Genetic engineering of artemisinin biosynthesis: prospects to improve its production

Production of the antimalarial drug precursor artemisinic acid in engineered yeast

Ro, D.-K., Paradise, E. M., Ouellet, M., Fisher, K. J., Newman, K. L., Ndungu, J. M., Ho, K. A., Eachus, R. A., Ham, T. S., Kirby, J., et al. (2006) · Nature · doi:10.1038/nature04640 · Cited by 2,805 · see citing works

High-level semi-synthetic production of the potent antimalarial artemisinin

Paddon, C. J., Westfall, P. J., Pitera, D. J., Benjamin, K., Fisher, K., McPhee, D., Leavell, M. D., Tai, A., Main, A., Eng, D., et al. (2013) · Nature · doi:10.1038/nature12051 · Cited by 2,060 · see citing works

Engineering a mevalonate pathway in Escherichia coli for production of terpenoids

Martin, V. J. J., Pitera, D. J., Withers, S. T., Newman, J. D., & Keasling, J. D. (2003) · Nature Biotechnology · doi:10.1038/nbt833 · Cited by 1,720 · see citing works

Qinghaosu (Artemisinin): The Price of Success

White, N. J. (2008) · Science · doi:10.1126/science.1155165 · Cited by 954 · see citing works

Production of amorphadiene in yeast, and its conversion to dihydroartemisinic acid, precursor to the antimalarial agent artemisinin

Westfall, P. J., Pitera, D. J., Lenihan, J. R., Eng, D., Woolard, F. X., Regentin, R., Horning, T., Tsuruta, H., Melis, D. J., Owens, A. E., et al. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1110740109 · Cited by 726 · see citing works

T-DNA of Agrobacterium tumefaciens encodes an enzyme of cytokinin biosynthesis.

Akiyoshi, D. E., Klee, H. J., Amasino, R. M., Nester, E. W., & Gordon, M. P. (1984) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.81.19.5994 · Cited by 513 · see citing works

The Jasmonate-Responsive AP2/ERF Transcription Factors AaERF1 and AaERF2 Positively Regulate Artemisinin Biosynthesis in Artemisia annua L.

Yu, Z.-X., Li, J., Yang, C., Hu, W., Wang, L.-J., & Chen, X. (2011) · Molecular Plant · doi:10.1093/mp/ssr087 · Cited by 474 · see citing works

<i>Artemisia annua</i> L. (Asteraceae) trichome‐specific cDNAs reveal CYP71AV1, a cytochrome P450 with a key role in the biosynthesis of the antimalarial sesquiterpene lactone artemisinin

Teoh, K. H., Polichuk, D. R., Reed, D. W., Nowak, G., & Covello, P. S. (2006) · FEBS Letters · doi:10.1016/j.febslet.2006.01.065 · Cited by 409 · see citing works

Engineering Escherichia coli for production of functionalized terpenoids using plant P450s

Chang, M. C. Y., Eachus, R. A., Trieu, W., Ro, D.-K., & Keasling, J. D. (2007) · Nature Chemical Biology · doi:10.1038/nchembio875 · Cited by 364 · see citing works

High-Level Production of Amorpha-4,11-Diene, a Precursor of the Antimalarial Agent Artemisinin, in Escherichia coli

Tsuruta, H., Paddon, C. J., Eng, D., Lenihan, J. R., Horning, T., Anthony, L. C., Regentin, R., Keasling, J. D., Renninger, N. S., & Newman, J. D. (2009) · PLoS ONE · doi:10.1371/journal.pone.0004489 · Cited by 364 · see citing works

The Biosynthesis of Artemisinin (Qinghaosu) and the Phytochemistry of Artemisia annua L. (Qinghao)

Brown, G. D. (2010) · Molecules · doi:10.3390/molecules15117603 · Cited by 363 · see citing works

The Genetic Map of <i>Artemisia annua</i> L. Identifies Loci Affecting Yield of the Antimalarial Drug Artemisinin

Graham, I. A., Besser, K., Blumer, S., Branigan, C. A., Czechowski, T., Elias, L., Guterman, I., Harvey, D., Isaac, P. G., Khan, A. M., et al. (2010) · Science · doi:10.1126/science.1182612 · Cited by 348 · see citing works

Isolation and Characterization of AaWRKY1, an Artemisia annua Transcription Factor that Regulates the Amorpha-4,11-diene Synthase Gene, a Key Gene of Artemisinin Biosynthesis

Ma, D., Pu, G., Lei, C., Ma, L., Wang, H., Guo, Y., Chen, J., Du, Z., Wang, H., Li, G., et al. (2009) · Plant and Cell Physiology · doi:10.1093/pcp/pcp149 · Cited by 333 · see citing works

The Molecular Cloning of Artemisinic Aldehyde Δ11(13) Reductase and Its Role in Glandular Trichome-dependent Biosynthesis of Artemisinin in Artemisia annua

