island (Eolian Island, Italy), was evaluated for its effects on biofilm formation by multiresistant clinical strains of Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus aureus. The antibiofilm activity of EPS1-T14 was assessed by microtiter plate assays and visualized by confocal laser scanning microscopic images. EPS1-T14, with molecular weight of 1000 kDa, reduced biofilm formation on abiotic surfaces without affecting bacterial vitality. The novel EPS1-T14 is a water-soluble, noncytotoxic exopolymer able to prevent biofilm formation and its use may represent a promising therapeutic strategy for combating bacterial biofilm-associated infections. EPS1-T14 as antiadhesive biomolecule could be useful for novel prospective in medical and nonmedical applications.

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Crossref · 10.1007/s00284-015-0981-9

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  • A possible in vitro mechanism behind antimicrobial activity of LAB-derived EPS may be related to their ability to interfere with formation of pathogen biofilms by disrupting cellular integrity and communications.

    “A possible in vitro mechanism behind the antimicrobial activity of LAB-derived EPS may be related to their ability to interfere with the formation of pathogens biofilms by disrupting cellular integrity and communications [146,147].”

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Authors
Spanò, A., Laganà, P., Visalli, G., Maugeri, T. L., & Gugliandolo, C.
Affiliations
University of Messina (IT)
Corresponding author
Gugliandolo, Concetta
Venue
Current Microbiology
Published
2016-01-11
Volume
72
Issue
5
Pages
518-528
ISSN
  • 0343-8651 (ISSN-L)
  • 1432-0991
OpenAlex
W2233049449
Cited by
92
References
56
Citations by year
2026: 5 · 2025: 10 · 2024: 11 · 2023: 13 · 2022: 12 · 2021: 12 · 2020: 8 · 2019: 9 · 2018: 5 · 2017: 4 · 2016: 3
FWCI
3.64
Type
journal article
Language
en
Open access
closed
Locations
  • Current Microbiology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
  • Physical Sciences › Engineering › Ocean Engineering › Marine Biology and Environmental Chemistry
Keywords
exopolysaccharides, antibiofilm activity, Bacillus licheniformis, thermophilic bacteria, marine hydrothermal vents, biofilm inhibition, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus, multidrug-resistant bacteria, biofilm-associated infections
MeSH terms
Bacillus licheniformis, Escherichia coli, Polysaccharides, Bacterial, Pseudomonas aeruginosa, Seawater, Staphylococcus aureus, Biofilms, Hydrothermal Vents

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