Cited by 77Plant Stress Responses and ToleranceSeed Germination and PhysiologyPlant biochemistry and biosynthesis

Overexpression of the Artemisia Orthologue of ABA Receptor, AaPYL9, Enhances ABA Sensitivity and Improves Artemisinin Content in Artemisia annua L

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Zhang, Fangyuan, Lu, Xu, Lv, Zongyou, Zhang, Ling, Zhu, Mengmeng, Jiang, Weiming, Wang, Guofeng, Sun, Xiaofen +1 more·2013·PLoS ONE

Abstract

The phytohormone abscisic acid (ABA) plays an important role in plant development and environmental stress response. In this study, we cloned an ABA receptor orthologue, AaPYL9, from Artemisia annua L. AaPYL9 is expressed highly in leaf and flower. AaPYL9 protein can be localized in both nucleus and cytoplasm. Yeast two-hybrid assay shows AaPYL9 can specifically interact with AtABI1 but not with AtABI2, AtHAB1 or AtHAB2. ABA can enhance the interaction between AaPYL9 and AtABI1 while AaPYL9-89 Pro→Ser and AaPYL9-116 His→Ala point mutations abolishes the interaction. BiFC assay shows that AaPYL9 interacts with AtABI1 in nucleus in planta. Transgenic Arabidopsis plants over-expressing AaPYL9 are more sensitive to ABA in the seed germination and primary root growth than wild type. Consistent with this, ABA report genes have higher expression in AaPYL9 overexpressing plants compared to wild type after ABA treatment. Moreover, overexpression of AaPYL9 in A. annua increases not only drought tolerance, but also artemisinin content after ABA treatment, with significant enhancement of the expression of key genes in artemisinin biosynthesis. This study provides a way to develop A. annua with high-yielding artemisinin and high drought resistance.

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Crossref · 10.1371/journal.pone.0056697

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  • ABA also modulates artemisinin biosynthesis through AabZIP1 and AaPLY9 transcription factors.

    “ABA also modulates artemisinin biosynthesis through AabZIP1 and AaPLY9 TFs (Zhang et al. 2013, 2015).”

    From Enhancing artemisinin content in and delivery from Artemisia annua: a review of alternative, classical, and transgenic approaches (2021) · cites this paper as Zhang et al. 2013

    The Results section reports that ABA treatment increased artemisinin-biosynthesis gene expression and artemisinin content in plants overexpressing AaPYL9. But it identifies AaPYL9 as an ABA receptor, not a transcription factor; the paper does not establish the AabZIP1 portion of the jointly cited sentence. The claim also spells AaPYL9 as “AaPLY9.” (LLM, conf=0.90)

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Authors
Zhang, F., Lu, X., Lv, Z., Zhang, L., Zhu, M., Jiang, W., Wang, G., Sun, X., & Tang, K.
Affiliations
Shanghai Jiao Tong University (CN)
Corresponding author
Tang, Kexuan
Venue
PLoS ONE
Published
2013-02-20
Volume
8
Issue
2
Pages
e56697
ISSN
1932-6203 (ISSN-L)
OpenAlex
W1964082165
Cited by
73
References
65
Citations by year
2026: 1 · 2025: 2 · 2024: 4 · 2023: 7 · 2022: 4 · 2021: 2 · 2020: 19 · 2019: 3 · 2018: 3 · 2017: 8 · 2016: 6 · 2015: 9 · 2014: 5
FWCI
8.05
Type
journal article
Language
en
Open access
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Figshare (submittedVersion, cc-by)
Funders
  • Fudan University
  • Arizona Biomedical Research Commission
  • Shanghai Leading Academic Discipline Project (2011AA100605)
  • National High-tech Research and Development Program
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Seed Germination and Physiology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
Keywords
abscisic acid receptor, Artemisia annua, artemisinin biosynthesis, ABA sensitivity, drought tolerance, transgenic Arabidopsis, seed germination, root growth, abscisic acid signaling
MeSH terms
Abscisic Acid, Carrier Proteins, Phosphoprotein Phosphatases, Plant Growth Regulators, Arabidopsis, Receptors, Cytoplasmic and Nuclear, Gene Expression Regulation, Plant, Plant Leaves, Plant Roots, Germination, Arabidopsis Proteins, Plants, Genetically Modified, Artemisia annua, Artemisinins, Intracellular Signaling Peptides and Proteins, Droughts, Plant Development

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