Zhang, Y., Teoh, K. H., Reed, D. W., Maes, L., Goossens, A., Olson, D. J. H., Ross, A. R. S., & Covello, P. S. (2008) · Journal of Biological Chemistry · doi:10.1074/jbc.m803090200 · Cited by 312 · see citing works

Cytokinin/auxin balance in crown gall tumors is regulated by specific loci in the T-DNA

Akiyoshi, D. E., Morris, R. O., Hinz, R., Mischke, B. S., Kosuge, T., Garfinkel, D. J., Gordon, M. P., & Nester, E. W. (1983) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.80.2.407 · Cited by 298 · see citing works

RNAi-mediated replacement of morphine with the nonnarcotic alkaloid reticuline in opium poppy

Allen, R. S., Millgate, A. G., Chitty, J. A., Thisleton, J., MILLER, J. A., Fist, A. J., Gerlach, W. L., & Larkin, P. J. (2004) · Nature Biotechnology · doi:10.1038/nbt1033 · Cited by 294 · see citing works

Optimization of the mevalonate-based isoprenoid biosynthetic pathway in Escherichia coli for production of the anti-malarial drug precursor amorpha-4,11-diene

Anthony, J. R., Anthony, L. C., Nowroozi, F., Kwon, G., Newman, J. D., & Keasling, J. D. (2009) · Metabolic Engineering · doi:10.1016/j.ymben.2008.07.007 · Cited by 292 · see citing works

Molecular Cloning, Expression, and Characterization of Amorpha-4,11-diene Synthase, a Key Enzyme of Artemisinin Biosynthesis in Artemisia annua L.

Mercke, P., Bengtsson, M., Bouwmeester, H. J., Posthumus, M. A., & Brodelius, P. E. (2000) · Archives of Biochemistry and Biophysics · doi:10.1006/abbi.2000.1962 · Cited by 283 · see citing works

Artemisinin, a Novel Antimalarial Drug: Biochemical and Molecular Approaches for Enhanced Production

Abdin, M. Z., Israr, M., Rehman, R. U., & Jain, S. K. (2003) · Planta Medica · doi:10.1055/s-2003-38871 · Cited by 252 · see citing works

Molecular cloning of an aldehyde dehydrogenase implicated in artemisinin biosynthesis in <i>Artemisia annua</i> This paper is one of a selection of papers published in a Special Issue from the National Research Council of Canada – Plant Biotechnology Institute.

Teoh, K. H., Polichuk, D. R., Reed, D. W., & Covello, P. S. (2009) · Botany · doi:10.1139/b09-032 · Cited by 251 · see citing works

Amorpha-4,11-diene synthase: cloning and functional expression of a key enzyme in the biosynthetic pathway of the novel antimalarial drug artemisinin

Wallaart, T. E., Bouwmeester, H. J., Hille, J., Poppinga, L., & Maijers, N. C. A. (2001) · Planta · doi:10.1007/s004250000428 · Cited by 242 · see citing works

Localization of enzymes of artemisinin biosynthesis to the apical cells of glandular secretory trichomes of Artemisia annua L.

Olsson, M. E., Olofsson, L. M., Lindahl, A.-L., Lundgren, A., Brodelius, M., & Brodelius, P. E. (2009) · Phytochemistry · doi:10.1016/j.phytochem.2009.07.009 · Cited by 241 · see citing works

Dissection of the phytohormonal regulation of trichome formation and biosynthesis of the antimalarial compound artemisinin in <i>Artemisia annua</i> plants

Maes, L., Van Nieuwerburgh, F. C. W., Zhang, Y., Reed, D. W., Pollier, J., Vande Casteele, S. R. F., Inzé, D., Covello, P. S., Deforce, D. L. D., & Goossens, A. (2011) · New Phytologist · doi:10.1111/j.1469-8137.2010.03466.x · Cited by 240 · see citing works

Expression of a chimeric farnesyl diphosphate synthase gene in Artemisia annua L. transgenic plants via Agrobacterium tumefaciens-mediated transformation

Chen, D.-H., Ye, H.-C., & Li, G.-F. (2000) · Plant Science · doi:10.1016/s0168-9452(00)00217-x · Cited by 225 · see citing works

Precursor Balancing for Metabolic Engineering of Lycopene Production in Escherichia coli

Farmer, W. R., & Liao, J. C. (2001) · Biotechnology Progress · doi:10.1021/bp000137t · Cited by 213 · see citing works

Expression of an exogenous isopentenyl diphosphate isomerase gene enhances isoprenoid biosynthesis in Escherichia coli

Kajiwara, S., Fraser, P. D., Kondô, K., & Misawa, N. (1997) · Biochemical Journal · doi:10.1042/bj3240421 · Cited by 210 · see citing works

Development of transgenic <i>Artemisia annua</i> (Chinese wormwood) plants with an enhanced content of artemisinin, an effective anti‐malarial drug, by hairpin‐RNA‐mediated gene silencing

Zhang, L., Jing, F., Li, F., Li, M., Wang, Y., Wang, G., Sun, X., & Tang, K. (2009) · Biotechnology and Applied Biochemistry · doi:10.1042/ba20080068 · Cited by 206 · see citing works

Identification of Intermediates and Enzymes Involved in the Early Steps of Artemisinin Biosynthesis in<i>Artemisia annua</i>

Bertea, C. M., Freije, J. R., van der Woude, H., Verstappen, F. W., Perk, L., Marquez, V., De Kraker, J.-W., Posthumus, M. A., Jansen, B. J., de Groot, A., et al. (2005) · Planta Medica · doi:10.1055/s-2005-837749 · Cited by 205 · see citing works

STRUCTURE AND REACTION OF ARTEANNUIN

Liu, J. M. (1979) · Acta Chimica Sinica · Cited by 191 · see citing works

Effect of light irradiation on hairy root growth and artemisinin biosynthesis of Artemisia annua L

Liu, C., Guo, C., Wang, Y., & Ouyang, F. (2002) · Process Biochemistry · doi:10.1016/s0032-9592(02)00165-6 · Cited by 183 · see citing works

In vivo transformations of dihydroartemisinic acid in Artemisia annua plants

Brown, G. D., & Sy, L.-K. (2004) · Tetrahedron · doi:10.1016/j.tet.2003.11.070 · Cited by 183 · see citing works

The Genetics of Artemisinin Content in Artemisia annua L. and the Breeding of High Yielding Cultivars

Delabays, N., Simonnet, X., & Gaudin, M. (2001) · Current Medicinal Chemistry · doi:10.2174/0929867013371635 · Cited by 178 · see citing works

Evidence of artemisinin production from IPP stemming from both the mevalonate and the nonmevalonate pathways

Towler, M. J., & Weathers, P. J. (2007) · Plant Cell Reports · doi:10.1007/s00299-007-0420-x · Cited by 170 · see citing works

Application of RNAi to confirm theobromine as the major intermediate for caffeine biosynthesis in coffee plants with potential for construction of decaffeinated varieties

Ogita, S., Uefuji, H., Morimoto, M., & Sano, H. (2004) · Plant Molecular Biology · doi:10.1007/s11103-004-0393-x · Cited by 160 · see citing works

Artemisia annua L.

Tang, W., & Eisenbrand, G. (1992) · Springer eBooks · doi:10.1007/978-3-642-73739-8_22 · Cited by 158 · see citing works

Artemisinin: current state and perspectives for biotechnological production of an antimalarial drug

Liu, C., Zhao, Y., & Wang, Y. (2006) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-006-0452-0 · Cited by 158 · see citing works

Generation of the potent anti-malarial drug artemisinin in tobacco

Farhi, M., Marhevka, E., Ben‐Ari, J., Algamas‐Dimantov, A., Liang, Z., Zeevi, V., Edelbaum, O., Spitzer‐Rimon, B., Abeliovich, H., Schwartz, B., et al. (2011) · Nature Biotechnology · doi:10.1038/nbt.2054 · Cited by 153 · see citing works

Expression of prokaryotic 1‐deoxy‐ d ‐xylulose‐5‐phosphatases in Escherichia coli increases carotenoid and ubiquinone biosynthesis

Harker, M., & Bramley, P. M. (1999) · FEBS Letters · doi:10.1016/s0014-5793(99)00360-9 · Cited by 152 · see citing works

A Concise Synthesis of (+)-Artemisinin

Zhu, C., & Cook, S. P. (2012) · Journal of the American Chemical Society · doi:10.1021/ja3061479 · Cited by 152 · see citing works

Artemisinin biosynthesis in growing plants of Artemisia annua. A 13CO2 study

Schramek, N., Wang, H., Römisch-Margl, W., Keil, B., Radykewicz, T., Winzenhörlein, B., Beerhues, L., Bacher, A., Rohdich, F., Gershenzon, J., et al. (2010) · Phytochemistry · doi:10.1016/j.phytochem.2009.10.015 · Cited by 150 · see citing works

Dried Whole Plant Artemisia annua as an Antimalarial Therapy

Elfawal, M. A., Towler, M. J., Reich, N. G., Golenbock, D. T., Weathers, P. J., & Rich, S. M. (2012) · PLoS ONE · doi:10.1371/journal.pone.0052746 · Cited by 128 · see citing works

Making artemisinin

Covello, P. S. (2008) · Phytochemistry · doi:10.1016/j.phytochem.2008.10.001 · Cited by 127 · see citing works

Production of the new antimalarial drug artemisinin in shoot cultures of Artemisia annua L.

Woerdenbag, H. J., Lüers, J. F. J., van Uden, W., Pras, N., Malingré, T. M., & Alfermann, A. W. (1993) · Plant Cell Tissue and Organ Culture (PCTOC) · doi:10.1007/bf00029850 · Cited by 120 · see citing works

Overexpression of the HMG‐CoA Reductase Gene Leads to Enhanced Artemisinin Biosynthesis in TransgenicArtemisia annuaPlants

Aquil, S., Husaini, A. M., Abdin, M. Z., & Rather, G. M. (2009) · Planta Medica · doi:10.1055/s-0029-1185775 · Cited by 119 · see citing works

Effects of Overexpression of the Endogenous Farnesyl Diphosphate Synthase on the Artemisinin Content in <i>Artemisia annua</i> L.

Han, J., Liu, B., Ye, H., Wang, H., Li, Z., & Li, G. (2006) · Journal of Integrative Plant Biology · doi:10.1111/j.1744-7909.2006.00208.x · Cited by 115 · see citing works

Effects of ipt gene expression on the physiological and chemical characteristics of Artemisia annua L.

Geng, S., Ma, M., Ye, H., Liu, B., Li, G., & Chong, K. (2001) · Plant Science · doi:10.1016/s0168-9452(00)00453-2 · Cited by 110 · see citing works

Over-expression of HMG-CoA reductase and amorpha-4,11-diene synthase genes in Artemisia annua L. and its influence on artemisinin content

Alam, P., & Abdin, M. Z. (2011) · Plant Cell Reports · doi:10.1007/s00299-011-1099-6 · Cited by 108 · see citing works

Overexpression of farnesyl pyrophosphate synthase (FPS) gene affected artemisinin content and growth of Artemisia annua L.

Banyai, W., Kirdmanee, C., Mii, M., & Supaibulwatana, K. (2010) · Plant Cell, Tissue and Organ Culture (PCTOC) · doi:10.1007/s11240-010-9775-8 · Cited by 105 · see citing works

Metabolic fingerprinting investigation of Artemisia annua L. in different stages of development by gas chromatography and gas chromatography–mass spectrometry

Ma, C., Wang, H., Lu, X., Xu, G., & Liu, B. (2007) · Journal of Chromatography A · doi:10.1016/j.chroma.2007.09.023 · Cited by 103 · see citing works

Artemisinin: the biosynthetic pathway and its regulation in Artemisia annua, a terpenoid-rich species

Weathers, P. J., Elkholy, S., & Wobbe, K. K. (2006) · In Vitro Cellular &amp; Developmental Biology - Plant · doi:10.1079/ivp2006782 · Cited by 97 · see citing works

Cloning and analysis of a cDNA encoding farnesyl diphosphate synthase from Artemisia annua

Matsushita, Y., Kang, W., & Charlwood, B. V. (1996) · Gene · doi:10.1016/0378-1119(96)00054-6 · Cited by 95 · see citing works

Developing an industrial artemisinic acid fermentation process to support the cost‐effective production of antimalarial artemisinin‐based combination therapies

Lenihan, J. R., Tsuruta, H., Diola, D., Renninger, N. S., & Regentin, R. (2008) · Biotechnology Progress · doi:10.1002/btpr.27 · Cited by 85 · see citing works

Reproductive Development Modulates Gene Expression and Metabolite Levels with Possible Feedback Inhibition of Artemisinin in Artemisia annua

Arsenault, P. R., Vail, D. R., Wobbe, K. K., Erickson, K., & Weathers, P. J. (2010) · PLANT PHYSIOLOGY · doi:10.1104/pp.110.162552 · Cited by 85 · see citing works

Cloning of an Arabidopsis thaliana cDNA coding for farnesyl diphosphate synthase by functional complementation in yeast

Delourme, D., Lacroute, F., & Karst, F. (1994) · Plant Molecular Biology · doi:10.1007/bf00019499 · Cited by 83 · see citing works

Enhancement of artemisinin content by constitutive expression of the HMG-CoA reductase gene in high-yielding strain of Artemisia annua L.

Nafis, T., Akmal, M., Ram, M., Alam, P., Ahlawat, S., Mohd, A., & Abdin, M. Z. (2011) · Plant Biotechnology Reports · doi:10.1007/s11816-010-0156-x · Cited by 83 · see citing works

Metabolic engineering of artemisinin biosynthesis in Artemisia annua L.

Liu, B., Wang, H., Du, Z., Li, G., & Ye, H. (2010) · Plant Cell Reports · doi:10.1007/s00299-010-0967-9 · Cited by 82 · see citing works

Recent Advances in Artemisinin Production Through Heterologous Expression

Arsenault, P. R., Wobbe, K. K., & Weathers, P. J. (2008) · Current Medicinal Chemistry · doi:10.2174/092986708786242813 · Cited by 79 · see citing works

Trichome-specific expression of the amorpha-4,11-diene 12-hydroxylase (cyp71av1) gene, encoding a key enzyme of artemisinin biosynthesis in Artemisia annua, as reported by a promoter-GUS fusion

Wang, H., Han, J., Kanagarajan, S., Lundgren, A., & Brodelius, P. E. (2012) · Plant Molecular Biology · doi:10.1007/s11103-012-9986-y · Cited by 76 · see citing works

Terpenoid metabolic profiling analysis of transgenic Artemisia annua L. by comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry

Ma, C., Wang, H., Lu, X., Wang, H., Xu, G., & Liu, B. (2009) · Metabolomics · doi:10.1007/s11306-009-0170-6 · Cited by 76 · see citing works

Medicinal chemistry of bioactive natural products

Liang, X., & Fang, W. (2006) · doi:10.1002/0471739340 · Cited by 73 · see citing works

Overexpression of the Artemisia Orthologue of ABA Receptor, AaPYL9, Enhances ABA Sensitivity and Improves Artemisinin Content in Artemisia annua L

Zhang, F., Lu, X., Lv, Z., Zhang, L., Zhu, M., Jiang, W., Wang, G., Sun, X., & Tang, K. (2013) · PLoS ONE · doi:10.1371/journal.pone.0056697 · Cited by 73 · see citing works

Overexpression of the cytochrome P450 monooxygenase (cyp71av1) and cytochrome P450 reductase (cpr) genes increased artemisinin content in Artemisia annua (Asteraceae)

Shen, Q., Chen, Y. F., Wang, T., Wu, S. Y., Lu, X., Zhang, L., Zhang, F. Y., Jiang, W., & Tang, K. (2012) · Genetics and Molecular Research · doi:10.4238/2012.september.12.13 · Cited by 73 · see citing works

SELECTION AND BREEDING FOR HIGH ARTEMISININ (QINGHAOSU) YIELDING STRAINS OF ARTEMISIA ANNUA

Delabays, N., Collet, G. F., & Benakis, A. (1993) · Acta Horticulturae · doi:10.17660/actahortic.1993.330.24 · Cited by 72 · see citing works

Effect of Sterol Inhibitors on the Incorporation of¹⁴<i>C</i>-Isopentenyl Pyrophosphate into Artemisinin by a Cell-Free System from<i>Artemisia annua</i>Tissue Cultures and Plants

Kudakasseril, G., Lam, L., & Staba, E. (1987) · Planta Medica · doi:10.1055/s-2006-962706 · Cited by 70 · cited 1× · see citing works

Trichomes + roots + ROS = artemisinin: regulating artemisinin biosynthesis in Artemisia annua L.

Nguyen, K. T., Arsenault, P. R., & Weathers, P. J. (2011) · In Vitro Cellular & Developmental Biology - Plant · doi:10.1007/s11627-011-9343-x · Cited by 66 · see citing works

Ri-mediated transformation of Artemisia annua with a recombinant farnesyl diphosphate synthase gene for artemisinin production

Chen, D.-H., Liu, C.-J., Ye, H.-C., Li, G.-F., Liu, B.-Y., Meng, Y.-L., & Chen, X.-Y. (1999) · Plant Cell, Tissue and Organ Culture · doi:10.1023/a:1006326818509 · Cited by 65 · see citing works

Developmental and light‐regulated post‐translational control of 3‐hydroxy‐3‐methylglutaryl‐CoA reductase levels in potato

Korth, K. L., Jaggard, D. A. W., & Dixon, R. A. (2000) · The Plant Journal · doi:10.1046/j.1365-313x.2000.00821.x · Cited by 63 · see citing works

Bioconversion of arteannuin B to artemisinin

Nair, M. S. R., & Basile, D. V. (1993) · Journal of Natural Products · doi:10.1021/np50099a015 · Cited by 62 · cited 1× · see citing works

Artemisinin Biosynthesis Enhancement in Transgenic<i>Artemisia annua</i>Plants by Downregulation of the<i>β</i>-Caryophyllene Synthase Gene

Chen, J.-L., Fang, H.-M., Ji, Y.-P., Pu, G.-B., Guo, Y.-W., Huang, L.-L., Du, Z.-G., Liu, B.-Y., Ye, H.-C., Li, G.-F., et al. (2011) · Planta Medica · doi:10.1055/s-0030-1271038 · Cited by 61 · see citing works

The Molecular Cloning of Dihydroartemisinic Aldehyde Reductase and its Implication in Artemisinin Biosynthesis in<i>Artemisia annua</i>

Rydén, A.-M., Ruyter-Spira, C., Quax, W., Osada, H., Muranaka, T., Kayser, O., & Bouwmeester, H. (2010) · Planta Medica · doi:10.1055/s-0030-1249930 · Cited by 61 · see citing works

HMG-CoA reductase limits artemisinin biosynthesis and accumulation in Artemisia annua L. plants

Ram, M., Khan, M. A., Jha, P., Khan, S., Kiran, U., Ahmad, M. M., Javed, S., & Abdin, M. Z. (2010) · Acta Physiologiae Plantarum · doi:10.1007/s11738-010-0470-5 · Cited by 59 · see citing works

The Use of Combining Ability Analysis to Identify Elite Parents for Artemisia annua F1 Hybrid Production

Townsend, T., Segura, V., Chigeza, G., Penfield, T., Rae, A., Harvey, D., Bowles, D., & Graham, I. A. (2013) · PLoS ONE · doi:10.1371/journal.pone.0061989 · Cited by 56 · see citing works

Increased Accumulation of Artemisinin and Anthocyanins in Artemisia annua Expressing the Arabidopsis Blue Light Receptor CRY1

Hong, G., Hu, W., Li, J., Chen, X., & Wang, L.-J. (2009) · Plant Molecular Biology Reporter · doi:10.1007/s11105-008-0088-6 · Cited by 55 · see citing works

Hydroxylation of sesquiterpenes by enzymes from chicory (Cichorium intybus L.) roots

de Kraker, J., Schurink, M., Franssen, M. C. R., König, W. Α., de Groot, C. P. G. M., & Bouwmeester, H. J. (2003) · Tetrahedron · doi:10.1016/s0040-4020(02)01479-5 · Cited by 52 · see citing works

Isolation of high artemisinin-yielding clones of Artemisia annua

Jain, D. C., Mathur, A. K., Gupta, M. M., Singh, A. K., Verma, R. K., Gupta, A. P., & Kumar, S. (1996) · Phytochemistry · doi:10.1016/s0031-9422(96)00369-x · Cited by 51 · see citing works

Artemisinin combination therapies

Duffy, P. E., & Mutabingwa, T. K. (2006) · The Lancet · doi:10.1016/s0140-6736(06)68900-9 · Cited by 51 · see citing works

Farnesyl Pyrophosphate Synthase from White Lupin: Molecular Cloning, Expression, and Purification of the Expressed Protein

Attucci, S., Aitken, S. M., Gulick, P. J., & Ibrahim, R. K. (1995) · Archives of Biochemistry and Biophysics · doi:10.1006/abbi.1995.1422 · Cited by 46 · see citing works

Qinghaosu (Artemisinin)—A Fantastic Antimalarial Drug from a Traditional Chinese Medicine

Li, Y., Huang, H., & Wu, Y. (2005) · doi:10.1002/0471739340.ch5 · Cited by 45 · see citing works

Untitled work

(2012) · Plant Omics · Cited by 43 · see citing works

Biosynthesis of artemisinic acid in Artemisia annua

Akhila, A., Rani, K., & Thakur, R. S. (1990) · Phytochemistry · doi:10.1016/0031-9422(90)83020-2 · Cited by 41 · see citing works

Differentially Expressed Genes during Contrasting Growth Stages of Artemisia annua for Artemisinin Content

Nair, P., Misra, A., Singh, A., Shukla, A. K., Gupta, M. M., Gupta, A. K., Gupta, V., Khanuja, S. P. S., & Shasany, A. K. (2013) · PLoS ONE · doi:10.1371/journal.pone.0060375 · Cited by 38 · see citing works

Simulated digestion of dried leaves of Artemisia annua consumed as a treatment (pACT) for malaria

Weathers, P. J., Jordan, N. J., Lasin, P., & Towler, M. J. (2013) · Journal of Ethnopharmacology · doi:10.1016/j.jep.2013.11.043 · Cited by 30 · see citing works

Cloning and characterization of trichome-specific promoter of cpr71av1 gene involved in artemisinin biosynthesis in Artemisia annua L.

Wang, Y., Yang, K., Jing, F., Li, M., Deng, T., Huang, R.-Z., Wang, B., Wang, G., Sun, X., & Tang, K. (2011) · Molecular Biology · doi:10.1134/s0026893311040145 · Cited by 28 · see citing works

Co-overexpression of the HMGR and FPS genes enhances artemisinin content in Artemisia annua L.

Wang, Y., Jing, F., Yu, S., Chen, Y., Wang, T., Pin, L., Wang, G., Sun, X., & Tang, K. (2011) · Journal of Medicinal Plants Research · doi:10.5897/jmpr.9000369 · Cited by 27 · see citing works

Enzymatic Synthesis of Artemisinin from Natural and Synthetic Precursors

Bharel, S., Gulati, A., Abdin, M. Z., Srivastava, P. S., Vishwakarma, R. A., & Jain, S. K. (1998) · Journal of Natural Products · doi:10.1021/np970024s · Cited by 26 · see citing works

Molecular Cloning, Escherichia coli Expression and Genomic Organization of Squalene Synthase Gene from Artemisia annua

He, Y. (2003) · Cited by 26 · see citing works

Enhancement of Artemisinin Content through Four Cycles of Recurrent Selection with Relation to Heritability, Correlation and Molecular Marker inArtemisia annuaL.

Paul, S., Khanuja, S. P. S., Shasany, A. K., Gupta, M. M., Darokar, M. P., Saikia, D., & Gupta, A. K. (2010) · Planta Medica · doi:10.1055/s-0029-1240940 · Cited by 25 · see citing works

Enhanced artemisinin accumulation and metabolic profiling of transgenic Artemisia annua L. plants over-expressing by rate-limiting enzymes from isoprenoid pathway

Alam, P., Kamaluddin, K., Khan, M. A., Mohammad, A., Khan, R., & Abdin, M. Z. (2014) · Journal of Plant Interactions · doi:10.1080/17429145.2014.893030 · Cited by 18 · see citing works

Influence of Agrobacterium rhizogenes on induction of hairy roots for enhanced production of artemisinin in Artemisia annua L. plants

Ahlawat, S. (2012) · AFRICAN JOURNAL OF BIOTECHNOLOGY · doi:10.5897/ajb12.057 · Cited by 14 · see citing works

Isolation, characterization and structural studies of amorpha – 4, 11-diene synthase (ADS3963) from Arte a L. misia annu

Alam, P., Kiran, U., Ahmad, M. M., Kamaluddin, K., Khan, M. A., & Abdin, S. J. M. Z. (2010) · Bioinformation · doi:10.6026/97320630004421 · Cited by 11 · see citing works

. VARIATION AND HERITABILITY OF ARTEMISININ CONTENT IN ARTEMISIA ANNUA L.

Delabays, N., Darbellay, C., & Galland, N. (2010) · Taylor & Francis eBooks · doi:10.4324/9780203303061_chapter_11 · Cited by 4 · see citing works

INVITED REVIEW: ARTEMISININ: THE BIOSYNTHETIC PATHWAY AND ITS REGULATION IN ARTEMISIA ANNUA, A TERPENOID-RICH SPECIES

Weathers, P. J., Elkholy, S. F., & Wobbe, K. K. (2006) · Cited by 3 · see citing works

Engineering microbial cell factories for the production of plant natural products: from design principles to industrial-scale production

Liu, X., Ding, W., & Jiang, H. (2017) · Microbial Cell Factories · doi:10.1186/s12934-017-0732-7 · Cited by 146 · see citing works

JA-mediated transcriptional regulation of secondary metabolism in medicinal plants

Afrin, S., Huang, J., & Luo, Z.-Y. (2015) · 中国科学通报：英文版 · doi:10.1007/s11434-015-0813-0 · Cited by 132 · see citing works

Anti-Inflammatory Activity of Extracts and Pure Compounds Derived from Plants via Modulation of Signaling Pathways, Especially PI3K/AKT in Macrophages

Merecz-Sadowska, A., Sitarek, P., Śliwiński, T., & Zajdel, R. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21249605 · Cited by 90 · see citing works

Plantwide design and economic evaluation of two Continuous Pharmaceutical Manufacturing (CPM) cases: Ibuprofen and artemisinin

Jolliffe, H. G., & Gerogiorgis, D. I. (2016) · Computers & Chemical Engineering · doi:10.1016/j.compchemeng.2016.04.005 · Cited by 84 · see citing works

Enhancing artemisinin content in and delivery from Artemisia annua: a review of alternative, classical, and transgenic approaches

Wani, K. I., Choudhary, S., Zehra, A., Naeem, M., Weathers, P., & Aftab, T. (2021) · Planta · doi:10.1007/s00425-021-03676-3 · Cited by 74 · see citing works

More insights into the pharmacological effects of artemisinin

Zyad, A., Tilaoui, M., Jaafari, A., Oukerrou, M. A., & Mouse, H. A. (2017) · Phytotherapy Research · doi:10.1002/ptr.5958 · Cited by 72 · see citing works

Artemisinin biosynthesis in Artemisia annua and metabolic engineering: questions, challenges, and perspectives

Xie, D.-Y., Ma, D.-M., Judd, R., & Jones, A. L. (2016) · Phytochemistry Reviews · doi:10.1007/s11101-016-9480-2 · Cited by 63 · see citing works

Dual symbiosis between Piriformospora indica and Azotobacter chroococcum enhances the artemisinin content in Artemisia annua L.

Arora, M., Saxena, P., Choudhary, D. K., Abdin, M. Z., & Varma, A. (2016) · World Journal of Microbiology and Biotechnology · doi:10.1007/s11274-015-1972-5 · Cited by 59 · see citing works

Artemisinin attenuates doxorubicin induced cardiotoxicity and hepatotoxicity in rats

Aktaş, İ., Özmen, Ö., Tutun, H., Yalçın, A., & Türk, A. (2019) · Biotechnic & Histochemistry · doi:10.1080/10520295.2019.1647457 · Cited by 47 · see citing works

Recent Advances in the Synthetic Biology of Natural Drugs

Li, C.-Q., Lei, H.-M., Hu, Q.-Y., Li, G.-H., & Zhao, P. (2021) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2021.691152 · Cited by 46 · see citing works

Process modelling and simulation for continuous pharmaceutical manufacturing of artemisinin

Jolliffe, H. G., & Gerogiorgis, D. I. (2016) · Process Safety and Environmental Protection · doi:10.1016/j.cherd.2016.02.017 · Cited by 37 · see citing works

Application of Hairy Root Culture for Bioactive Compounds Production in Medicinal Plants

Li, C., & Wang, M. (2020) · Current Pharmaceutical Biotechnology · doi:10.2174/1389201021666200516155146 · Cited by 36 · see citing works

Indole is an essential molecule for plant interactions with herbivores and pollinators

Cna’ani, A., Seifan, M., & Tzin, V. (2018) · Journal of Plant Biology and Crop Research · doi:10.33582/2637-7721/1003 · Cited by 23 · see citing works

RNAi-mediated modulation of squalene synthase gene expression in Artemisia annua L. and its impact on artemisinin biosynthesis

Ali, A., Ahmad, M. M., Khan, M. A., Saxena, P., Khan, S., & Abdin, M. Z. (2017) · RENDICONTI LINCEI · doi:10.1007/s12210-017-0647-6 · Cited by 17 · see citing works

Systematic engineering of the rate-limiting step of β-alanine biosynthesis in Escherichia coli

Xu, J. Z., Zhu, Y., & Zhou, Z. (2021) · Electronic Journal of Biotechnology · doi:10.1016/j.ejbt.2021.03.002 · Cited by 15 · see citing works

Investigation of the Effects of Artemisinin on Testis and Kidney Injury Induced by Doxorubicin

Tutun, H., Özmen, Ö., Aktaş, İ., Yalçın, A., & Türk, A. (2019) · Acta veterinaria · doi:10.2478/acve-2019-0014 · Cited by 12 · see citing works

In vitro alteration of artemisinin biosynthesis in Artemisia annua L during treatment with methyl jasmonate

Alam, P., & Albalawi, T. (2020) · Tropical Journal of Pharmaceutical Research · doi:10.4314/tjpr.v19i1.5 · Cited by 11 · see citing works

Genome size estimation of Chinese cultured artemisia annua L.

Liu, Z., Guo, S., Xu, J., Zhang, Y., Dong, L., Xiao, S., Bai, R., Liao, B., Su, H., Cheng, R., et al. (2018) · Journal of Plant Biology and Crop Research · doi:10.33582/2637-7721/1002 · Cited by 8 · see citing works

The effect of over-expression of rate limiting enzymes on the yield of artemisinin in Artemisia annua

Alam, P., Kamaluddin, K., Sharafeldin, M. A., Elkholy, S. F., & Abdin, M. Z. (2015) · RENDICONTI LINCEI · doi:10.1007/s12210-015-0481-7 · Cited by 7 · see citing works

Metabolic engineering in Nicotiana benthamiana

Kalalagh, K. F., Papon, N., Courdavault, V., van der Krol, A. R., Kappers, I. F., & Kashkooli, A. B. (2025) · aBIOTECH · doi:10.1007/s42994-025-00234-3 · Cited by 4 · see citing works

Soil microbes and medical metabolites of Artemisia annua L. along altitudinal gradient in Guizhou Karst terrains of China

Luo, S.-Q., Cheng, Z., Zhan-nan, Y., Hu, J., & Di, S.-J. (2019) · Journal of Plant Interactions · doi:10.1080/17429145.2019.1602886 · Cited by 3 · see citing works

Investigating the Use of Biotechnologically Enhanced Medicinal Plants in Allergy Treatment

Mukherjee, R., Dave, M., Panigrahi, J., & Jayaraman, S. (2024) · Springer eBooks · doi:10.1007/978-981-97-1467-4_1 · Cited by 2 · see citing works

In silico identification of cis-Regulatory elements and their functional annotations from assembled ESTs of Artemisia annua L. involved in abiotic stress signaling

Alam, P., & Balawi, T. A. (2021) · ROMANIAN BIOTECHNOLOGICAL LETTERS · doi:10.25083/rbl/26.2/2384.2395 · Cited by 1 · see citing works

Recent Biotechnological Approaches for the Enhancement of Artemisinin Production from Cell Culture System of Artemisia annua L.: Medicinal Plant with Potent Antimalarial Properties

Mahendran, G., & Rahman, L. U. (2022) · Springer protocols handbooks/Springer protocols · doi:10.1007/978-1-0716-2683-2_22 · Cited by 1 · see citing works

Plant Cell Technology

Lee, B. H. (2021) · Wiley eBooks · doi:10.1002/9781119042792.part4

Cellular Engineering for Enhanced Production of Potent Antimalarial Drug: Artemisinin

Ram, M., Niranjan, R., & Jain, D. C. (2013) · Cited by 1 · see citing works

Current state, strategies, and perspectives in enhancing artemisinin production

Zakariya, A. M., Saiman, M. Z., Simonsen, H. T., & Ikram, N. K. K. (2023) · Phytochemistry Reviews · doi:10.1007/s11101-023-09897-6 · Cited by 5 · see citing works

A Review of Biotechnological Artemisinin Production in Plants

Ikram, N. K. K., & Simonsen, H. T. (2017) · Frontiers in Plant Science · doi:10.3389/fpls.2017.01966 · Cited by 130 · see citing works

Artemisinin, a Novel Antimalarial Drug: Biochemical and Molecular Approaches for Enhanced Production

Abdin, M. Z., Israr, M., Rehman, R. U., & Jain, S. K. (2003) · Planta Medica · doi:10.1055/s-2003-38871 · Cited by 252 · see citing works

Searching for Artemisinin Production Improvement in Plants and Microorganisms

Barbacka, K., & Baer‐Dubowska, W. (2011) · Current Pharmaceutical Biotechnology · doi:10.2174/138920111798376923 · Cited by 22 · see citing works